Stroke promotes survival of nearby transplanted neural stem cells by decreasing their activation of caspase 3 while not affecting their differentiation

Kosi, Nina; Alić, Ivan; Salamon, Iva; Mitrečić, Dinko

doi:10.1016/j.neulet.2017.12.040

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…Moreover, TLR2 activation leads to the activation of the NF-κB pathway (Rolls et al, 2007), which may be another cause of increased iNOS in the diabetic group. GAP-43 is associated with presynaptic neuronal outgrowth and neuronal plasticity and regulates the organization of presynaptic terminal and neurotransmitter release (Kosi et al, 2018). In contrast to our findings, Zhou et al (2007) have shown a decreased hippocampal expression of GAP43 in diabetic rats.…”

Section: For the Hippocampal Parameterscontrasting

confidence: 99%

Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic Murine Model

et al. 2021

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Diabetes mellitus (DM) is a multisystem endocrine disorder affecting the brain. Mesenchymal stem cells (MSCs) pretreated with Melatonin have been shown to increase the potency of MSCs. This work aimed to compare Melatonin, stem cells, and stem cells pretreated with Melatonin on the cognitive functions and markers of synaptic plasticity in an animal model of type I diabetes mellitus (TIDM). Thirty-six rats represented the animal model; six rats for isolation of MSCs and 30 rats were divided into five groups: control, TIDM, TIDM + Melatonin, TIDM + Stem cells, and TIDM + Stem ex vivo Melatonin. Functional assessment was performed with Y-maze, forced swimming test and novel object recognition. Histological and biochemical evaluation of hippocampal Neuroligin 1, Sortilin, Brain-Derived Neurotrophic Factor (BDNF), inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2), Tumor necrosis factor-alpha (TNF-α), and Growth Associated Protein 43 (GAP43). The TIDM group showed a significant decrease of hippocampal Neuroligin, Sortilin, and BDNF and a significant increase in iNOS, TNF-α, TLR2, and GAP43. Melatonin or stem cells groups showed improvement compared to the diabetic group but not compared to the control group. TIDM + Stem ex vivo Melatonin group showed a significant improvement, and some values were restored to normal. Ex vivo melatonin-treated stem cells had improved spatial working and object recognition memory and depression, with positive effects on glucose homeostasis, inflammatory markers levels and synaptic plasticity markers expression.

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Section: For the Hippocampal Parameterscontrasting

confidence: 99%

Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic Murine Model

et al. 2021

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“…We have already shown that different stem cell populations exhibit different beneficial effects on disease-affected nervous tissue ( 25 ). Although some studies, both on preclinical and clinical levels, described mechanisms responsible for beneficial effects, including effects on transplanted cells ( 26 ), many of them focused on description of cell fates ( 27 ) or reported beneficial effects, while the mechanisms themselves remained elusive. Most studies analyzing cell death reported only one or a few markers, mostly focusing on classic apoptotic pathways, like CASP3 ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Transplantation of neural stem cells in the mouse model of ischemic brain stroke and expression of genes involved in programmed cell death

et al. 2018

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AimTo analyze how neural stem cells (NSC) transplantation in the stroke-affected mouse brain influences the expression of genes involved in apoptosis-inducing factor (AIF)-mediated cell death – apoptosis inducing factor mitochondria associated 1 (Aifm1), ring finger protein 146 (Rnf146, Iduna), and cyclophilin A (CypA); necroptosis –receptor interaction protein kinase 1 (Ripk1), Ripk3, and mixed-lineage kinase domain-like protein (Mlkl); and apoptosis – Caspase 3 (Casp3) and Casp8.MethodsFour groups of animals were used to obtain mRNA for quantitative reverse transcription polymerase chain reaction analysis: healthy animals (n = 3), animals with stroke (n = 4), animals with stroke treated by stem cell transplantation (n = 7), and animals with stroke treated by proliferation-supporting medium (n = 5). Ischemic brain injury was induced by transient left middle cerebral artery occlusion. Statistical analysis was performed using one-way analysis of variance with post-hoc Tukey test.ResultsNSC transplantation in the stroke-affected mouse brain significantly increased the expression of Iduna (P < 0.05), a gene-encoding protein with well-known protective effects on hypoxic damage, and significantly down-regulated the expression of damage-supportive genes, Casp3 (P < .01) and Aifm1 (P < 0.001). We were able to distinguish between the effect produced by stem cell transplantation (Iduna, Aifm1, Ripk3, Mlkl) and the effect produced by supporting the tissue with proliferation-supporting medium (Ripk1, Casp8).ConclusionBeside revealing some clearly positive effects of stem cells transplantation on the stroke-affected brain, our results suggest that the tissue response triggered by stem cells points toward the desired, regeneration-supporting levels of expression of a certain gene at a certain time point.

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“…An in-vivo magnetic resonance imaging (MRI) study has been performed using C57Bl6 mouse in whom stroke has been induced by MCAO method, as reported by our group 24 . Upon isolation of T-lymphocytes from blood plasma obtained through orbital venous sinus bleeding, the cells were separated in two groups.…”

Section: Methodsmentioning

confidence: 99%

Modeling of inhomogeneous electromagnetic fields in the nervous system: a novel paradigm in understanding cell interactions, disease etiology and therapy

Isakovic

Dobbs-Dixon

Chaudhury

et al. 2018

Sci Rep

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All major processes in the nervous system depend on interactions between cells and nerve fibers. In this work we present a novel model of inhomogeneous electromagnetic fields originating from nerve fibers and delineate their influence on cells. By expanding Hodgkin-Huxley’s applied current into axial current, governed by, we reveal that cell-with-neuron interactions are regulated by the strength of the electromagnetic fields, which are homogeneous up to 2.066 μm or 6.606 μm away from neurilemma and axolemma, respectively. At the nodes of Ranvier, these fields reach strengths of 3.0 × 10−12T, while at the myelinated segments they only peak at 2.3 × 10−12T. These are the same fields which are, due to inhomogeneity, detected as 1,000 times weaker by magnetoencephalography. Considering the widespread occurrence of neurodegenerative disorders, our model reveals that a 50% demyelination increases the field strength by 0.35 × 10−12T, while a complete demyelination increases it by 0.7 × 10−12T. Since this suggests that the inhomogeneous electromagnetic fields around neurons play a role in physiological and pathological processes, including cell-to-neuron and cell-to-cell communication, their improved understanding opens up new therapeutic strategies based on electromagnetic field modulation or cell’s surface charge alteration.

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Stroke promotes survival of nearby transplanted neural stem cells by decreasing their activation of caspase 3 while not affecting their differentiation

Cited by 9 publications

References 35 publications

Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic Murine Model

Melatonin-Pretreated Mesenchymal Stem Cells Improved Cognition in a Diabetic Murine Model

Transplantation of neural stem cells in the mouse model of ischemic brain stroke and expression of genes involved in programmed cell death

Modeling of inhomogeneous electromagnetic fields in the nervous system: a novel paradigm in understanding cell interactions, disease etiology and therapy

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