Stretch-Induced Healing of Injured Muscles Is Associated With Myogenesis and Decreased Fibrosis

Hu, Chih-Chien; Chen, Carl Pai-Chu; Tsui, Po-Hsiang; Chen, Chiao‐Nan; Hsu, Chih‐Chin

doi:10.1177/03635465221083995

Cited by 1 publication

(1 citation statement)

References 37 publications

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“…The fibrotic scar produced is important for tissue integrity during the early phases of muscle healing; however, its presence in later stages has a detrimental effect on muscle function [ 38 ]. Therefore, the development of therapies aimed at reducing muscle fibrosis may be promising, such as stretching exercises [ 42 , 43 , 44 ] or the use of drugs targeting pro-fibrotic cytokines [ 45 , 46 , 47 ] that have been shown to improve muscle healing by reducing muscle fibrosis and increasing myogenesis. Several studies have shown that physical exercise and stretching have an effect on mesenchymal stem cells (MSCs) through mechanotransduction, converting the mechanical loading into different cellular responses that have an impact on improving muscle regeneration [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel Minimally Invasive Surgically Induced Skeletal Muscle Injury Model in Sheep

Vidal,

Vila,

Venegas

et al. 2024

IJMS

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Sports-related muscle injuries account for 10–55% of all injuries, which is a growing concern, especially given the aging world population. To evaluate the process of skeletal muscle injury and compare it with muscle lesions observed in humans, we developed a novel in vivo model in sheep. In this model, muscle injury was induced by an ultrasound-guided transverse biopsy at the myotendinous junction of the medial gastrocnemius muscle. Twelve male sheep were examined at 3, 7, 14, and 28 days post-injury. Histological, immunofluorescence, and MRI analyses indicate that our sheep model could resemble key human clinicopathological features. Statistically significant differences (p < 0.05) were observed in collagen I, dMHC, α-SMA, and CD68 immunohistochemical detection when comparing injured and healthy muscles. The injured gastrocnemius muscle exhibited elevated levels of type I collagen, infiltration of CD68(+) macrophages, angiogenesis, and the emergence of newly regenerated dMHC(+) myofibers, which persisted for up to 4 weeks post-injury. Similarly, the progression of muscle injury in the sheep model was assessed using advanced clinical 3 T MRI and compared with MRI scans from human patients. The data indicate that the sheep muscle injury model presents features similar to those observed in human skeletal muscle injuries. This makes it a valuable large animal model for studying muscle injuries and developing novel therapeutic strategies.

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