Background: Assessment of the ability to cope with regret can contribute to support strategies for health professionals. However, in Brazil, there are few instruments that evaluate this ability in the general context. The aim of the study was to adapt and validate the Regret Coping Scale for Healthcare Professionals (RCS-HCP) for Brazilian health professionals. Methods: This is a cross-sectional study carried out with professionals linked to tertiary care hospital institutions. In the validation, the instruments were translated, and the psychometric properties were evaluated for validity and reliability. Validity was assessed using the following validities: content (judges' evaluation), criterion (correlation with Satisfaction With Life Scale – SWLS and Self-Reporting Questionnaire-20 – SRQ-20) and construct validity (exploratory factor analysis using the method of Promax rotation, based on the slope graph and Kaiser criterion and confirmatory using the structural equation model). Reliability was measured using Cronbach's α coefficient and the test retested in a maximum period of 30 days. Reproducibility was calculated by intraclass correlation.Results: Three-hundred-forty-one professionals participated, with an average age of 38.6 ± 9.2 years, and 87 participated in a retest survey 30 days later. Exploratory factor analysis showed adequacy of the structure (KMO = 0.786) composed of three factors. In the confirmation, the performance was close to acceptable. Reliability was good for the maladaptive strategies (α = 0.834) and adequate for the problem-focused strategies (α = 0.717), but slightly too low for adaptive strategies (α = 0.595). Test-retest showed lower than expected values, with a Spearman-Brown coefficient of 0.703. Conclusions: The RCS-HCP scale showed satisfactory performance in relation to the properties evaluated.