Stress relaxation amplitude of hydrogels determines migration, proliferation, and morphology of cells in 3-D culture

Hazur, Jonas; Endrizzi, Nadine; Schubert, Dirk W.; Boccaccını, Aldo R.; Fabry, Ben

doi:10.1039/d1bm01089a

Cited by 26 publications

(22 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In skin bioprinting, either primary skin cells such as fibroblasts, melanocytes, keratinocytes, or stem cells are preferred for the fabrication of skin constructs while maintaining all cellular activities after bioprinting [ 209 ]. Another important property of bio-ink includes viscoelastic behavior that influences the skin bioprintability and also affects the cell migration, proliferation, and remodeling of the ECM [ 210 ]. Regardless of the bio-inks or bioprinting techniques that are utilized, skin bioprinting for wound healing applications is carried out using two strategies, namely ex vivo and in situ bioprinting.…”

Section: Innovative Strategies For Wound Healingmentioning

confidence: 99%

Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements

Kolimi

Narala

Nyavanandi

et al. 2022

Cells

184

View full text Add to dashboard Cite

Wound healing is highly specialized dynamic multiple phase process for the repair of damaged/injured tissues through an intricate mechanism. Any failure in the normal wound healing process results in abnormal scar formation, and chronic state which is more susceptible to infections. Chronic wounds affect patients’ quality of life along with increased morbidity and mortality and are huge financial burden to healthcare systems worldwide, and thus requires specialized biomedical intensive treatment for its management. The clinical assessment and management of chronic wounds remains challenging despite the development of various therapeutic regimens owing to its painstakingly long-term treatment requirement and complex wound healing mechanism. Various conventional approaches such as cell therapy, gene therapy, growth factor delivery, wound dressings, and skin grafts etc., are being utilized for promoting wound healing in different types of wounds. However, all these abovementioned therapies are not satisfactory for all wound types, therefore, there is an urgent demand for the development of competitive therapies. Therefore, there is a pertinent requirement to develop newer and innovative treatment modalities for multipart therapeutic regimens for chronic wounds. Recent developments in advanced wound care technology includes nanotherapeutics, stem cells therapy, bioengineered skin grafts, and 3D bioprinting-based strategies for improving therapeutic outcomes with a focus on skin regeneration with minimal side effects. The main objective of this review is to provide an updated overview of progress in therapeutic options in chronic wounds healing and management over the years using next generation innovative approaches. Herein, we have discussed the skin function and anatomy, wounds and wound healing processes, followed by conventional treatment modalities for wound healing and skin regeneration. Furthermore, various emerging and innovative strategies for promoting quality wound healing such as nanotherapeutics, stem cells therapy, 3D bioprinted skin, extracellular matrix-based approaches, platelet-rich plasma-based approaches, and cold plasma treatment therapy have been discussed with their benefits and shortcomings. Finally, challenges of these innovative strategies are reviewed with a note on future prospects.

show abstract

Section: Innovative Strategies For Wound Healingmentioning

confidence: 99%

Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements

Kolimi

Narala

Nyavanandi

et al. 2022

Cells

184

View full text Add to dashboard Cite

show abstract

“…A two-step crosslinking process as described in Figure 1 was designed to gradually introduce Ca 2+ ions to the alginate solution, as compared to inactive calcium sources such as CaCO 3 , CaSO 4 , or CaHPO 4 , which were used in previous studies. [15,[25][26][27][28][29][30][31] In the present approach, fully crosslinked Alg or OA HMPs serve as Ca 2+ reservoirs. Compared to directly pre-crosslinking alginate with a CaCl 2 solution, alginate hydrogels were known to release Ca 2+ ions in a diffusion-controlled release pattern, induced by an ion-exchange process.…”

Section: Preparation Of Hmp Pre-crosslinked Inksmentioning

confidence: 99%

“…[15,[25][26][27][28][29] Other studies have shown that internal gelation can successfully be used for the preparation of bioinks. [30][31][32][33][34] It was also found that pre-crosslinking improves alginate printability by reducing the merging of strands and pores [30,[32][33][34] and that it may also be used to improve cell behavior, such as proliferation and migration [31] or to direct mesenchymal stem cell differentiation. [33] These methods invariably require a change in pH in the gels that cannot be tolerated by every type of cells, e.g., considering previous works of the authors, where D-(+)-glucono-1,5-lacton (GDL) was used as acidifier.…”

Section: Introductionmentioning

confidence: 99%

Pre‐Crosslinking with Hydrogel Microparticles Enhances the Printability of Alginate‐Based Inks

Hazur,

Röder,

Czwalinna

et al. 2023

Macro Materials & Eng

Self Cite

View full text Add to dashboard Cite

The main challenge in extrusion‐based bioprinting is to develop inks which must comprise a manifold of characteristics before, during, and after printing. To tackle the challenge of good shape fidelity and printability of low concentration inks, in this study hydrogel microparticles (HMPs) are proposed to produce internally pre‐crosslinked inks. Alginate (Alg) and oxidized alginate (OA)‐based HMPs are fabricated and used as Ca2+‐releasing reservoirs. OA HMPs are used to demonstrate the versatility of this approach and to show its suitability also for chemically modified alginate. Embedded in either fresh Alg or OA solution, HMPs are used to pre‐crosslink the inks. Rheological measurements revealed that HMP pre‐crosslinking increases the yield stress and viscosity while reducing the loss angle of bioinks. Moreover, printing experiments reveal that being able to tailor rheological properties is an effective tool to improve printability. Furthermore, pre‐crosslinking significantly alters the hydogel internal microstructure. In vitro studies show that NIH/3T3 cells proliferate in HMP pre‐crosslinked bioinks modified with gelatin. Altogether, a low‐cost and easy to use setup to prepare HMPs is presented and for the first time, the possibility of using such HMPs as pre‐crosslinking agent to tailor the printability of alginate‐based bioinks is demonstrated.

show abstract

“…It is important that the printed tissue/organ constantly ensures the normal cellular activities, including cell migration capacity and proliferation rate [13]. Indeed, the viscoelastic behaviour of bioinks influences their printability, but also the ability of cells to remodel the matrix, spread, migrate, and proliferate [14].…”

Section: Introductionmentioning

confidence: 99%

The 3D Bioprinted Scaffolds for Wound Healing

et al. 2022

View full text Add to dashboard Cite

Skin tissue engineering and regeneration aim at repairing defective skin injuries and progress in wound healing. Until now, even though several developments are made in this field, it is still challenging to face the complexity of the tissue with current methods of fabrication. In this review, short, state-of-the-art on developments made in skin tissue engineering using 3D bioprinting as a new tool are described. The current bioprinting methods and a summary of bioink formulations, parameters, and properties are discussed. Finally, a representative number of examples and advances made in the field together with limitations and future needs are provided.

show abstract

Stress relaxation amplitude of hydrogels determines migration, proliferation, and morphology of cells in 3-D culture

Abstract: Fibroblasts spread, migrate and proliferate better in hydrogels with larger stress relaxation amplitude.

Cited by 26 publications

References 27 publications

Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements

Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements

Pre‐Crosslinking with Hydrogel Microparticles Enhances the Printability of Alginate‐Based Inks

The 3D Bioprinted Scaffolds for Wound Healing

Contact Info

Product

Resources

About