The ictal EEG biomarkers of the epileptogenic zone (EZ) need to be better defined. The power and structure of ictal fast activity are important in EZ localization, but EEG onset patterns are heterogeneous and initial fast activity is absent in many patients. Here we defined a unique spectral structure of “harmonic pattern” (H pattern) on stereo-EEG (SEEG), characterized by multiple equidistant, high-density bands with varying frequency on time frequency map. H pattern was commonly observed among 57 (81.4%) out of 70 patients with focal onset pattern on SEEG. It was presented in seizures with various ictal onset patterns with or without fast activity, and during early or late stage of seizures. H pattern usually expressed at very close time point across the seizure onset zone (SOZ), primary propagation zone and sometimes other areas, with the same fundamental difference, reflecting an inter-regional synchronization within the ictal network during this time. Notably, SOZ showed the highest proportion of channels expressing H-pattern, and also highest band number of H-pattern. At patient level, the dominant H pattern was defined as those with high rank in band numbers (the third quartile, Q3). Resection of the region expressing dominant H pattern, but not SOZ, independently predicted seizure freedom after surgery, suggesting it is an ictal marker of EZ. How H pattern was produced was then investigated. It only embedded into two types of EEG segments: fast activity with a frequency >25Hz (FA-H pattern) at early seizure propagation (mean 13.3 sec after onset), and irregular polyspikes (> 5 Hz, PS-H pattern) during late propagation (mean 23.3 sec after onset). Nonlinear analysis was used to unravel the mechanism underlying H pattern generation. Our data showed it was produced by specific nonlinear phenomena rather than intermodulation of frequencies or purely methodological artefact. The nonlinearity was stronger for dominant compared to non-dominant H pattern. According to the spectral parameters, we postulate that FA-H pattern may be supported by a predominant and synchronized firing of GABAergic neurons, while excitatory neuron firing is more important for PS-H pattern. As a distinctive and common ictal spectral feature, H pattern conveys unique information of ictal neural dynamics and provides new insights into the EZ. Our study also provides evidence that there is an elongated time-window to measure EZ using quantitative EEG.