Strigol induces germination of the facultative parasitic plant <i>Phtheirospermum japonicum</i> in the absence of nitrate ions

Ogawa, Satoshi; Shirasu, Ken

doi:10.1080/15592324.2022.2114647

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…Plant roots and AMF respond to each other in the course of symbiosis (Dhanker et al, 2020;Nasir et al, 2021), and the roots and AMF are capable of mutual tropism growth. We analyzed the host tropism of AMF (Sbrana and Giovannetti, 2005;Pineda-Martos et al, 2021;Ogawa and Shirasu, 2022a;Ogawa et al, 2022b), and our preliminary results suggest that the host tropism of spores was significantly inhibited by myristate application. The main purpose of this study was to explore the mycorrhizal colonization of AMF, so the hairy roots and spores were not cultured in separate compartments, and we only observed and analyzed the phenomenon of the host tropism in AMF preliminarily.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we observed that the hairy roots and AMF have the ability to grow toward each other, and called the ability of AMF to grow toward host plant roots as host tropism (Ogawa and Shirasu, 2022a;Sbrana and Giovannetti, 2005;Pineda-Martos et al, 2021;Ogawa et al, 2022b). In addition, some roots grew toward AMF, and the germ tube could contact the roots after spore germination immediately.…”

Section: Sand Culture System (Experiments 2)mentioning

confidence: 96%

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Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

Liu,

Feng,

Zhang

et al. 2023

Front. Plant Sci.

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Arbuscular mycorrhizal fungi (AMF) can establish symbiotic associations with the roots of most terrestrial plants, thereby improving the tolerance of the host plants to biotic and abiotic stresses. Although AMF cannot synthesize lipids de novo, they can obtain lipids from the root cells for their growth and development. A recent study reveals that AMF can directly take up myristate (C14:0 lipid) from the environment and produce a large amount of hyphae in asymbiotic status; however, the effect of environmental lipids on AM symbiosis is still unclear. In this study, we inoculated tomato (Solanum lycopersicum) with AMF in an in vitro dual culture system and a sand culture system, and then applied exogenous myristate to the substrate, in order to explore the effect of exogenous lipids on the mycorrhizal colonization of AMF. We investigated the hyphae growth, development, and colonization of AMF, and examined the gene expression involved in phosphate transport, lipid biosynthesis, and transport. Results indicate that exogenous lipids significantly stimulated the growth and branching of hyphae, and significantly increased the number of hyphopodia and mycorrhizal colonization of AMF, with arbuscular abundance and intraradical spores or vesicles being the most promoted. In contrast, exogenous myristate decreased the growth range and host tropism of the germ tubes, and largely inhibited the exchange of nutrition between symbionts. As a result, exogenous myristate did not affect the plant growth. This study suggests that lipids promote mycorrhizal colonization by enhancing the growth and development of AMF hyphae and increasing their contact opportunities with plant roots. To the best of our knowledge, this is the first report that shows that lipids promote the colonization of AMF. Our study highlights the importance of better understanding the roles of environmental lipids in the establishment and maintenance of AM symbiosis and, thus, in agricultural production.

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Section: Discussionmentioning

confidence: 99%

Section: Sand Culture System (Experiments 2)mentioning

confidence: 96%

Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

Liu,

Feng,

Zhang

et al. 2023

Front. Plant Sci.

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Strigolactones initiate the formation of haustorium-like structures in Castilleja

Bürger,

Peterson,

Chory

2024

iScience

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A Divergent Clade KAI2 Protein in the Root Parasitic Plant Orobanche minor Is a Highly Sensitive Strigolactone Receptor and Is Involved in the Perception of Sesquiterpene Lactones

Takei

Uchiyama

Bürger

et al. 2023

Plant and Cell Physiology

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Strigolactones (SLs) were initially discovered as germination inducers for root parasitic plants. In 2015, three groups independently reported the characterization of the SL receptor in the root parasitic plant Striga hermonthica, which causes significant damage to crop production, particularly in sub-Saharan Africa. The characterized receptors belong to HYPOSENSITIVE TO LIGHT/KARRIKIN INSENSITIVE2 (HTL/KAI2), which is a member of the α/β-hydrolase protein superfamily. In non-parasitic plants, HTL/KAI2 perceives the smoke-derived germination inducer karrikin and a yet-unidentified endogenous ligand. However, root parasitic plants evolved a specific clade of HTL/KAI2 that has diverged from the KAI2 clade of non-parasitic plants. The S. hermonthica SL receptors are included in this specific clade, which is called KAI2 divergent (KAI2d). Orobanche minor is an obligate root holoparasitic plant that grows completely dependent on the host for water and nutrients because of a lack of photosynthetic ability. Previous phylogenetic analysis of KAI2 proteins in O. minor has demonstrated the presence of at least five KAI2d clade genes. Here, we report that KAI2d3 and KAI2d4 in O. minor have the ability to act as the SL receptors. They directly interact with SLs in vitro, and when expressed in Arabidopsis, they rescue thermo-inhibited germination in response to the synthetic SL analog GR24. In particular, KAI2d3 showed high sensitivity to GR24 when expressed in Arabidopsis, suggesting that this receptor enables highly sensitive SL recognition in O. minor. Furthermore, we provide evidence that these KAI2d receptors are involved in the perception of sesquiterpene lactones, non-strigolactone-type germination inducers.

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Strigol induces germination of the facultative parasitic plant Phtheirospermum japonicum in the absence of nitrate ions

Cited by 7 publications

References 33 publications

Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

Exogenous myristate promotes the colonization of arbuscular mycorrhizal fungi in tomato

Strigolactones initiate the formation of haustorium-like structures in Castilleja

A Divergent Clade KAI2 Protein in the Root Parasitic Plant Orobanche minor Is a Highly Sensitive Strigolactone Receptor and Is Involved in the Perception of Sesquiterpene Lactones

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