Strontium90 for determination of time since death

Neis, Peter; Hille, R.; Paschke, Mark W.; Pilwat, G.; Schnabel, Alexander; Niess, Constanze; Bratzke, H.

doi:10.1016/s0379-0738(98)00175-3

Cited by 29 publications

(12 citation statements)

References 9 publications

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“…Several other methods for death time estimation using chemical methods could be addressed: enzyme activity in various tissues and organs [15,18]; morphological changes [6,44,59,63]; further analytes in vitreous humor and CSF [10,24] or other body tissues [42]; protein degradation [47].…”

Section: Discussionmentioning

confidence: 99%

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

Madea

2005

Forensic Science International

181

101

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Section: Discussionmentioning

confidence: 99%

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

Madea

2005

Forensic Science International

181

101

View full text Add to dashboard Cite

“…New lines of research such as protein, RNA, and DNA degradation are starting to be unravelled that may provide more precise methods for PMI determination [3][4][5]. Other biochemical markers include analysis of postmortem levels of urea, creatinine, glucose, iron, potassium, calcium, insulin in pancreatic β-cells, strontium90, myo-albumin, myofibril proteins, and several enzymes [6][7][8][9][10]. A previous study by Kang et al.…”

Section: Introductionmentioning

confidence: 99%

Determining time of death: temperature-dependent postmortem changes in calcineurin A, MARCKS, CaMKII, and protein phosphatase 2A in mouse

Poloz

O’Day

2009

Int J Legal Med

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While the determination of postmortem interval (PMI) is a crucial and fundamental step in any death investigation, the development of appropriate biochemical methods for PMI estimation is still in its infancy. This study focused on the temperature-dependent postmortem degradation of calcineurin A (CnA), calmodulin-dependent kinase II (CaMKII), myristoylated alanine-rich C-kinase substrate (MARCKs), and protein phosphatase 2A (PP2A) in mice. The results show that MARCKS, CaMKII, and the use of lung tissue do not appear to warrant further study for the determination of PMI in humans. In skeletal muscle, CnA underwent a rapid temperature-dependent cleavage (60 --> 57 kDa) over the first 48 h of postmortem interval. At 21 degrees C, this transformation was completed within 24 h. In contrast, PP2A increased within the first 24 h after which it degraded at 21 degrees C but remained stable for up to 96 h at 5 degrees C and 10 degrees C. The 60 --> 57 kDa postmortem conversion of CnA was inhibited by addition of protease inhibitors and MDL-28170 indicating a calpain pathway mediates this breakdown. Proteasome inhibition (MG-132) and calmodulin antagonism (calmidazolium) also inhibited this conversion suggesting that other protein degradation pathways also are in play. In contrast, all of the protease inhibitors and calmidazolium but not ethylene glycol tetraacetic acid led to increased levels of PP2A. The data are discussed in terms of developing a useable field-based biochemical assay for postmortem interval determination in humans and understanding the protein degradation pathways that are initiated upon death.

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“…At present, only isotope dating methods seem to provide valid information for the assessment of the postmortem interval (PMI) of skeletal human remains. These techniques are, however, expensive, and their reliability has so far only been tested on small samples [9,12,[16][17][18][19][20][21]. Recently, Verhoff and Kreutz systematically reviewed published, commonly used methods in their literature survey and, on the basis of their results, developed their own recommendations for a practical approach [3].…”

Section: Introductionmentioning

confidence: 99%

Dating skeletal remains with luminol-chemiluminescence. Validity, intra- and interobserver error

Ramsthaler

Kreutz²,

Zipp³

et al. 2009

Forensic Science International

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Strontium90 for determination of time since death

Cited by 29 publications

References 9 publications

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

Determining time of death: temperature-dependent postmortem changes in calcineurin A, MARCKS, CaMKII, and protein phosphatase 2A in mouse

Dating skeletal remains with luminol-chemiluminescence. Validity, intra- and interobserver error

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