Stress response and tolerance mechanisms of ammonia exposure based on transcriptomics and metabolomics in Litopenaeus vannamei

Xiao, Jie; Li, Qiangyong; Tu, Jia-Peng; Chen, Xiuli; Liu, Qingyun; Liu, Hong; Zhou, Xiaoyun; Zhao, Yang; Wang, Huanling

doi:10.1016/j.ecoenv.2019.05.029

Cited by 83 publications

(45 citation statements)

References 64 publications

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“…Indeed, a previous study suggested that in L. vannamei, high ammonia tolerance was mainly a result of improved ammonia excretion and detoxi cation, as well as an accelerated energy metabolism [17]. Therefore, we speculate that ATP synthesis might affect the ammonia tolerance of L. vannamei by regulating ATP synthesis and controlling cellular ammonia excretion.…”

Section: Discussionmentioning

confidence: 80%

“…At the same time, Lu et al identi ed 202 proteins that were signi cantly differentially expressed between ammonia-tolerant and ammonia-sensitive L. vannamei families using a comparative proteome analysis based on isobaric tags for relative and absolute quanti cation (iTRAQ) [16]. In addition, Jie et al identi ed several pathways and genes involved in ammonia tolerance in L. vannamei based on comparative transcriptomic and metabolomic analyses of ammonia-tolerant and ammonia-sensitive L. vannamei families [17]. Finally, several studies identi ed transcriptomic changes and differentially expressed genes in L. vannamei after ammonia stress [9,18].…”

Section: Introductionmentioning

confidence: 99%

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Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Yang

et al. 2020

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Background: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. Results: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6,360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41–8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. Conclusions: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Yang

et al. 2020

Preprint

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“…Thus, intracellular anion secretion is decreased when SLC26A6 is downregulated, increasing net absorption. In shrimp, previous studies have shown that ammonia secretion is closely related to ammonia tolerance [3]. Ammonia-tolerant shrimp are more able to excrete and detoxify ammonia, while also having an accelerated energy metabolism to provide sufficient energy; these adaptive strategies may increase ammonia stress tolerance [3,48].…”

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

“…The Pacific white shrimp (Litopenaeus vannamei) is the world's most extensively cultivated shrimp species [1,2]; annual production of L. vannamei accounts for about 50% of all penaeid shrimp production [3]. In 2016, the global production of L. vannamei approached 4.16 million tons [3]. However, the intensive or semi-intensive farming model commonly used at present for shrimp aquaculture often leads to water quality deterioration, and the resulting toxicological stress causes mortality or disease in farmed shrimp, negatively affecting breeding efficiency [4].…”

Section: Introductionmentioning

confidence: 99%

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Construction of a high-density genetic map and identification of quantitative trait loci for nitrite tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Chen

Zhu

et al. 2019

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BackgroundNitrite is a major environmental toxin in aquaculture systems disrupting multiple physiological functions in aquatic animals. Although nitrite tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. ResultsIn this study, we constructed a high-density genetic map of L. vannamei with 17,242 single nucleotide polymorphism markers spanning 6,828.06 centimorgans (cM) and an average distance of 0.4 cM between adjacent markers on 44 linkage groups (LGs). Using this genetic map, we identified 2 quantitative trait loci (QTLs) involved in nitrite tolerance on two LGs explaining 8.42–10.31% of the phenotypic variance. We then sequenced the transcriptomes of the most nitrite-tolerant and the most nitrite-sensitive individuals from each of four genetically distinct L. vannamei families and found 2,002, 1,983, 1,954, and 1,867 differentially expressed genes in the four families, respectively. By integrating QTL and transcriptomics analyses, we identified a candidate gene associated with nitrite tolerance. This gene was annotated as solute carrier family 26 member 6 (SLC26A6). RNA interference (RNAi) analysis demonstrated that SLC26A6 was critical for nitrite tolerance in L. vannamei. ConclusionsThe present study increases our understanding of the molecular mechanisms underlying nitrite tolerance in shrimp, and provides a basis for molecular-marker-assisted shrimp breeding.

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Advanced Oxidation Processes‐Mediated Removal of Aqueous Ammonia Nitrogen in Wastewater

Aslam¹,

Abdullah²,

Ahmed³

et al. 2023

Photoreactors in Advanced Oxidation Processes

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Stress response and tolerance mechanisms of ammonia exposure based on transcriptomics and metabolomics in Litopenaeus vannamei

Cited by 83 publications

References 64 publications

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Identification of a quantitative trait loci (QTL) associated with ammonia tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Construction of a high-density genetic map and identification of quantitative trait loci for nitrite tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Advanced Oxidation Processes‐Mediated Removal of Aqueous Ammonia Nitrogen in Wastewater

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