Stromal cells of the human prostate: Initial isolation and characterization

Kassen, Annette; Sutkowski, Debra M.; Ahn, Hanjong; Sensibar, Julia A.; Kozlowski, James M.; Lee, Chung

doi:10.1002/(sici)1097-0045(199602)28:2<89::aid-pros3>3.0.co;2-i

Cited by 99 publications

(59 citation statements)

References 19 publications

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“…Neither DHT, T, DHEA, nor E 2 significantly affected IGF-I receptor, IGF-II, or IGFBP-5 mRNA levels or stromal cell proliferation. Previous studies have reported a 30 -50% increase in cell growth after T and DHT administration to prostate stromal cells derived from BPH tissue (24,28); however, the effects of androgens or estrogens on cell growth of stromal cells derived from normal or malignant prostate tissue have not been clearly defined.…”

Section: Discussionmentioning

confidence: 95%

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

Le¹,

Arnold

McFann

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

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Prostate cancer is one of the four most common cancers in the United States, affecting one of six men. Increased serum levels of androgens and IGF-I are associated with an augmented risk of prostate cancer. Dihydrotestosterone (DHT) and testosterone (T) stimulate prostate cancer cell growth, development, and function, whereas the effects of DHT and T in prostate stromal cells, and of dehydroepiandrosterone (DHEA) in prostate cancer or stromal cells, are uncertain. We investigated the actions of DHT, T, DHEA, and estradiol (E2) on insulinlike growth factor (IGF)-I, IGF-II, IGF-I receptor (R), IGF-binding protein (IGFBP)-2, IGFBP-3, and IGFBP-5 in primary cultures of human prostatic stromal cells by assessing cell proliferation, mRNA expression, and protein secretion by MTT growth assay, quantitative real-time PCR, and ELISA, respectively. DHT and T each increased IGF-I (7-fold) and decreased IGFBP-3 (2-fold) mRNA expression and protein secretion in a dose-and time-dependent manner and increased IGFBP-2 (2-fold) mRNA in a dose-and time-dependent manner. DHEA and E 2 did not significantly alter these measures. Flutamide abolished the DHT-modulated increases in IGF-I and IGFBP-2, suggesting that the influences of DHT and T on these measures were androgen receptor mediated. None of the four steroids significantly affected IGF-IR, IGF-II, or IGFBP-5 mRNA levels or stromal cell proliferation. The effects of DHT on IGF-I, IGFBP-2, and IGFBP-3 were more pronounced in stromal cultures that did not express desmin. These data suggest that DHT and T promote prostate growth partly via modulation of the stromal cell IGF axis, with potential paracrine effects on prostate epithelial cells.

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Section: Discussionmentioning

confidence: 95%

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

Le¹,

Arnold

McFann

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

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“…TGF-ß1 is produced by basal cells that also express both corresponding receptors (16). While low concentrations support proliferation, high TGF-ß1 concentrations have been shown to inhibit prostate stromal cell growth (17). Prostatic fibroblasts undergo transdifferentiation into myofibroblasts/smooth muscle cells (SMCs) upon stimulation with TGF-ß1 (18,19).…”

Section: Discussionmentioning

confidence: 99%

Characteristic gene expression profiles of benign prostatic hypertrophy and prostate cancer

Endo

Uzawa²,

Suzuki³

et al. 2009

Int J Oncol

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Abstract. The molecular mechanism playing a role in the development of benign prostate hypertrophy (BPH) and prostate cancer (PC) is not well defined. We performed microarray analysis to assess the gene expression change in BPH and PC, and performed network analysis. Normal prostate, BPH and PC tissues were obtained from patients who underwent an operation at Chiba University Hospital. Using Affymetrix Human Genome U133 Plus2.0 Array, we identified genes differentially expressed. The identified genes were analyzed using the Ingenuity Pathway Analysis (IPA) to investigate the functional network and gene ontology. The microarray analysis identified 402 genes in BPH and 141 genes in PC, which were up-or down-regulated at least 5.0-fold change in PC at all dose points. Analysis using IPA software revealed eight networks in BPH and five networks in PC. We narrowed these down to the top five genes, which were up-or down-regulated on the networks in their characteristic manner. From this new perspective, comparing BPH and PC in microarray studies, our data showing gene expression profiles provide candidate genes for better understanding of disease and new therapeutic targets.

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“…Ten milliliters of 5% fetal bovine serum-RPMI 1640 medium (BioWhittaker) supplemented with gentamicin and fungicide was added to 1-2 g of minced tissue. Powder collagenase type I (GIBCO͞BRL) was added, and the mixture was digested overnight at 37°C with gentle stirring (13). The suspension was passed the next day through a 70-m mesh nylon filter (Falcon 2350, Becton Dickinson) and then aspirated with a 23-gauge needle.…”

Section: Methodsmentioning

confidence: 99%

Cell–cell interaction in prostate gene regulation and cytodifferentiation

Liu

True

LaTray

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

180

154

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Stromal cells of the human prostate: Initial isolation and characterization

Cited by 99 publications

References 19 publications

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

Characteristic gene expression profiles of benign prostatic hypertrophy and prostate cancer

Cell–cell interaction in prostate gene regulation and cytodifferentiation

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Stromal cells of the human prostate: Initial isolation and characterization

Cited by 99 publications

References 19 publications

DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

Characteristic gene expression profiles of benign prostatic hypertrophy and prostate cancer

Cell–cell interaction in prostate gene regulation and cytodifferentiation

Contact Info

Product

Resources

About

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells

DHT and testosterone, but not DHEA or E₂, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells