Stress responsiveness of vindoline accumulation in Catharanthus roseus leaves is mediated through co-expression of allene oxide cyclase with pathway genes

Temperature‐induced lipocalin‐mediated membrane integrity: Possible implications for vindoline accumulation in Catharanthus roseus leaves

“…A comparison of the expression of CrTIL (from this study) and CrAOC , as well as vindoline metabolism‐related genes and vindoline content under various biotic/abiotic factors (Mall, Shanker, et al, 2022) was carried out. CrTIL expression under various biotic factors (bacterial endophyte, phytoplasma, CMV) and some abiotic factors (cold, salt, and SA) followed the same pattern as that of CrAOC and most of the vindoline biosynthesis‐related genes even though the decrease in CrTIL expression in the case of SA treatment uniquely showed an oscillatory pattern as time elapsed.…”

“…C. roseus plants were treated with three biotic [bacterial endophytes, phytoplasma, cucumber mosaic virus (CMV)] and five abiotic [high/low temperature, salt, salicylic acid (SA), methyl jasmonate (MeJa)] factors exactly as described earlier (Mall, Shanker, et al, 2022). Experiments were carried out on three biological replicates, whereby each biological replicate was technically replicated thrice.…”

“…Inoculated plants were maintained in a glass house and sampled at maturity (6‐month‐old). The group II phytoplasma isolated earlier from C. roseus (Mall, Shanker, et al, 2022) was used for phytoplasma treatment. Two‐month‐old healthy plants were graft‐inoculated with the phytoplasma using scions of an infected plant following the method of Kamińska and Sliwa (2005) and maintained in an insect‐proof greenhouse.…”

“…The symptoms of phytoplasma infection became evident ~2–3 months post‐grafting and plants were sampled at maturity (6‐month‐old). For CMV treatment, the virus isolate (strain CR1; Mall, Shanker, et al, 2022) was sap‐transmitted to 2‐month‐old healthy C. roseus plants, whereby symptoms of infection emerged ~28 days post‐inoculation. The plants were maintained in an insect‐proof greenhouse and sampled at maturity (6‐month‐old).…”

“…Recently, C. roseus allene oxide cyclase ( CrAOC ), a lipocalin family member, has been shown to co‐express with vindoline pathway genes in C. roseus under various biotic/abiotic stresses (Mall, Shanker, et al, 2022). This brought into focus another family member in C. roseus , a temperature‐induced lipocalin ( CrTIL ), which has been characterized for its function and localization as well as its effect on vindoline (that needs to cross an intracellular membrane for sequestration and storage) accumulation in plant leaves in the present study.…”