Stroke‐prone spontaneously hypertensive rats have reduced hydroxysteroid 17‐β dehydrogenase 7 levels for low cholesterol biosynthesis

Matsuoka, Hiroshi; Uchino, Yuka; Choshi, Mayuko; Nakamura, Tetsuya; Michihara, Akihiro

doi:10.1111/1440-1681.13188

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…HSD17B7 has not been largely reported in various diseases. HSD17B7 participated in the cholesterol synthesis pathway during the conversion of lanosterol to sterol [ 27 ]. Studies have shown that high triglycerides and low high-density lipoprotein cholesterol levels are markers of high risk of type 2 diabetes [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats

Wang

Zhai

et al. 2021

Bosn J of Basic Med Sci

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Testicular damage and testosterone secretion disorder are associated with diabetes mellitus. Quercetin, a common flavonoid, has antioxidant, anti-cancer, and blood sugar lowering effects. Therefore, this study aims to investigate the effect of quercetin on the reproductive system of male rats with diabetes in vivo and in vitro and elucidate its mechanism. Streptozotocin (STZ) induction was used to establish a diabetes model in forty male Sprague Dawley (SD) rats, which were subsequently administered with 20 or 50 mg/kg of quercetin. Leydig cells of rat testes were treated by high glucose (HG) followed by 5 or 10 μM quercetin. Two doses of quercetin increased rat body weight and testicular weight, decreased blood glucose,and inhibited oxidative stress. RT-qPCR and Western blotting revealed that quercetin alleviated STZ-induced testicular damage and promoted testosterone synthesis. Both doses of quercetin reduced ROS and MDA levels, and increased SOD level in HG-treated cells. Both, in vivo and in vitro results confirmed that a high dose of quercetin was more effective. MiR-1306-5p was upregulated in testicular tissue of diabetic rats and HG-treated cells. 17β-hydroxysteroid dehydrogenase (HSD17B7) was a target of miR-1306-5p and HSD17B7 was downregulated in STZ-induced rat tissues and HG-treated cells. HSD17B7 overexpression reversed the increase of C/EBP homologous protein (CHOP) and glucose-regulated protein 78 (Grp78) protein levels as well as eIF2α phosphorylation level and promotion of cell apoptosis caused by miR-1306-5p overexpression. Moreover, overexpression of HSD17B7 activated the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) axis in HG-treated cells. In conclusion, quercetin inhibits ER stress and improves testosterone secretion disorder through the miR-1306-5p/HSD17B7 axis in diabetic rats.

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Section: Introductionmentioning

confidence: 99%

Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats

Wang

Zhai

et al. 2021

Bosn J of Basic Med Sci

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“…BMI1 [245], CCL5 [246], GREM1 [247], ANGPTL3 [248], ARG2 [249], MSRA (methionine sulfoxidereductase A) [250], SNCA (synuclein alpha) [251], NOX4 [252], PFKFB2 [253], PDZK1 [254], SUCNR1 [255], LYVE1 [256], AZGP1 [257], ERBB4 [258] and PLAT (plasminogen activator, tissue type) [259] might serve as molecular markers for kidney fibrosis. BMI1 [260], IGF2 [261], IRF7 [262], CCL5 [263], ACTN3 [264], E2F1 [265], PF4 [266], TEAD4 [267], TBX4 [268], GREM1 [269], CYP11B2 [270], WNT3A [124], COMP (cartilage oligomeric matrix protein) [271], FLI1 [272], RAP1B [273], ANGPTL3 [274], CYP3A5 [275], HSD11B2 [276], HMGCS2 [277], AGXT2 [278], SLC22A12 [279], FGF1 [280], CRY1 [281], PPARGC1A [282], SLC19A3 [283], CYP2C8 [284], ACOX2 [285], SLC2A9 [286], MSRA (methionine sulfoxidereductase A) [287], VNN1 [288], EPHX2 [289], CROT (carnitine O-octanoyltransferase) [290], SCNN1B [291], NR4A3 [292], HSD17B7 [293], SLC22A2 [294], AQP2 [295], SLC2A2 [296], EGF (epidermal growth factor) [297], ANGPT1 [298], SLC26A4 [299], KL (klotho) [300], SCNN1G [301], PDZK1 [302], PTPRD (protein tyrosine phosphatase receptor type D) [303], ACE2 [304], FOLH1 [305], SUCNR1 [306], GLCE (glucuronic acid epimerase) [307], AQP3 [308], DPP4 [309], REN (renin) [310], TRPM6 [311], ABCB1 [312], MTTP (microsomal triglyceride transfer protein) [313], CALCRL (calcitonin receptor like receptor) [314], ENPEP (glutamylaminopept...…”

Section: Discussionmentioning

confidence: 99%

Identification of novel prognostic targets in acute kidney injury using bioinformatics and next generation sequencing data analysis

Vastrad,

Vastrad

2024

Preprint

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Acute kidney injury (AKI) is a type of renal disease occurs frequently in hospitalized patients, which may cause abnormal renal function and structure with increase in serum creatinine level with or without reduced urine output. With the incidence of AKI is increasing. However, the molecular mechanisms of AKI have not been elucidated. It is significant to further explore the molecular mechanisms of AKI. We downloaded the GSE139061 next generation sequencing (NGS) dataset from the Gene Expression Omnibus (GEO) database. Limma R bioconductor package was used to screen the differentially expressed genes (DEGs). Then, the enrichment analysis of DEGs in Gene Ontology (GO) function and REACTOME pathways was analyzed by g:Profiler. Next, the protein-protein interaction (PPI) network and modules was constructed and analyzed, and the hub genes were identified. Next, the miRNA-hub gene regulatory network and TF-hub gene regulatory network were built. We also validated the identified hub genes via receiver operating characteristic (ROC) curve analysis. Overall, 956 DEGs were identified, including 478 up regulated and 478 down regulated genes. The enrichment functions and pathways of DEGs involve primary metabolic process, small molecule metabolic process, striated muscle contraction and metabolism. Topological analysis of the PPI network and module revealed that hub genes, including PPP1CC, RPS2, MDFI, BMI1, RPL23A, VCAM1, ALB, SNCA, DPP4 and RPL26L1, might be involved in the development of AKI. miRNA-hub gene and TF-hub gene regulatory networks revealed that miRNAs and TFs including hsa-mir-510-3p, hsa-mir-6086 and mir-146a-5p, MAX and PAX2, might be involved in the development of AKI. Various known and newtherapeutic targets were obtained via network analysis. The results of the current investigation might be beneficial for the diagnosis and treatment of AKI.

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“…It is reported that HSD17β7 in a rat is beneficial to increase TC ( Matsuoka et al, 2020 ). In this gene study, as compared with the control group, HSD17β7 ( Supplementary Table 2 ) statistically decreased (Q ≤ 0.01) in the model group.…”

Section: Discussionmentioning

confidence: 99%

Danggui-Shaoyao-San Improves Gut Microbia Dysbiosis and Hepatic Lipid Homeostasis in Fructose-Fed Rats

Yin

Peng

et al. 2021

Front. Pharmacol.

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Metabolic syndrome (MetS) is a pathological state of many abnormal metabolic sections. These abnormalities are closely related to diabetes, heart pathologies and other vascular diseases. Danggui-Shaoyao-San (DSS) is a traditional Chinese medicine formula that has been used as a therapy for Alzheimer’s disease. DSS has rarely been reported in the application of MetS and its mechanism of how it improves gut microbia dysbiosis and hepatic lipid homeostasis. In this study, three extracts of DSS were obtained using water, 50% methanol in water and methanol as extracting solvents. Their chemical substances were analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass (UPLC-Q/TOF-MS). Pharmacodynamic effect of the extracts were evaluated by comparison of biochemical factors, 16S rRNA sequencing test for gut microbiota analysis, as well as metabonomic and transcriptomic assessments on liver tissues from fructose-fed rats. This study aimed at investigating DSS’s mechanism of regulating blood lipid, anti-inflammation and reducing blood glucose. The results showed that the 50% methanol extract (HME) was more effective. It was worth noting that hydroxysteroid 17β-dehydrogenase 13 (HSD17β13) as a critical element of increasing blood lipid biomarker-triglyceride (TG), was decreased markedly by DSS. The influence from upgraded hydroxysteroid 17β-dehydrogenase 7 (HSD17β7) may be stronger than that from downgraded Lactobacillus in the aspect of regulating back blood lipid biomarker-total cholesterol (TC). The differential down-regulation of tumornecrosis factor alpha (TNF-α) and the significant up-regulation of Akkermansia showed the effective effect of anti-inflammation by DSS. The declining glycine and alanine induced the lowering glucose and lactate. It demonstrated that DSS slowed down the reaction of gluconeogenesis to reduce the blood glucose. The results demonstrated that DSS improved pathological symptoms of MetS and some special biochemical factors in three aspects by better regulating intestinal floras and improving hepatic gene expressions and metabolites.

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Stroke‐prone spontaneously hypertensive rats have reduced hydroxysteroid 17‐β dehydrogenase 7 levels for low cholesterol biosynthesis

Cited by 6 publications

References 33 publications

Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats

Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats

Identification of novel prognostic targets in acute kidney injury using bioinformatics and next generation sequencing data analysis

Danggui-Shaoyao-San Improves Gut Microbia Dysbiosis and Hepatic Lipid Homeostasis in Fructose-Fed Rats

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