Stripe rust resistance and dough quality of new wheat - Dasypyrum villosum translocation lines

Abstract

ABSTRACT. The transfer of agronomically useful genes from wild wheat species into cultivated wheat is one of the most effective approaches to improvement of wheat varieties. To evaluate the transfer of genes from Dasypyrum villosum into Triticum aestivum, wheat quality and disease resistance was evaluated in two new translocation lines, T1DL•1V#3S and T1DS•1V#3L. We examined the levels of stripe rust resistance and dough quality in the two lines, and identified and located the stripe rust resistant genes and high molecular weight glutenin subunit (HMW-GS) genes Glu-V1 of D. villosum. Compared to the Chinese Spring (CS) variety, T1DL•1V#3S plants showed moderate resistance to moderate susceptibility to the stripe rust races CYR33 and Su11-4. However, T1DS•1V#3L plants showed high resistance or 8077-8083 (2015) immunity to these stripe rusts. The genes for resistance to stripe rust were located on 1VL of D. villosum. In comparison to CS, the dough from T1DS•1V#3L had a significantly shorter developing time (1.45 min) and stable time (1.0 min), a higher weakness in gluten strength (208.5 FU), and a lower farinograph quality index (18). T1DL•1V#3S had a significantly longer developing time (4.2 min) and stable time (5.25 min), a lower weakness in gluten strength (53 FU) and a higher farinograph quality index (78.5). We also found that T1DS•1V#3L had reduced gluten strength and dough quality compared to CS, but T1DL•1V#3S had increased gluten strength and dough quality. The results of SDS-PAGE analysis indicated that Glu-V1 of D. villosum was located on short arm 1VS and long arm 1VL. These results prove that the new translocation lines, T1DS•1V#3L and T1DS•1V#3L, have valuable stripe rust resistance and dough quality traits that will be important for improving wheat quality and resistance in future wheat breeding programs.

Molecular Cytogenetic Characterization of New Wheat—Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat

“…Genes at locus Glu-V1 for HMW storage protein subunits are located on chromosome 1V, and prolamin genes are located on chromosomes 1V and 4V ( Montebove et al, 1987 ; De Pace et al, 2001 ; Vaccino et al, 2010 ). The recently identified two wheat— D. villosum homozygous translocations confirmed that the HMW-GS gene of D. villosum was located on both 1VL and 1VS ( Dong et al, 2013 ; Zhang et al, 2014 ; Zhao et al, 2015 ). In the present study, we also cloned ten sequences from Dasypyrum sequences and identified the different lengths of HMW-GS sequences in both D. breviaristatum and the wheat— D. breviaristatum partial amphiploid TDH-2.…”

“…Dasypyrum species possess agronomically important genes controlling traits including disease resistance, high protein contents and drought adaptation, which represent valuable resources for wheat improvement over the world ( Zhang et al, 2005 ; De Pace et al, 2011 ). The annual species Dasypyrum villosum carries effective resistance genes to several fungal pathogens of wheat and has genes which may have a positive contribution to quality improvement when transferred into a wheat background ( Chen et al, 1995 ; Patrizia et al, 2010 ; Qi et al, 2011 ; Zhao et al, 2010 , 2015 ). It is thus essential to localize the novel seed proteins from D. villosum and to characterize the HMW-GS genes from wheat— D. villosum derivatives ( Zhong and Qualset, 1993 ; Vaccino et al, 2010 ; Zhang et al, 2014 ; Yang et al, 2014 ).…”

Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

“…In 2000, Yildirim et al [ 17 ] tested the stripe rust reactions of D. villosum accessions, and the results showed that, of the 115 D. villosum accessions tested, 41 (35.65%) were resistant to stripe rust, indicating that D. villosum provides a vast pool of genes for stripe rust resistance. However, there only a few stripe rust resistance genes have been explored in D. villosum [ 52 ].…”