Stress Responses of Neonates Related to Maternal Characteristics

Joung, Kyoung Hwa; Cho, Soo-Chul

doi:10.3349/ymj.2011.52.1.98

Cited by 6 publications

(4 citation statements)

References 24 publications

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“…To investigate the specific mechanism of cell proliferation of negative pressure-treated MSCs, ER stress-associated proteins (UPR pathway) (25,26), including p-Perk, IRE1 and cleaved ATF6 levels, were detected. Three proteins were demonstrated to be activated when treated with negative pressure.…”

Section: Discussionmentioning

confidence: 99%

Functions and mechanisms of intermittent negative pressure for osteogenesis in human bone marrow mesenchymal stem cells

Yang

Zhang

et al. 2014

Molecular Medicine Reports

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The present study aimed to determine the mechanism by which low‑intensity intermittent negative pressure affects the differentiation and proliferation of human mesenchymal stem cells (MSCs). Alkaline phosphatase (ALP) activity, type I collagen and vascular endothelial growth factor (VEGF) were detected to analyze differentiation. MTT and flow cytometry were employed to measure proliferation and apoptosis. Western blot analysis was used to examine endoplasmic reticulum (ER) stress‑associated factors. This study was divided into two groups, including a normal group (without any treatment) and vacuum group (treated with a vacuum). There was a significant decrease in the proliferation of cells in the vacuum group. The number of cells in S phase was reduced significantly, while the rate of apoptosis and the activity of ALP were markedly increased under vacuum conditions. Expression of collagen type I and VEGF was significantly increased, and the ratio of osteoprotegrin to osteoprotegrin ligand was decreased significantly in the vacuum group. ER stress‑associated proteins, p‑PRKR‑like ER kinase, inositol‑requiring enzyme 1 and cleaved activating transcription factor 6, as well as the downstream factors, were activated when treated with negative pressure. In conclusion, treatment with low‑intensity and intermittent negative pressure may inhibit the proliferation of MSCs and trigger ER stress‑associated cellular apoptosis, further enhancing osteogenesis activity and inducing differentiation to osteoblasts.

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Section: Discussionmentioning

confidence: 99%

Functions and mechanisms of intermittent negative pressure for osteogenesis in human bone marrow mesenchymal stem cells

Yang

Zhang

et al. 2014

Molecular Medicine Reports

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“…There is an increasing evidence that ER stress acts a significant function in the apoptosis regulation. Two specific signaling pathways were involving the ER stress process, such as, unfolded protein responses (UPR) and ER-associated protein degradation7,8 UPR pathway participates in the activation of some specific proteins, including activating transcription factor 6 (ATF6), PKR-like ER kinase (PERK), and inositol requiring proteins 1 (IRE1) 9. The above three pathways of UPR activate several transcription factors, such as eukaryotic translation initiation factor-2α (eIF-2α) and X-box transcription factor-1 (XBP1).…”

Section: Introductionmentioning

confidence: 99%

Human Leptin Protein Induces Proliferation of A549 Cells via Inhibition of PKR-Like ER Kinase and Activating Transcription Factor-6 Mediated Apoptosis

Lai

Sun

2013

Yonsei Med J

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PurposeTo investigate the anti-apoptotic mechanism of leptin in non-small cell lung cancer.Materials and MethodsThe influences of leptin on apoptosis were investigated, analyzing the mechanism that triggers growth of A549 cells. The effects of leptin on cell proliferation were examined by XTT analysis. Leptin, C/EBP homologous protein (CHOP), phosphorylated-PKR-like ER kinase (p-Perk), inositol requiring proteins-1, spliced X-box transcription factor-1 (XBP1), cleaved activating transcription factor-6 (ATF6), eukaryotic translation initiation factor-2α, caspase-12 and CHOP protein were detected in four groups by western blot, and endoplasmic reticulum (ER) stress related mRNA were detected by reverse transcription PCR.ResultsThe expression of leptin in A549 and leptin transfected cells inhibited cisplatin activated ER stress-associated mRNA transcription and protein activation. Two ER stress unfolded protein response pathways, PERK and ATF6, were involved, and XBP1 and tumor necrosis factor receptor-associated factor 2 (TRAF2) were increased significantly when treated with cisplatin in A549-siRNA against leptin cells. Furthermore, CHOP expression was inhibited upon leptin expression in A549, LPT-PeP and LPT-EX cells.ConclusionLeptin serves as an important factor that promotes the growth of A549 cells through blocking ER stress-mediated pathways. This blocking is triggered by p-Perk and ATF6 via inhibition of CHOP expression.

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“…Amylase is also used as a psychological stress biomarker 22 along with chromogranin A 23 and cortisol. 24,25 The increase in amylase was not linked to the chromogranin A (Fig. 2) or cortisol concentrations (data not shown).…”

Section: Discussionmentioning

confidence: 84%

Clinical Evaluation of Dental Metal Hazards and Therapeutic Effects of Shortwave Diathermy in Dental Medicine

Mukai¹,

Nakamoto²,

Kondo³

et al. 2012

International Journal of Prosthodontics and Restorative Dentistry

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Objective Shortwave diathermy causes increase in temperature from deep inside the body, results in upregulation of metabolism, and has analgesic effects. In this study, we explored the potential application of shortwave diathermy in the maxillofacial region by monitoring internal and external temperature changes, changes in blood flow, and resting saliva secretion under shortwave diathermy for subjects with and without dental metal restorations (MR) to confirm the safety and the limitation of shortwave diathermy for dental medicine. Materials and methods Twenty young healthy subjects were recruited (10 subjects with MR and 10 subjects without MR). Shortwave exposure was achieved with condenser-type probes placed on the bilateral mandibular angles, and the intraoral temperature was monitored. For functional analysis, unstimulated whole saliva before and after exposure was collected. The temperature and blood flow distributions of the exposed areas were then monitored by thermography and twodimensional (2D) laser Doppler flowmetry. Results Shortwave exposure for 20 minutes induced significant temperature increases in all groups (p ≤ 0.05). The subjects reported no discomfort. When subjects without MR held gold or titanium crowns in their mouths, the thermal effect by shortwave diathermy was reduced. Resting saliva upon exposure only increased significantly in metal-free subjects, and amylase concentration was also increased, but the level of the salivary stress biomarker chromogranin A was unchanged. Furthermore, thermography showed that shortwave diathermy significantly increased the surface temperature for cheek skin and the buccal mucosa; however, this increase failed to elevate surface blood flow. Conclusion Shortwave diathermy induces temperature increases in the maxillofacial region. MR did not cause excessive heating, on the contrary, reduce the thermal effect of shortwave radiation. The temperature rise maintains upregulation in salivary function, which could be utilized safely in dental medicine. How to cite this article Mukai C, Nakamoto T, Kondo Y, Masaki C, Ohta A, Hosokawa R. Clinical Evaluation of Dental Metal Hazards and Therapeutic Effects of Shortwave Diathermy in Dental Medicine. Int J Prosthodont Restor Dent 2012;2(3): 88-95.

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Stress Responses of Neonates Related to Maternal Characteristics

Cited by 6 publications

References 24 publications

Functions and mechanisms of intermittent negative pressure for osteogenesis in human bone marrow mesenchymal stem cells

Functions and mechanisms of intermittent negative pressure for osteogenesis in human bone marrow mesenchymal stem cells

Human Leptin Protein Induces Proliferation of A549 Cells via Inhibition of PKR-Like ER Kinase and Activating Transcription Factor-6 Mediated Apoptosis

Clinical Evaluation of Dental Metal Hazards and Therapeutic Effects of Shortwave Diathermy in Dental Medicine

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