Stress resistance for unraveling potential biopollutants. Insights from ballast water community analysis through DNA

Ardura, Alba; Rick, Johannes; Martínez, José Luis; Zaiko, Anastasija; García‐Vázquez, Eva

doi:10.1016/j.marpolbul.2020.111935

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…Therefore, results need to be confirmed using alternative methods, either traditional and/or more sensitive and targeted molecular approaches such as individual barcoding, qPCR or digital PCR (Borrell et al 2018;Harper et al 2018;Wood et al 2019;Ardura et al 2021).…”

Section: Erroneous Species Assignment Challenges the Opportunity To D...mentioning

confidence: 99%

Water eDNA metabarcoding is effective in detecting non-native species in marinas, but detection errors still hinder its use for passive monitoring

et al. 2022

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Marinas are high-priority targets for marine non-indigenous species (NIS), where they compose a large portion of the biofouling communities. The practicality of water samples collection makes environmental DNA (eDNA) metabarcoding an interesting tool for routine NIS surveys. Here the effectiveness of water-eDNA-metabarcoding to identify biofouling NIS, in ten marinas from western France, was examined. Morphological identification of specimens collected in quadrats brought out 18 sessile benthic NIS beneath floating pontoons. Water-eDNA-metabarcoding detected two thirds of them, failing to detect important NIS. However, sampling and bioinformatics filtering steps can be optimized to identify more species. In addition, this method allowed the detection of additional NIS from neighboring micro-habitats. Caution should however be taken when reporting putative novel NIS, because of errors in species assignment. This work highlights that water-eDNAmetabarcoding is effective for active (targeted) NIS surveys, and could be significantly improved for its further use in marine NIS passive surveys.

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Section: Erroneous Species Assignment Challenges the Opportunity To D...mentioning

confidence: 99%

Water eDNA metabarcoding is effective in detecting non-native species in marinas, but detection errors still hinder its use for passive monitoring

et al. 2022

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“…Ardura et al (2021) used stress resistance to identify potentially invasive taxa carried in ballast water during a trans-equatorial expedition. Their results suggest that planktonic taxa that are (i) widely distributed, (ii) capable of reproducing en route, and (iii) able to withstand environmental stressors like oxygen depletion, warming, darkness, and ammonium spikes pose the greatest transplantation or invasion risk ( 108 ). Stress resistance is likely critical in predicting the invasiveness of biofilm taxa, especially since biofilm communities are generally more resistant than their planktonic counterparts ( 6 ).…”

Section: Discussionmentioning

confidence: 99%

Disturbance frequency directs microbial community succession in marine biofilms exposed to shear

Naik,

Kamensky,

Hellum

et al. 2023

mSphere

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Marine biofilm growth poses significant challenges across marine industries (“biofouling”). Understanding the microbial communities involved in biofouling processes is crucial for developing effective mitigation strategies. These communities experience strong disturbances under antifouling pressure, the implications of which must be considered when developing new technologies due to their potential to alter succession, invertebrate settlement, and invasive species establishment risk. We leveraged the development of a shear-based antifouling technique to examine marine biofilm community assembly and stability under disturbance. The influence of repeated underwater shear on microbial community succession and biofilm matrix stability was assessed over 37 days on uncoated and “foul release” paint-coated surfaces. Foul-release coating decreased matrix biomass, and uncoated and coated surfaces hosted different biofilm communities with converging bacterial compositions and diverging eukaryotic compositions over time. On both surfaces, highly frequent shear strongly shifted the community composition and enriched several shear-tolerant bacteria, diatoms, green algae, and ciliates. Infrequent shear decreased matrix biomass, resulted in weaker compositional shifts and fewer enriched taxa, and additionally prevented macrofouling growth when combined with foul-release coating. A cross-domain co-occurrence network revealed mostly positive correlations persisting through the disturbance continuum and identified the diatom Melosira as a highly connected genus. Infrequent shear on anti-biofouling paint-coated surfaces was the most effective biofilm removal strategy, demonstrating that longer recovery periods enabled continued biomass removal and fewer shear-tolerant taxa. The results support the idea that variability in the frequency of a stress disturbance can significantly alter microbial community succession and biomass stability in marine biofilms, resulting in a varied potential for species invasiveness. IMPORTANCE Disturbances are major drivers of community succession in many microbial systems; however, relatively little is known about marine biofilm community succession, especially under antifouling disturbance. Antifouling technologies exert strong local disturbances on marine biofilms, and resulting biomass losses can be accompanied by shifts in biofilm community composition and succession. We address this gap in knowledge by bridging microbial ecology with antifouling technology development. We show that disturbance by shear can strongly alter marine biofilm community succession, acting as a selective filter influenced by frequency of exposure. Examining marine biofilm succession patterns with and without shear revealed stable associations between key prokaryotic and eukaryotic taxa, highlighting the importance of cross-domain assessment in future marine biofilm research. Describing how compounded top-down and bottom-up disturbances shape the succession of marine biofilms is valuable for understanding the assembly and stability of these complex microbial communities and predicting species invasiveness.

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Recent progress and challenges facing ballast water treatment – A review

et al. 2022

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Stress resistance for unraveling potential biopollutants. Insights from ballast water community analysis through DNA

Cited by 7 publications

References 52 publications

Water eDNA metabarcoding is effective in detecting non-native species in marinas, but detection errors still hinder its use for passive monitoring

Water eDNA metabarcoding is effective in detecting non-native species in marinas, but detection errors still hinder its use for passive monitoring

Disturbance frequency directs microbial community succession in marine biofilms exposed to shear

Recent progress and challenges facing ballast water treatment – A review

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