Stromal cell-laden 3D hydrogel microwell arrays as tumor microenvironment model for studying stiffness dependent stromal cell-cancer interactions

Yue, Xiaoshan; Nguyen, Trung Dung; Zellmer, Victoria R.; Zhang, Siyuan; Zorlutuna, Pınar

doi:10.1016/j.biomaterials.2018.04.001

Cited by 86 publications

(74 citation statements)

References 50 publications

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“…20,21 Models employing micromolding and spheroid formation in a microwell-array platform permit stromal-tumor cell interactions that can affect both the chemical and mechanical microenvironment to influence cell differentiation and migration. 22,23 As more information is obtained on the role of cell-cell communication during invasion, engineered models must also reflect these interactions. For example, myoepithelial cells surrounding the basement membrane are thought to possess a tumor-suppressor role that can be lost during pre-cancerous neoplasia.…”

Section: Step 1: Invasion and Migrationmentioning

confidence: 99%

Endothelial PAI-1 (Plasminogen Activator Inhibitor-1) Blocks the Intrinsic Pathway of Coagulation, Inducing the Clearance and Degradation of FXIa (Activated Factor XI)

Puy

Ngo

Pang

et al. 2019

ATVB

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While considerable progress has been made in studying genetic and cellular aspects of metastasis with in vitro cell culture and in vivo animal models, the driving mechanisms of each step of metastasis are still relatively unclear due to their complexity. Moreover, little progress has been made in understanding how cellular fitness in one step of the metastatic cascade correlates with ability to survive other subsequent steps. Engineered models incorporate tools such as tailored biomaterials and microfabrication to mimic human disease progression, which when coupled with advanced quantification methods permit comparisons to human patient samples and in vivo studies. Here, we review novel tools and techniques that have been recently developed to dissect key features of the metastatic cascade using primary patient samples and highly representative microenvironments for the purposes of advancing personalized medicine and precision oncology. Although improvements are needed to increase tractability and accessibility while faithfully simulating the in vivo microenvironment, these models are powerful experimental platforms for understanding cancer biology, furthering drug screening, and facilitating development of therapeutics.

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Section: Step 1: Invasion and Migrationmentioning

confidence: 99%

Endothelial PAI-1 (Plasminogen Activator Inhibitor-1) Blocks the Intrinsic Pathway of Coagulation, Inducing the Clearance and Degradation of FXIa (Activated Factor XI)

Puy

Ngo

Pang

et al. 2019

ATVB

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“…37,38 Cells in 3D culture may showed more closely mimic in vivo tumor behavior, and a number of 3D culture systems have been reported that more accurately predict the cellular response compared to 2D culture. 39 Thus, 3D culture model is necessary to more accurate clinical drug screening.…”

Section: In Vitro Cell Cytotoxicity Studymentioning

confidence: 99%

The study of establishment of an in vivo tumor model by three-dimensional cells culture systems methods and evaluation of antitumor effect of biotin-conjugated pullulan acetate nanoparticles

Chen

Wei

Chen

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…As a biocompatible hydrogel, GelMA not only non-cytotoxic and biodegradable to cells but also consists of intrinsic arginine, glycine, and aspartate sequences. Thus, it is a superior material for mimicking extracellular matrix interactions in vitro ( Madl and Heilshorn, 2018 ; Yue et al, 2018 ). To perform cell encapsulation in 3D human embryonic kidney cell culture, we introduced micropatterning by exposing the proper mixture of GelMA and PI solution to UV light, to form covalent cross-links and polymerization.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Characterization of Mentha spicata L. Under Differential Dried-Conditions and Associated Nephrotoxicity Screening of Main Compound With Organ-on-a-Chip

Tian

2018

Front. Pharmacol.

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Spearmint (Mentha spicata L.) is normally used as a vegetable flavoring herb. It also has several pharmacological activities against fever, cough, infection, and inflammation. The current study presents an untargeted comparative metabolomics approach utilizing HPLC-QTOF-MS high-throughput analytical technology to provide insights into the effect of the drying process on the examined spearmint species. To the best of our knowledge, this is the first report of compositional differences among fresh and dried spearmint leaves determined via a metabolomic approach to reveal that dried leaves are a better source of bioactive metabolites. The nephrotoxicity of kaempferol, a bioactive metabolite from spearmint, was further assessed with a kidney-on-a-chip. On the designed chip, a GelMA-based 3D culture platform mimics the microenvironment and basic functions of the kidney. In addition, the chip’s transparency allows for direct observation under an optical microscope. Treatment of human embryonic kidney cells with 30 μM of kaempferol for 12 h induced no obvious cell injury or apoptosis in the cells, on the basis of morphology, thus providing a proof-of-concept demonstration of kaempferol’s non-toxicity.

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Stromal cell-laden 3D hydrogel microwell arrays as tumor microenvironment model for studying stiffness dependent stromal cell-cancer interactions

Cited by 86 publications

References 50 publications

Endothelial PAI-1 (Plasminogen Activator Inhibitor-1) Blocks the Intrinsic Pathway of Coagulation, Inducing the Clearance and Degradation of FXIa (Activated Factor XI)

Endothelial PAI-1 (Plasminogen Activator Inhibitor-1) Blocks the Intrinsic Pathway of Coagulation, Inducing the Clearance and Degradation of FXIa (Activated Factor XI)

The study of establishment of an in vivo tumor model by three-dimensional cells culture systems methods and evaluation of antitumor effect of biotin-conjugated pullulan acetate nanoparticles

Phytochemical Characterization of Mentha spicata L. Under Differential Dried-Conditions and Associated Nephrotoxicity Screening of Main Compound With Organ-on-a-Chip

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