Strongyloides genotyping: a review of methods and application in public health and population genetics

Bradbury, Richard S.; Pafčo, Barbora; Nosková, Eva; Hasegawa, Hideo

doi:10.1016/j.ijpara.2021.10.001

Cited by 23 publications

(38 citation statements)

References 65 publications

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“…ceylanicum is a zoonoses and is a predominant hookworm of domestic dogs and cats in the Asia Pacific region [ 35 ]. Strongyloides spp., for which WHO recently introduced control targets to be attained by 2030 [ 5 ], is also a potential zoonosis comprising two distinct genetic clades, one restricted to dogs and another infecting humans, non-human primates, dogs and cats [ 7 , 36 ]. Zoonotic transmission therefore needs to be addressed through One Health strategies.…”

Section: Discussionmentioning

confidence: 99%

Using quantitative PCR to identify opportunities to strengthen soil-transmitted helminth control in Solomon Islands: A cross-sectional epidemiological survey

Clarke

Hii

et al. 2022

PLoS Negl Trop Dis

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Background The Kato-Katz microscopy technique is the global standard for assessment of soil-transmitted helminth (STH) burden. However, major limitations include its poor sensitivity, requirement for rapid sample processing, and inability to differentiate hookworm species nor detect Strongyloides spp. infections. We assessed the prevalence and intensity of STH species in Solomon Islands by conducting a province-wide survey using quantitative PCR (qPCR) for diagnosis, which can provide much better characterisation of STH burden than microscopy. Methodology/Principal findings We conducted a cross-sectional survey in 18 villages in Western Province to detect infections with six STH species and quantify intensity with three. We used linear mixed model regression to identify potential water, sanitation, and hygiene (WASH) and environmental risk factors for infection. We collected stool specimens from 830 village residents. Overall STH prevalence was 63.3% (range 27.5 to 91.5% across villages), led by Necator americanus (54.5% [range 17.5–89.4%]), followed by Ancylostoma ceylanicum (15.5% [range 2.8–45.8%]), Trichuris trichiura (9.1% [range 0–79.2%]), and Strongyloides spp. (3.2% [range 0–29.2%]). Most infections were of light intensity for N. americanus (85.7%) and T. trichiura (90.7%). Owning a household latrine was associated with a lower risk of N. americanus infection (AOR 0.41, 95% CI 0.24–0.68) while greater precipitation was linked to more common T. trichiura infection (AOR 1.14, 95% CI 1.04–1.25). Conclusion/Significance In this first large-scale population survey of STH in the Pacific using qPCR, we found evidence that ivermectin should be incorporated into STH control programmes because of the presence of T. trichiura and Strongyloides spp., both of which are poorly responsive to albendazole. Furthermore, One Health strategies are needed for improved A. ceylanicum and Strongyloides spp. control, WASH access and use should be improved to complement deworming programmes, and control efforts should ideally be expanded to entire communities. Trial registration ClinicalTrials.gov Australian and New Zealand Clinical Trials Registry ACTRN12618001086257.

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Section: Discussionmentioning

confidence: 99%

Using quantitative PCR to identify opportunities to strengthen soil-transmitted helminth control in Solomon Islands: A cross-sectional epidemiological survey

Clarke

Hii

et al. 2022

PLoS Negl Trop Dis

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“…is known to be endemic in Southeast Asia, Sub-Saharan Africa, Latin America, and parts of Southeast United States (Puthiyakunnon et al 2014). Phylogenetic tree research conducted by Bradbury et al (2021) showed that Strongyloides fuelleborni is able to infect human, african or asian ape, macaque, silvery langur and proboscis monkey. This research further prove Strongyloides sp.…”

Section: The Sth Infection Order Dominance In Javan Gibbon and Javan ...mentioning

confidence: 99%

Identification and prevalence of soil-transmitted helminth eggs in Javan gibbon (Hylobates moloch) and Javan langur (Trachypithecus auratus) at Petungkriyono Forest, Central Java, Indonesia

et al. 2022

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Abstract. Kharismawan MYK, Maula I, Astuti P, Setyawan A. 2022. Identification and prevalence of soil-transmitted helminth eggs in Javan gibbon (Hylobates moloch) and Javan langur (Trachypithecus auratus) at Petungkriyono Forest, Central Java, Indonesia. Biodiversitas 23: 4501-4509. Javan gibbon (Hylobates moloch) and Javan langur (Trachypithecus auratus) are arboreal primates found at Petungkriyono Forest, Central Java, Indonesia. An increase in ecotourism activity in Petungkriyono Forest affected wildlife behavior and forest landscape along the road. Canopy connections separated by the main road is essential for both of these primates They were spotted foraging food and moved across the separated habitat using available canopy. Based on their activity and habitat along the main road, this research is conducted to identify and count the prevalance of soil-transmitted helminth eggs in their gastrointestinal through their feces. A total of 72 Javan gibbon and 46 Javan langur fecal samples were collected under 10% formalin preservation. Fecal samples were examined using the mini-FLOTAC method for Javan gibbon and the flotation method for Javan langur. The results indicate three orders, including Enoplida, Strongylida, and Rhabditida, were found and the prevalence of helminthiasis is 31.94% for Javan gibbon and 47.82% for Javan langur. Eventhough both of these primates helminthiasis prevalance is considered to be low, higher prevalance in Javan langur was caused by its behavior to be less arboreal than Javan gibbon.

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“…cebus , and several unidentified Strongyloides spp. are known to infect primates; however, the species greatly differ in their host spectrum (Bradbury et al, 2021). While S .…”

Section: Introductionmentioning

confidence: 99%

“…stercoralis has a broad host specificity and has been detected in a range of hosts including humans, carnivores, and (mainly captive) primates, S . fuelleborni is restricted to primates (mostly free‐ranging) with occasional spillover to humans (Bradbury et al, 2021). Studies using genotyping concluded that S .…”

Section: Introductionmentioning

confidence: 99%

Identification of potentially zoonotic parasites in captive orangutans and semi‐captive mandrills: Phylogeny and morphological comparison

Nosková

Modrý

Baláž

et al. 2023

American J Primatol

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Cysts and trophozoites of vestibuliferid ciliates and larvae of Strongyloides were found in fecal samples from captive orangutans Pongo pygmaeus and P. abelii from Czech and Slovak zoological gardens. As comparative material, ciliates from semi-captive mandrills Mandrillus sphinx from Gabon were included in the study.Phylogenetic analysis of the detected vestibuliferid ciliates using ITS1-5.8s-rRNA-ITS2 and partial 18S ribosomal deoxyribonucleic acid (rDNA) revealed that the ciliates from orangutans are conspecific with Balantioides coli lineage A, while the ciliates from mandrills clustered with Buxtonella-like ciliates from other primates.

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Strongyloides genotyping: a review of methods and application in public health and population genetics

Cited by 23 publications

References 65 publications

Using quantitative PCR to identify opportunities to strengthen soil-transmitted helminth control in Solomon Islands: A cross-sectional epidemiological survey

Using quantitative PCR to identify opportunities to strengthen soil-transmitted helminth control in Solomon Islands: A cross-sectional epidemiological survey

Identification and prevalence of soil-transmitted helminth eggs in Javan gibbon (Hylobates moloch) and Javan langur (Trachypithecus auratus) at Petungkriyono Forest, Central Java, Indonesia

Identification of potentially zoonotic parasites in captive orangutans and semi‐captive mandrills: Phylogeny and morphological comparison

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