Strontium enhances proliferation and osteogenic behavior of periodontal ligament cells in vitro

Abstract

Periodontal ligament cells responded to Sr4 with increased cellular proliferation and osteogenic behavior in vitro.

Strontium enhances proliferation and osteogenic behavior of bone marrow stromal cells of mesenchymal and ectomesenchymal origins in vitro

“…In opposition to that, the Sr concentration (Sr4) used in the present study was selected to be comparable to that reported as possible in an actual in vivo system regarding local delivery of Sr (Andersen et al, ) from implant surfaces, and as mentioned, a previous study from our group showed similar effects regarding bone formation for another cell population (PDL cells; Bizelli‐Silveira et al, ). In that study, ratifying the osteogenic action of Sr, undifferentiated cells from the periodontal ligament showed higher OCN and OPN gene expression, together with more evident formation of mineralized nodules when the medium was supplemented with high (360 mg/L) Sr concentrations (Bizelli‐Silveira et al, ). In the present study, the tested concentrations ranged from the physiological level of Sr in humans to thousands of time this value, but within the limits of what could be actually released from an implant surface, and accumulate in the bone‐to‐implant interface (Andersen et al, ).…”

“…These Sr concentrations, though, were selected considering the treatment of bone pathologies such as osteoporosis (suggested dose of 2 mg of SrRan per day; Meunier et al, 2009;Reginster et al, 2005). In opposition to that, the Sr concentration (Sr4) used in the present study was selected to be comparable to that reported as possible in an actual in vivo system regarding local delivery of Sr (Andersen et al, 2013) from implant surfaces, and as mentioned, a previous study from our group showed similar effects regarding bone formation for another cell population (PDL cells; Bizelli-Silveira et al, 2018). In that study, ratifying the F I G U R E 7 Representative images of the alizarin red staining from the fibula for Ca deposits (bone nodules) for the Sr1-4 concentrations at the two evaluation periods (n = 3 samples per group/period).…”

“…Besides the assessment of osteogenic marker levels based on gene expression, the determination of in vitro mineralization is also crucial in evaluating terminal osteogenic differentiation of BMSCs. Because Sr is known to exert dose‐dependent increase in mineralization (Bizelli‐Silveira et al, ; Brennan et al, ; Marie, ), it is not surprising to observe robust mineralization of extracellular matrix in the highest concentrations of Sr, as detected by ARS.…”

“…The pathway determining the anabolic effect of Sr on bone remains poorly understood. In vitro studies suggest that Sr can promote osteoblast proliferation and osteoblastic differentiation in populations rich in precursor cells, such as BMSCs (Bianco et al, 2001;Choudhary, Halbout, Alander, Raisz, & Pilbeam, 2007;Friedenstein, Latzinik, Grosheva, & Gorskaya, 1982;Peng et al, 2009), human adipose-derived stem cells (Aimaiti et al, 2017), and human periodontal ligament cells (PDLCs; Bizelli-Silveira et al, 2018).…”

“…Rats BMSC osteoblastic differentiation was enhanced when the culture medium was supplemented with 1 mM (8.7 mg/L) of strontium ranelate (SrRan), compared with 0.1 mM (87 mg/L; Marie, 2007). A recent study using PDLCs showed higher OCN and OPN gene expression, together with more evident formation of mineralized nodules, when the medium was supplemented with 360 mg/L of Sr, compared with lower Sr concentrations (Bizelli-Silveira et al, 2018).…”