Stromal expression of JNK1 and VDR is associated with the prognosis of esophageal squamous cell carcinoma

Bao, Yonghua; Zhang, S.; Guo, Yanning; Wei, Xin; Zhang, Y.; Yang, Yuyao; Zhang, H.; Ma, Ming; Yang, Wancai

doi:10.1007/s12094-018-1843-2

Cited by 17 publications

(21 citation statements)

References 40 publications

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“…Vitamin D enters cells by two pathways: the steroid receptor pathway and the direct entry pathway. Vitamin D receptor (VDR) is a membrane receptor for vitamin D. However, whether VDR is a tumour suppressor or an oncogene remains unclear [27][28][29] . The VDR expressions in cisplatin and vitamin D treatments failed to show significant changes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Huang

Zhang

et al. 2019

Cell Death Dis

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Chemoresistance is a major cause of cancer progression and the mortality of cancer patients. Developing a safe strategy for enhancing chemosensitivity is a challenge for biomedical science. Recent studies have suggested that vitamin D supplementation may decrease the risk of many cancers. However, the role of vitamin D in chemotherapy remains unknown. We found that vitamin D sensitised oral cancer cells to cisplatin and partially reversed cisplatin resistance. Using RNA-seq, we discovered that lipocalin 2 (LCN2) is an important mediator. Cisplatin enhanced the expression of LCN2 by decreasing methylation at the promoter, whereas vitamin D enhanced methylation and thereby inhibited the expression of LCN2. Overexpression of LCN2 increased cell survival and cisplatin resistance both in vitro and in vivo. High LCN2 expression was positively associated with differentiation, lymph node metastasis, and T staging and predicted a poor prognosis in oral squamous cell carcinoma (OSCC) patients. LCN2 was also associated with post-chemotherapy recurrence. Moreover, we found that LCN2 promoted the activation of NF-κB by binding to ribosomal protein S3 (RPS3) and enhanced the interaction between RPS3 and p65. Our study reveals that vitamin D can enhance cisplatin chemotherapy and suggests that vitamin D should be supplied during chemotherapy; however, more follow-up clinical studies are needed.

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Section: Discussionmentioning

confidence: 99%

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Huang

Zhang

et al. 2019

Cell Death Dis

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“…He et al showed that activating MAPK8 could induce autophagy and reduce apoptosis of cancer cells [34]. Bao et al found low expression of MAPK8 in ESCA, and showed that overexpression of MAPK8 could inhibit the proliferation and migration of cancer cells and was related to poor prognosis in ESCA patients [35]. CAPN10 is highly expressed in ESCA.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of autophagy-related lncRNAs in esophageal carcinoma

Ding

Fan

2020

Preprint

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Background: Esophageal carcinoma (ESCA) is a malignant tumor with high invasiveness and mortality. Autophagy has multiple roles in the development of cancer; however, there are limited data on autophagy genes associated with long non-coding RNAs (lncRNAs) in ESCA. The purpose of this study was to screen potential diagnostic and prognostic molecules, and to identify gene co-expression networks associated with autophagy in ESCA. Methods: We downloaded transcriptome expression profiles from The Cancer Genome Atlas and autophagy-related gene data from the Human Autophagy Database, and analyzed the co-expression of mRNAs and lncRNAs. In addition, the diagnostic and prognostic value of autophagy-related lncRNAs was analyzed by multivariate Cox regression. Furthermore, Kyoto Encyclopedia of Genes and Genomes analysis was carried out for high-risk patients, and enriched pathways were analyzed by gene set enrichment analysis. Results: The results showed that genes of high-risk patients were enriched in protein export and spliceosome. Based on Cox stepwise regression and survival analysis, we identified seven autophagy-related lncRNAs with prognostic and diagnostic value, with the potential to be used as a combination to predict the prognosis of patients with ESCA. Finally, a co-expression network related to autophagy was constructed. Conclusion: These results suggest that autophagy-related lncRNAs and the spliceosome play important parts in the pathogenesis of ESCA. Our findings provide new insight into the molecular mechanism of ESCA and suggest a new method for improving its treatment.

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“…Total RNA was extracted from endometrial cancer and nonneoplastic endometrial tissues using TRIzol RNA extraction agent (Invitrogen) according to the manufacturer's instructions 12. Only RNA that resulted in an A260/280 ratio of 1.8-2.0 was reverse transcribed to generate cDNA.…”

Section: Methodsmentioning

confidence: 99%

The voltage-gated sodium channel Na_v1.7 associated with endometrial cancer

Liu¹,

Tan²,

Yang³

et al. 2019

J. Cancer

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Background: Endometrial cancer is the most common gynecologic malignancy in women in the developed countries. Despite recent progress in functional characterization of voltage-gated sodium channel (Na v) in multiple cancers, very little was known about the expression of Na v in human endometrial cancer. The present study sought to determine the role of Na v and molecular nature of this channel in the endometrial cancer. Methods: PCR approach was introduced to determine expression level of Na v subunits in endometrial cancer specimens. Pharmacological agents were used to investigate Na v function in endometrial cancer cells. Flow cytometry were used to test cancer apoptosis, and invasion assays were applied to test tumor metastasis. Results: Transcriptional levels of the all Na v α and β subunits were determined by real time-PCR in endometrial cancer with pair tissues of carcinoma and adjacent nonneoplastic tissue, Na v 1.7 was the most highly expressed Na v subtype in endometrial cancer tissues. Na v 1.7 level was closely associated with tumor size, local lymph node metastasis, and 5-year and 10-year survival ratio. Inhibition of this channel by Na v 1.7 blocker PF-05089771, promoted cancer apoptosis and attenuated cancer cell invasion. Conclusion: These results establish a relationship between voltage-gated sodium channel protein and endometrial cancer, and suggest that Na v 1.7 is a potential prognostic biomarker and could serve as a novel therapeutic target for endometrial cancer.

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Stromal expression of JNK1 and VDR is associated with the prognosis of esophageal squamous cell carcinoma

Abstract: JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma.

Cited by 17 publications

References 40 publications

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Bioinformatics analysis of autophagy-related lncRNAs in esophageal carcinoma

The voltage-gated sodium channel Na_v1.7 associated with endometrial cancer

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Stromal expression of JNK1 and VDR is associated with the prognosis of esophageal squamous cell carcinoma

Abstract: JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma.

Cited by 17 publications

References 40 publications

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Vitamin D promotes the cisplatin sensitivity of oral squamous cell carcinoma by inhibiting LCN2-modulated NF-κB pathway activation through RPS3

Bioinformatics analysis of autophagy-related lncRNAs in esophageal carcinoma

The voltage-gated sodium channel Nav1.7 associated with endometrial cancer

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The voltage-gated sodium channel Na_v1.7 associated with endometrial cancer