Strigolactone Biosynthesis in <i>Medicago</i>  <i>truncatula</i> and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2

Liu, Wei; Kohlen, Wouter; Lillo, Alessandra; Camp, Rik Op den; Ivanov, Sergey; Hartog, Marijke; Limpens, Erik; Jamil, Muhammad; Smaczniak, Cezary; Kaufmann, Kerstin; Yang, Wei‐Cai; Hooiveld, Guido; Charnikhova, Tatsiana; Bouwmeester, Harro J.; Bisseling, Ton; Geurts, René

doi:10.1105/tpc.111.089771

Cited by 292 publications

(255 citation statements)

References 57 publications

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“…Pea (Pisum sativum) mutants deficient in BRs or GA had reduced nodulation (Ferguson et al, 2005), and SL levels were positively correlated with nodulation in pea and L. japonicus (Soto et al, 2010;Foo and Davies, 2011;Foo and Reid, 2013;Liu et al, 2013), but infection was not characterized in these studies. Our observation that CCD8 is induced in root hairs by S. meliloti taken together with the identification of SLs in M. truncatula root exudates (Liu et al, 2011) raises the possibility that SLs are secreted during infection. Therefore, SLs may either act as an additional cue for rhizobia in the rhizosphere, as seen with mycorrhization (Akiyama et al, 2005), or they may induce developmental changes in root hairs during infection, perhaps analogous to their role in induction of lateral root formation.…”

Section: Discussionmentioning

confidence: 56%

The Root Hair “Infectome” of Medicago truncatula Uncovers Changes in Cell Cycle Genes and Reveals a Requirement for Auxin Signaling in Rhizobial Infection

et al. 2014

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Section: Discussionmentioning

confidence: 56%

The Root Hair “Infectome” of Medicago truncatula Uncovers Changes in Cell Cycle Genes and Reveals a Requirement for Auxin Signaling in Rhizobial Infection

et al. 2014

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“…GRAS-domain proteins have already been shown to function during AM associations, with RAM1 playing a major role [6], and NSP1/NSP2 playing minor roles [7][8]. We found that OsRAM1 can complement Medicago ram1, indicating that OsRAM1 served the same function as MtRAM1 during mycorrhizal colonization (Supplementary information, Figure S7).…”

Section: Dear Editormentioning

confidence: 51%

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

et al. 2013

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“…Isolation, identification and quantification of petunia strigolactones. Plants were grown in an X-Stream 20 aeroponic system (Nutriculture) as previously described for Medicago truncatula 47 . From day 8 until day 12, exudates were collected and pooled, and root material was sampled.…”

Section: Methodsmentioning

confidence: 99%

A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching

Kretzschmar

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et al. 2012

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Strigolactones were originally identified as stimulators of the germination of root-parasitic weeds 1 that pose a serious threat to resource-limited agriculture 2 . They are mostly exuded from roots and function as signalling compounds in the initiation of arbuscular mycorrhizae 3 , which are plant-fungus symbionts with a global effect on carbon and phosphate cycling 4 . Recently, strigolactones were established to be phytohormones that regulate plant shoot architecture by inhibiting the outgrowth of axillary buds 5,6 . Despite their importance, it is not known how strigolactones are transported. ATP-binding cassette (ABC) transporters, however, are known to have functions in phytohormone translocation [7][8][9] . Here we show that the Petunia hybrida ABC transporter PDR1 has a key role in regulating the development of arbuscular mycorrhizae and axillary branches, by functioning as a cellular strigolactone exporter. P. hybrida pdr1 mutants are defective in strigolactone exudation from their roots, resulting in reduced symbiotic interactions. Above ground, pdr1 mutants have an enhanced branching phenotype, which is indicative of impaired strigolactone allocation. Overexpression of Petunia axillaris PDR1 in Arabidopsis thaliana results in increased tolerance to high concentrations of a synthetic strigolactone, consistent with increased export of strigolactones from the roots. PDR1 is the first known component in strigolactone transport, providing new opportunities for investigating and manipulating strigolactone-dependent processes.Strigolactones are a new class of carotenoid-derived 10 phytohormone in land plants. In addition to their role in shoot branching, strigolactones are exuded into the rhizosphere under phosphorus-limiting conditions 5 and act as growth stimulants of arbuscular mycorrhizal fungi 3 . To identify efflux carriers of arbuscular-mycorrhiza-promoting factors such as strigolactones, we used a degenerate primer approach ( Supplementary Fig. 2a) to isolate full-size PDR-type transporters (also known as ABC subtype G (ABCG) transporters) of P. hybrida that are abundant in phosphate-starved or mycorrhizal roots. The rationale behind the focus on these transporters, of which there are 15 in Arabidopsis 11 , 23 in Oryza sativa (rice) 11 and 23 putative factors in Solanum lycopersicum (tomato) ( Supplementary Fig. 3a), was that they are plasma membrane proteins often found in roots 12 , they are implicated in below-ground plantmicrobe interactions 13,14 , and they have affinities for compounds that are structurally related to strigolactones 8,9,15 . Of six primary candidates, only P. hybrida PDR1 had increased expression in roots that were subjected to either phosphate starvation (Fig. 1a) or colonization by the arbuscular mycorrhizal fungus Glomus intraradices (Fig. 1b). Furthermore, PDR1 transcript levels increased in response to treatment with the synthetic strigolactone analogue GR24 or the auxin analogue 1-naphthaleneacetic acid (NAA) (Fig. 1c). Auxin has been shown to upregulate strigolactone-bi...

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Strigolactone Biosynthesis in Medicago truncatula and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2

Cited by 292 publications

References 57 publications

The Root Hair “Infectome” of Medicago truncatula Uncovers Changes in Cell Cycle Genes and Reveals a Requirement for Auxin Signaling in Rhizobial Infection

The Root Hair “Infectome” of Medicago truncatula Uncovers Changes in Cell Cycle Genes and Reveals a Requirement for Auxin Signaling in Rhizobial Infection

A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants

A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching

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