Stromal–epithelial measurements of prostate cancer in native Japanese and Japanese-American men

Abstract

We measured the histologic stromal and epithelial tissue components of the benign (normal) and malignant tissue compartments of Japanese-Americans (J-A) and native Japanese (NJ) men living in Japan. The patient cohort included 25 NJ men undergoing radical prostatectomy (RP) in Nagoya, Japan and 25 J-A (second or third generation US born). We conducted tissue image quantitation (in-house image software) of the stromal and epithelial compartments in malignant and adjacent normal tissue areas from a tissue microarray (TMA) selected from radical prostatectomy (RP) blocks. Stromal-epithelial (S-E) areas were determined using immunohistochemical stains for CAM-5.2 epithelial cytokeratin marker and the Masson trichrome stain to measure the stroma component. We observed differences in the volumes of normal and cancer epithelium and stroma within both the J-A and NJ study populations (Po0.01). Only the individual average cancer epithelium (CE) volume (JA ¼ 24.1 vs NJ ¼ 29.9) differed significantly between the NJ and J-A study populations (P ¼ 0.03). Consequently, the S-E ratio in NJ group was significantly different from that of J-A population (P ¼ 0.05). The decrease in S-E ratio noted in the malignant tissues of NJ prostate tissue may provide a biological marker for differentiation of the two groups and suggests a need for further investigations into the molecular basis for these histologic differences.

Epithelium percentage estimation facilitates epithelial quantitative protein measurement in tissue specimens

“…The average epithelium percentage in normal prostate tissues was 24.14 ± 5.58% (Figure 5A). This ratio was similar to what was reported before [3]. The average epithelium percentage in prostate tumors was 57.98 ± 19.75%.…”

“…Traditionally, the epithelium contents are read based on nuclei counts from a hematoxylin and eosin (H&E) stained histology slide by a pathologist. Another approach is to stain the histology slide with anti-cytokeratin antibody CAM 5.2 (staining for epithelia) and Masson trichrome (staining for collagenous stromal structures) [3]. More recently, with the digitization of whole slide imaging, a number of algorithms have been developed for computer-assisted readings.…”

Histopathological differences between prostate cancer foci that are detected and missed using multiparametric magnetic resonance imaging in Korean patients

“…Several studies have examined the effect of racial differences on BPH and PCa cellular composition. 19,20 In the present study, no histopathological differences were observed between MRS-detected and -undetected PCa foci, and we found that the (Cho + Cr)/Cit ratio was not correlated with any histopathological features, although the mean (Cho + Cr)/ Cit ratio was significantly higher for detected PCa foci than for undetected PCa foci. A large, recent prospective multicenter study showed no benefit of spectroscopy to localize PCa compared with T2-weighted imaging alone.…”

“…These different results might have originated from the effect of racial differences, lifestyle or environmental factors. Several studies have examined the effect of racial differences on BPH and PCa cellular composition …”

Hubungan Persentase Dan Pola Stroma Reaktif Dengan Derajat Histopatologi Lesi Prostat

“…Stroma bukan hanya sebagai penyokong neoplasma epitelial yang diam, namun juga berperan secara aktif dan dinamis dalam tumorigenesis dan progresi kanker prostat. (7) Dari penelitian Chung, seperti yang ditulis oleh Veltri et al, 2004, (7) diajukan bahwa fibroblast yang tumorigenik yang diinduksi oleh proliferasi epitel, kemudian diorganisasi ke dalam bentuk adenokarsinoma melalui interaksi sel dengan sel. Fibroblast yang tumorigenik ini juga menunjukkan efek penguatan pada sel karsinoma prostat pada manusia dan tikus.…”