Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon‐specific primers

Tedersoo, Leho; Jairus, Teele; Horton, Bryony M.; Abarenkov, Kessy; Suvi, Triin; Saar, Irja; Kõljalg, Urmas

doi:10.1111/j.1469-8137.2008.02561.x

Cited by 372 publications

(303 citation statements)

References 63 publications

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“…Moreover, the significance of host effects would depend on spatial scales and host composition within a stand. Our stand-scale analyses detected significant host effects at some sites, especially in gymnosperm-angiosperm mixed forests (Miyamoto,unpublished data;Supplementary Table S3), as in many previous studies (Ishida et al, 2007;Tedersoo et al, 2008;Smith et al, 2009). However, most EM fungi are assumed to be host generalists (Bruns et al, 2002), which is supported by numerous studies (Kennedy et al, 2003;Roy et al, 2008;Trocha et al, 2012;Bahram et al, 2013b).…”

Section: Discussionsupporting

confidence: 68%

“…The host plant is regarded as one of the most important factors that influences EM fungal composition, especially at the stand scale, where hosts coexist in relatively narrow spatial and environmental ranges (Kennedy et al, 2003;Richard et al, 2005;Ishida et al, 2007;Tedersoo et al, 2008;Smith et al, 2009;Murata et al, 2013). However, isolating the host effect at larger spatial scales is difficult because the host composition changes with climatic conditions and geological history .…”

Section: Introductionmentioning

confidence: 99%

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Strong effect of climate on ectomycorrhizal fungal composition: evidence from range overlap between two mountains

et al. 2015

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Separating the effects of environmental factors and spatial distance on microbial composition is difficult when these factors covary. We examined the composition of ectomycorrhizal (EM) fungi along elevation gradients on geographically distant mountains to clarify the effect of climate at the regional scale. Soil cores were collected from various forest types along an elevation gradient in southwestern Japan. Fungal species were identified by the internal transcribed spacer regions of the rDNA using direct sequencing. The occurrence of fungal species in this study was compared with a previous study conducted on a mountain separated by B550 km. In total, we recorded 454 EM fungi from 330 of 350 soil cores. Forty-seven fungal species (B20% of the total excluding singletons) were shared between two mountains, mostly between similar forest types on both mountains. Variation partitioning in redundancy analysis revealed that climate explained the largest variance in EM fungal composition. The similarity of forest tree composition, which is usually determined by climatic conditions, was positively correlated with the similarity of the EM fungal composition. However, the lack of large host effects implied that communities of forest trees and EM fungi may be determined independently by climate. Our data provide important insights that host plants and mutualistic fungi may respond to climate change idiosyncratically, potentially altering carbon and nutrient cycles in relation to the plant-fungus associations.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Strong effect of climate on ectomycorrhizal fungal composition: evidence from range overlap between two mountains

et al. 2015

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“…In the past two decades, analysis of DNA sequences has been incorporated to establish phylogenetic relationships among groups and to aid species delimitation [8][9][10][11][12]. Nowadays, efforts have been made to seek a short, standardized, and universal gene marker for rapid species identification of diverse groups of fungi [5,[13][14][15][16][17][18][19]. So far, a single universal DNA barcode for fungi has not been obtained [20].…”

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confidence: 99%

DNA barcoding of the fungal genus Neonectria and the discovery of two new species

Zhao

Luo

Zhuang

et al. 2011

Sci. China Life Sci.

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To determine a suitable DNA barcode for the genus Neonectria, the internal transcribed spacer rDNA, β-tubulin, EF-1α, and RPB2 genes were selected as candidate markers. A total of 205 sequences from 19 species of the genus were analyzed. Intraand inter-specific divergences and the ease of nucleotide sequence acquisition were treated as criteria to evaluate the feasibility of a DNA barcode. Our results indicated that any single gene among the candidate markers failed to serve as a successful barcode, while the combination of the partial EF-1α and RPB2 genes recognized all species tested. We tentatively propose the combined partial EF-1α and RPB2 genes as a DNA barcode for the genus. During this study, two cryptic species were discovered, based on the combined data of morphology and DNA barcode information. We described and named these two new species N. ditissimopsis and N. microconidia. intra-specific variation, inter-specific variation, morphology, DNA barcode, sequence analysis Citation:Zhao P, Luo J, Zhuang W Y, et al. DNA barcoding of the fungal genus Neonectria and the discovery of two new species . Sci China Life Sci, 2011, 54: 664 -674,

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“…28S rRNA gene amplification used concatemers containing the fungal-specific LROR (Tedersoo et al, 2008), an 8-bp multiplex tag, and the 454 'B' adaptor (in the forward direction) and the complimentary primer LR5F (Tedersoo et al, 2008) with the 454 'A' adaptor (in the reverse direction). Following an initial denaturation step at 95°C for 3 min, PCR was cycled 35 times at 95°C for 30 s, 54°C for 45 s, 72°C for 50 s, with a final extension at 72°C for 10 min.…”

Section: Microbial Community Sequencingmentioning

confidence: 99%

Temporal variation overshadows the response of leaf litter microbial communities to simulated global change

et al. 2015

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Bacteria and fungi drive the decomposition of dead plant biomass (litter), an important step in the terrestrial carbon cycle. Here we investigate the sensitivity of litter microbial communities to simulated global change (drought and nitrogen addition) in a California annual grassland. Using 16S and 28S rDNA amplicon pyrosequencing, we quantify the response of the bacterial and fungal communities to the treatments and compare these results to background, temporal (seasonal and interannual) variability of the communities. We found that the drought and nitrogen treatments both had significant effects on microbial community composition, explaining 2-6% of total compositional variation. However, microbial composition was even more strongly influenced by seasonal and annual variation (explaining 14-39%). The response of microbial composition to drought varied by season, while the effect of the nitrogen addition treatment was constant through time. These compositional responses were similar in magnitude to those seen in microbial enzyme activities and the surrounding plant community, but did not correspond to a consistent effect on leaf litter decomposition rate. Overall, these patterns indicate that, in this ecosystem, temporal variability in the composition of leaf litter microorganisms largely surpasses that expected in a short-term global change experiment. Thus, as for plant communities, future microbial communities will likely be determined by the interplay between rapid, local background variability and slower, global changes.

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Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon‐specific primers

Cited by 372 publications

References 63 publications

Strong effect of climate on ectomycorrhizal fungal composition: evidence from range overlap between two mountains

Strong effect of climate on ectomycorrhizal fungal composition: evidence from range overlap between two mountains

DNA barcoding of the fungal genus Neonectria and the discovery of two new species

Temporal variation overshadows the response of leaf litter microbial communities to simulated global change

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