Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response

Capuccini, Chiara; Torricelli, Paola; Sima, Félix; Boanini, Elisa; Ristoscu, Carmen; Bracci, Barbara; Socol, G.; Fini, Milena; Mihăilescu, I. N.; Bigi, Adriana

doi:10.1016/j.actbio.2008.05.005

Cited by 337 publications

(265 citation statements)

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“…However, the biological apatites contain several species as traces such as F − , Cl − , , Na + , Sr 2+ , Mg 2+ , etc. Therefore, the incorporation of one or several of these species into the hydroxyapatite structure should improve its biocompatibility and bioactivity [2,3], and affects its physical and chemical properties such as crystallinity, thermal stability, solubility and osteoconductivity [4][5][6]. Among these latter species, Fluorine is known to delay the caries' processes [7], improve the bonds between the bones and the implant [8][9][10], and strengthen the bone structure 2 3 CO  [11].…”

Section: Introductionmentioning

confidence: 99%

Sintering and Mechanical Properties of Magnesium and Fluorine Co-Substituted Hydroxyapatites

Nsar¹,

Hassine²,

Bouzouita³

2013

JBNB

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Biological apatites contain several elements as traces. In this work, magnesium and fluorine co-substituted hydroxyapatites with the general formula Ca 9 Mg(PO 4 ) 6 (OH) 2-y F y , where y = 0, 0.5, 1, 1.5 and 2 were synthesized by the hydrothermal method. After calcination at 500˚C, the samples were pressureless sintered between 950˚C and 1250˚C. The substitution of F − for OH − had a strong influence on the densification behavior and mechanical properties of the materials. Below 1200˚C, the density steeply decreased for y = 0.5 sample. XRD analysis revealed that compared to hydroxylfluorapatite containing no magnesium, the substituted hydroxyfluorapatites decomposed, and the nature of the decomposition products is tightly dependent on the fluorine content. The hardness, elastic modulus and fracture toughness of these materials were investigated by Vickers's hardness testing. The highest values were 622 ± 4 GPa, 181 ± 1 GPa and 1.85 ± 0.06 MPa·m 1/2 , respectively.

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Section: Introductionmentioning

confidence: 99%

Sintering and Mechanical Properties of Magnesium and Fluorine Co-Substituted Hydroxyapatites

Nsar¹,

Hassine²,

Bouzouita³

2013

JBNB

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“…16 To improve implant osseointegration, osteogenic factors can be used for implant enrichment. Strontium is a low-cost ion and is easier to handle compared to osteoinductive proteins, such as BMP-2, being therefore an ideal candidate to enrich off-the-shelf materials for bone tissue engineering, as proposed for hydroxyapatite 17,18 and, more recently, for titanium. 19,20 Indeed, strontium ranelate is a drug used for the treatment of osteoporosis, thanks to its ability to increase bone neoformation and to reduce bone resorption.…”

Section: Introductionmentioning

confidence: 99%

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Lopa¹,

Mercuri

Colombini³

et al. 2013

J Biomedical Materials Res

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Insufficient implant stability is an important determinant in the failure of cementless prostheses. To improve osseointegration, we aim at generating a bioactive implant combining a macroporous titanium (TT) with a biocompatible hydrogel to encapsulate osteo-inductive factors and osteoprogenitor cells. Amidation and cross-linking degree of an amidated carboxymethylcellulose hydrogel (CMCA) were characterized by FT-IR spectrometry and mechanical testing. Bone marrow mesenchymal stem cells (BMSCs) from osteoarthritic patients were cultured on CMCA hydrogels, TT, and TT loaded with CMCA (TT þ CMCA) with an optimized concentration of SrCl 2 to evaluate cell viability and osteo-differentiation. Amidation and cross-linking degree were homogeneous among independent CMCA batches. SrCl 2 at 5 lg/mL significantly improved BMSCs osteo-differentiation increasing calcified matrix (P < 0.01), type I collagen expression (P < 0.05) and alkaline phosphatase activity. TT þ CMCA samples better retained cells into the TT mesh, significantly improving cell seeding efficiency with respect to TT (P < 0.05). BMSCs on TT þ CMCA underwent a more efficient osteo-differentiation with higher alkaline phosphatase (P < 0.05) and calcium levels compared to cells on TT. Based on these in vitro results, we envision the association of TT with strontium-enriched CMCA and BMSCs as a promising strategy to generate bioactive implants promoting bone neoformation at the implant site.

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“…As was previously reported, Sr ions have been shown to enhance bone collagen synthesis and decrease bone resorption by inhibiting osteoclast resorbing activity [13][14][15][16][17][18][19][20] . Therefore, it is expected that a local release of Sr ions from implant surfaces would directly improve implant osseointegration.…”

Section: Introductionmentioning

confidence: 62%

Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation

et al. 2016

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INTRODUCTIONTitanium (Ti) and its alloys have been used in dentistry as implant materials because of their good mechanical properties, high corrosion resistance, and low cytotoxicity. However, their poor bone fusion properties limit their clinical applications 1) . Various techniques have been explored to modify the surfaces of Ti and its alloys to improve their osseointegration, including plasma spraying, sol-gel method, and electrochemical deposition [2][3][4][5][6][7][8][9][10][11][12] . Among these modification methods, micro-arc oxidation (MAO) has attracted special attention since it is a relatively convenient and effective technique for depositing oxide coatings on the surfaces of nonferrous metals [9][10][11][12] . More importantly, by using this technique, various desired elements can be introduced into the coated layers with porous structures 11) , such as calcium (Ca), phosphorus (P), magnesium (Mg) and strontium (Sr), which are trace elements in the human body playing a significant role in bone formation.Sr is considered a bioactive element that improves implant osseointegration. As was previously reported, Sr ions have been shown to enhance bone collagen synthesis and decrease bone resorption by inhibiting osteoclast resorbing activity [13][14][15][16][17][18][19][20] . Therefore, it is expected that a local release of Sr ions from implant surfaces would directly improve implant osseointegration.Since our previous studies have shown that MAO is a promising method to produce porous and firmly adherent bone formation concerned elements contained oxide deposition layers on metal substrates 11) , it is easy to optimize the Sr amounts introduced into the oxide deposition layers by altering MAO electrolyte compositions. Moreover, except for the first several days, a long-term stable release of metallic ions from the oxide deposition layers can be achieved by using the microporous structures formed during the MAO process 8) . In this study, Ti surfaces were modified by MAO using electrolytes with different Sr concentrations to promote calcification of preosteoblasts. Morphologies and elemental compositions of the surfaces as well as cross-sections of the Ti specimens obtained with and without MAO were characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). A mouse preosteoblast MC3T3-E1 was utilized to study the calcification effect of Sr contents in the MAO-deposited layers on the Ti substrates in vitro. Osteogenic differentiation and calcified depositions of the MC3T3-E1 cells cultured on all specimens were evaluated using alkaline phosphatase (ALP) activity assay and alizarin red s staining, respectively. MATERIALS AND METHODS Ti substrate preparationCommercially pure Ti discs (grade 2, 8 mmφ×2.0 mm in thickness, Rare Metallic, Tokyo, Japan) were used in this study. Before the MAO treatment, Ti discs were mechanically polished with up to #800-grid SiC abrasive paper and then ultrasonically rinsed in acetone and ethanol. The obtained specimens (not subjected to...

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Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response

Cited by 337 publications

References 28 publications

Sintering and Mechanical Properties of Magnesium and Fluorine Co-Substituted Hydroxyapatites

Sintering and Mechanical Properties of Magnesium and Fluorine Co-Substituted Hydroxyapatites

Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium

Effect of strontium ions on calcification of preosteoblasts cultured on porous calcium- and phosphate-containing titanium oxide layers formed by micro-arc oxidation

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