Industrial buildings usually are designed to sustain several types of load systems, such as dead, live, and dynamic loads (especially the harmonic load produced by rotary motors). In general, these buildings require high-strength structural elements to carry the applied loads. Moreover, Reactive Powder Concrete (RPC) has been used for this purpose because of its excellent mechanical strength and endurance. Therefore, this study provides an experimental analysis of the structural behaviors of reinforced RPC beams under harmonic loads. The experimental program consisted of testing six simply supported RPC beams with lengths of 1500 mm, widths of 150 mm, and thicknesses of 200 mm under harmonic loading with varied frequencies between 10 and 20 Hz. Different steel fiber ratios of 0%, 0.5%, 0.75%, 1.0%, 1.5%, and 1.75% were provided in the concrete mixes to explore the effect of steel fibers on the dynamic behavior of these beams. Except for the steel fiber volume fraction, all of the examined specimens shared the same material attributes and reinforcing details. The outcomes proved the positive effect of adding steel fibers on the dynamic response under the effect of harmonic loading. The optimum volume fraction of steel fibers was characterized by a percentage of 1.5%. Moreover, the vibration amplitude was more affected by the steel fibers than the support reactions. The inertial force increased as the harmonic loading duration increased. This increase in the inertial force by the load duration was enhanced after adding the steel fibers. However, this enhancement started to decline after increasing the steel fiber content to 1.75%.