Stromal Expression of CD10 by Immunohistochemistry in Odontogenic Keratocyst (OKC), Dentigerous and Radicular Cysts and Its Correlation with Local Recurrence and Aggressive Behaviour

Abstract

Objective:To assess and compare the stromal expression of CD10 in OKC, dentigerous and radicular cysts. Materials and Methods: This comparative, cross sectional study was conducted at Armed Forces Institute of Pathology (AFIP), Rawalpindi, from Jan 2017 to Dec 2017. Total sixty cases comprising 20 of each OKC, Dentigerous and Radicular cysts were included in this study. Hematoxylin and eosin (H and E) sections were performed followed by immunohistochemical staining for CD10 antibody. Expression of CD10 was evaluated and compared. Results were analyzed by using SPSS version 20.0. Chi Square test was performed with P value < 0.05 was considered as significant. Results: A total of 60 cases, 20 of each OKC, dentigerous and radicular cysts were taken. In our study, 38 (63.3%) male and 22 (36.7%) female patients with the mean age of 32 ± 15 (mean ± SD) were included. Percentage of CD10 positive cells were highest in sub-epithelial stroma of OKC (95% cases) as compared to radicular and dentigerous cysts (60 and 70%) with highest number of cases showing intense staining in OKC 13(65%) as compared to other odontogenic cysts i-e 4(20%) and 2 (10%) respectively. There was a statistically significant association between odontogenic cysts and proportional score, intensity score and combined score of stromal CD10 expression (P=0.009, p=0.001 and p=0.000). Conclusion: In this study, we found that highest stromal CD10 expression in OKC as compared to dentigerous and radicular cyst, which might be due to aggressive behaviour and increased risk of recurrence in OKC. Expression of CD10 marker will further aid the clinician to plan appropriate surgical intervention and keep regular follow-ups to identify recurrences.

The expression of BMI-1 in ameloblastoma and dentigerous cyst

“…16,20 This reflects the low activity in the proliferative component of the lining and consequently the known indolent course of DC. 3,5,16,21,22 An original finding in our research is the dual staining pattern, nuclear and cytoplasmic, with a predominance of the former pattern in the Am group of neoplastic nature. The cytoplasmic immunoreaction in the current study is justified by the study which proved the presence of BMI-1 in the inner mitochondrial membrane.…”

Role of matrix metalloproteinases and their tissue inhibitors in the pathological mechanisms underlying maxillofacial cystic lesions

“…The comparisons of MMP activity in the cysts and in other odontogenic tumors is understandable, given their more aggressive potential. Ameloblastoma, which is described as a benign, locally aggressive odontogenic tumor, and odontogenic keratocyst (OKC) are characterized by higher destructive potentials and a tendency to recur when compared with RCs and DCs (7). According to the World Health Organizations recommendations on the reclassification of keratocystic odontogenic tumors (KCOTs) into the group of OKCs, all references to KCOT in the present study should be considered as an OKC (30).…”

“…Numerous studies have been conducted to investigate the mechanisms involved in both types of lesions. MMPs are a group of proteins potentially involved in cyst formation and bone destruction (7)(8)(9)(10)(11). This family of zinc-dependent enzymes is responsible for the degradation of the extracellular matrix (ECM) during various physiological and pathological processes, including in embryogenesis, organization of dentin organic matrix, arthritis and ischemic stroke (12,13).…”

CD10 and CD34 as markers in vascular malformations with PIK3CA and TEK mutations

“…The rare discrepancies in interpreting the results were checked by a third investigator (G.B.). CD10 and CD34 expression were evaluated separately in the endothelial cells of malformed vessels and in mesenchymal cells embedding the vMs, employing a score combining staining intensity and percentage according to Anam Ali [19] (Table 1 and Figure 1).…”