Strong Stimulation of CBA Lymphocytes in the Mixed Lymphocyte Interaction with Cells from the H‐2‐Identical Strain C3H

Abstract

Lymphocyt e! from the H‐2‐identical mouse strains CBA and C3H were tested for mixed lymphocyte culture (MLC) responsiveness in vitro. It was observed that CBA lymphocytes were stimulated by C3H cells to the same extent as or stronger than cells from H‐2‐disparate donors. C3H lymphocytes, however, were stimulated poorly by CBA cells. The stimulatory effect by C3Hcells of CBA lymphocytes was observed to be positively correlated with the age of the C3H donor. A similar but less pronounced difference was found by varying the age of donors for H‐2 disparate cells. Spleen cells from normal and thymectomized, irradiated, and bone marrow‐reconstituted C3H × CBA mice stimulated CBA lymphocytes to approximately the same extent, indicating that the stimulatory antigen is present on both T and B cells or possibly other cell types However, they arc poorly expressed or absent on thymic cells. It is concluded that certain non‐H‐2‐determined transplantation antigens may be strongly stimulatory in MLC tests.

Activated Mouse Lymphocyte Release Factors Which Modulate the Proliferative Responses of Other Lymphocytes

“…Unless otherwise stated, each culture received 1.0 «Ci of 3H-thymidine after 48 h of incubation in a humidi fied 5°/o C 0 2-air atmosphere (specific activity 5 Ci/ mA/, The Radiochemical Center, Amersham, England). 16 h later, the cultures were terminated and incorporated radioactivity determined as de scribed before [7], Arithmetic mean values of du plicate cultures were calculated and expressed as counts per minute (cpm).…”

“…A microculture technique was employed which has been described in detail before [7], In short, lymphocytes obtained from thymi and lymph nodes of 1-to 2-month-old CBA mice were cul tured in the wells of microtest plates containing 0.2 ml of active or control supernatant. In one type of experiment, some of the cultures received phytomitogens at final concentrations indicated in the text, whereas others without phytomitogens served as controls.…”

Social Support and Immune Status During and after Chemotherapy for Breast Cancer

“…The cells were stimulated with this agent at concentrations of 75, 37.5, and 9.4 pg/ml. A microculture technique was used which has been described earlier (34). In brief, lo5 non-purified lymphocytes (contaminated with approximately 15% monocytes) were cultured with or without mitogens in 0.2 ml of RPMI 1640 medium, supple- Note.…”

Blood Lymphocyte Population 4 to 6 Years after Adjuvant Cyclic Chemotherapy for Operable Breast Carcinoma

“…Accepted for publication 11 May 1984. Eleven of the patients had received LMF treatments which were completed 60 to 76 months before testing and 10 of the patients had received CMF treatments 47 to 59 months earlier. Two of the latter patients had also been treated with Nolvadex (Tamoxifen) during a 2-year period.…”