Strong Delayed-Type Hypersensitivity Induced against House Dust Mite Antigens in the Mice

Nakano, Yumiko; Yoshida, M.; Shibata, Tetsuo

doi:10.1159/000234729

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…For the induction and elicitation of delayed‐type hypersensitivity we used the method of Nakano et al (23). Briefly, 8 to 10 week‐old NC/Kuj mice were first injected i.p.…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) on Chronic Dermatitis

Miyoshi

Kanekura

Aoki³

et al. 2005

The Journal of Dermatology

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Chronic dermatitis, such as contact dermatitis (CD) or atopic dermatitis (AD), is a longstanding inflammatory skin disease with cutaneous damage such as erosion, ulceration, and lichenification due to itch-induced scratching. The resultant lesion can be considered to be a kind of wound. The tissue inhibitor metalloproteases-2 (TIMP-2) accelerates wound healing by enhancing the proliferation and migration of epidermal keratinocytes and dermal fibroblasts; it is also a physiologic inhibitor of matrix metalloproteinases. The aim of this study was to test the effect of TIMP-2 on chronic dermatitis. NC/Kuj mice were sensitized with Dermatophagoides farinae (Df) extract. Eczema was induced by repeated applications of this mite allergen to the skin of 20 sensitized mice that were maintained under specific pathogen-free conditions. One group of 10 mice was then treated with topical TIMP-2 solution (0.1 ml, 0.5%) for 28 days, and the other with vehicle alone and the effects of TIMP-2 were evaluated macro- and microscopically. The effect on skin barrier function was estimated by measuring transepidermal water loss (TEWL). Scoring of gross skin findings showed that TIMP-2 significantly reduced the severity of eczema (P<0.05) on days 12-28. Histological examination revealed that TIMP-2 treated mice manifested lower degrees of hyperkeratosis, acanthosis, and spongiosis in the epidermis and fewer inflammatory cells in the dermis than vehicle-treated mice. There were significant reductions in the epidermal thickness and dermal inflammatory cells in the TIMP-2 treated animals (P<0.01); their TEWL was significantly decreased on day 28 (P<0.05). Our results suggest that NC/Kuj mice with Df extract-induced chronic eczema may be a useful model for investigating chronic dermatitis, and that TIMP-2 may be a good agent for treating chronic dermatitis as well as chronic ulcers.

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“…For the induction and elicitation of delayed‐type hypersensitivity we used the method of Nakano et al (23). Briefly, 8 to 10 week‐old NC/Kuj mice were first injected i.p.…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) on Chronic Dermatitis

Miyoshi

Kanekura

Aoki³

et al. 2005

The Journal of Dermatology

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Delayed-type hypersensitivity to storage mites of theTyrophagus putrescentiae complex in experimental allergic dermatosis

Ado¹,

Mokronosova²,

Zheltikova³

et al. 1992

Bull Exp Biol Med

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The relevance of murine animal models to study the development of allergic bronchial asthma

Herz

Lumpp

Palma

et al. 1996

Immunology & Cell Biology

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Summary Bronchial asthma (BA) develops on the basis ofa genetic predisposition and involves a characteristic sequence of changes in immune functions. In the immunopathogcncsis. several phases can be distinguished: the initial stage is defined as the development of allergic sensitization. This step is dependent on: (i) T cell activation; (ii) IL-4 production; (iii) IgE synthesis; and (iv) mediator release by effector cells. The second phase of allergic inflammation as a consequence ofthe T cell dependent sensitization is characterized by IL-5 production and eosinophil activation and recruitment. Airway mueosa remodelling is the consequence of chronic inflammatory processes and represents the final stage of BA. In this article animal models will be discussed with regard to their relevance for these different phases in development of chronic allergic BA.

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Strong Delayed-Type Hypersensitivity Induced against House Dust Mite Antigens in the Mice

Cited by 4 publications

References 9 publications

Beneficial Effects of Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) on Chronic Dermatitis

Beneficial Effects of Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) on Chronic Dermatitis

Delayed-type hypersensitivity to storage mites of theTyrophagus putrescentiae complex in experimental allergic dermatosis

The relevance of murine animal models to study the development of allergic bronchial asthma

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