Structural alterations of erythrocyte membrane components induced by exhaustive exercise

Brzeszczyńska, Joanna; Pieniążek, Anna; Gwoździński, Łukasz; Gwoździński, Krzysztof; Jegier, A.

doi:10.1139/h08-125

Cited by 23 publications

(36 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar observations, rigidity in erythrocyte membranes, were found in four sedentary dogs after submaximal exercise in a study attempting to elucidate the role of vitamin E supplementation in preventing the effects of submaximal exercise-induced oxidative damage over a long period of time (Motta et al 2009). Finally, Brzeszczynska et al (2008) detected erythrocyte rigidity 1 h after an exhaustive incremental cycling test performed in 11 healthy untrained males. These authors employed three spin labels, 5-, 12-or 16-doxylstearic acids to provide information of changes in the microenvironment in the nearest region of different depths of membrane bilayers.…”

Section: Discussionmentioning

confidence: 99%

“…These authors employed three spin labels, 5-, 12-or 16-doxylstearic acids to provide information of changes in the microenvironment in the nearest region of different depths of membrane bilayers. Since an increase in the order was detected only for the 5-doxylstearic acid spin label, they concluded that exercise-induced decreases in erythrocyte membrane fluidity were located in the polar region of the membranes (Brzeszczynska et al 2008). To measure fluidity changes in this specific region of the membrane, we chose TMA-DPH because the polar region of this probe is anchored at the lipid-water interface, while the hydrocarbon moiety enters the lipid part of the membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, cell membranes isolated from RBC may reflect, at least partially, processes ongoing at various sites in the organism such as skeletal muscles. For this reason and because of its ready availability, membranes isolated from RBC have been used to perform experimental studies on the membrane function in animals (Motta et al 2009;Portier et al 2006) and in humans (Brzeszczynska et al 2008). None of these studies have compared different types of exercise and correlated the membrane function with the plasma oxidative damage.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

et al. 2010

View full text Add to dashboard Cite

Optimal levels of membrane fluidity are essential for numerous cell functions including cell growth, solute transport and signal transduction. Since exercise enhances free radical production, our aim was to evaluate in healthy male subjects the effects of an acute bout of maximal and submaximal exercise on the erythrocyte membrane fluidity and its possible relation to the oxidative damage overproduction due to exercise. Subjects (n = 34) performed three cycloergometric tests: a continuous progressive exercise, a strenuous exercise until exhaustion and an acute bout of exercise at an intensity corresponding to 70% of maximal work capacity for 30 min. Venous blood samples were collected before and immediately after these exercises. Erythrocyte membrane fluidity was assessed by fluorescence spectroscopy. Plasma malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations and carbonyl content of plasmatic proteins were used as an index of lipid and protein oxidation, respectively. Exercise produced a dramatic drop in the erythrocyte membrane fluidity as compared to resting time, but this was not accompanied by significant changes in the plasmatic MDA and 4-HDA concentrations. The highest erythrocyte membrane rigidity was detected immediately after strenuous exercise until exhaustion was performed. Protein carbonyl levels were higher after exhaustive exercises than at rest. Continuous progressive and strenuous exercises until exhaustion, but not submaximal workload, resulted in a significant enhanced accumulation of carbonylated proteins in the plasma. These findings are consistent with the idea that exercise exaggerates oxidative damage, which may contribute, at least partially, to explain the rigidity in the membrane of the erythrocytes due to acute exercise.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

et al. 2010

View full text Add to dashboard Cite

show abstract

“…We think that RBC destruction is one important reason leading to anemia [15,16] . The cause of RBC destruction is discussed below.…”

Section: Discussionmentioning

confidence: 99%

A novel acute anemia model for pharmacological research in mice by compelled acute exercise

et al. 2009

View full text Add to dashboard Cite

Aim: To generate a novel acute anemia model by compelled exercise. Methods: Young adult male mice were compelled to perform an acute exercise by electric stimulation for 30 min. Blood was taken from their tails to be used for detection immediately after exercise. Results: It was found that the red blood cell (RBC) count, hemoglobin concentration and hematocrit of exhausted mice were lower than that of sedentary mice without electric stimulation. The model is in line with the diagnostic criteria. As the mechanisms of the acute anemia model, it was found that the osmotic fragility of the RBC in model mice increased compared to that of normal mice, and that the deformation index, orientation index, small deformation index, deformability and orientation of RBCs in the model mice were reduced. The glutathione (GSH) levels in blood plasma were decreased. The liver, spleen, kidney had no detectable changes. Conclusion: To the best of our knowledge, these effects represent the success of a novel anemia model by compelled acute exercise. The oxidative damages on RBC and hemorheological changes are in part the causes of acute anemia in this model.

show abstract

“…Он наступает вcледствие нарушения баланса между образованием и удалeнием активированных кислородных интермедиатов -таких, как пероксид водорода (H 2 O 2 ), супероксидный анион (O 2 -) и другие радикалы. Результатом окислительного повреждения мембранных белков и липидов является снижение текучести клеточной мембраны эритроцита [4]. Выявлено антиокислительное действие ингибиторов 3-гидрокси-3-метилглутарил-CoA-редуктазы (КФ 1.1.1.88) (статинов), не зависящее от их гипохолестеринемического влияния [5].…”

unclassified

Antioxidant enzymes in erythrocytes from hypertension patients receiving lisinopril monotherapy or combined lisinopril plus simvastatin therapy

Kosenko

Av²,

et al. 2011

BIOMED KHIM

View full text Add to dashboard Cite

Statins and angiotensin-converting enzyme (ACE) inhibitors have beneficial impact on the serum cholesterol and blood pressure. It is supposed that statins and ACE inhibitors may modify the antioxidative status of erythrocytes. The study objective was to compare the effects of two treatments, lisinopril alone vs lisinopril plus simvastatin, on erythrocyte antioxidant enzyme activities. The study involved 32 patients with arterial hypertension, the initial serum total cholesterol, LDL-cholesterol and triglycerides within the normal range. Patients of two groups, each of 16 subjects, were treated with lisinopril (10 mg/day) or with lisinopril (10 mg/day) plus simvastatin (20 mg/day). Before and after 3 and 6 months of follow-up therapy, activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GLR) in purified erythrocytes were determined. In all patients, significantly higher catalase activity (by 79.3-106.5%, p<0.0001) and significantly lower GPx activity (by 20.7-30.6%, p<0.001) were observed after therapy as compared to the baselines. Just the same results were obtained in both groups (lisinopril and lisinopril + simvastatin), after both periods (3 and 6 month) of treatments. SOD activity was increased only in the lisinopril group and only after 6 months (p=0.0345). No changes of GLR reductase activity were seen under all conditions indicated. Thus, the lisinopril monotherapy and combined lisinopril plus simvastatin therapy exhibit specific, pronounced and equipotent effects on antioxidant enzymes in human erythrocytes. Administration of lisinopril or lisinopril plus simvastatin may protect erythrocytes and other tissues from oxidative damage.

show abstract

Structural alterations of erythrocyte membrane components induced by exhaustive exercise

Cited by 23 publications

References 37 publications

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

A novel acute anemia model for pharmacological research in mice by compelled acute exercise

Antioxidant enzymes in erythrocytes from hypertension patients receiving lisinopril monotherapy or combined lisinopril plus simvastatin therapy

Contact Info

Product

Resources

About