“…The bottles were transferred to a 25 °C shaker oven, and were shaken at a speed of 175 rpm. Samples were removed at the following times: 1,4,8,14,22,28,37, and 43 days. The samples were removed and filtered through a 0.1-μm filter, and an aliquot of the filtrate was acidified with an equal volume of 5 M HNO 3 .…”