Structural alterations of the erythrocyte membrane proteins in diabetic retinopathy

Petropoulos, Ioannis K.; Margetis, Panagiotis I.; Antonelou, Marianna H.; Koliopoulos, J; Gartaganis, Sotirios P.; Margaritis, Lukas H.; Papassideri, Issidora S.

doi:10.1007/s00417-006-0500-6

Cited by 18 publications

(12 citation statements)

References 50 publications

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“…[1] Diabetes mellitus induces changes in rheological properties, i.e., specific changes in mechanical properties, e.g., increase in erythrocyte micro-viscosity, aggregation and adherisivness, which causes the changes in lipid composition, dysfunctioning of membrane structure and composition. [2,3] Erythrocyte membrane being continuously exposed to free radicals and the high-oxidative stress leads to damage in structural components. [4] Free radicals are not only consequence of diabetes mellitus but can also cause diabetes by damaging beta cells of the pancreas.…”

Section: Introductionmentioning

confidence: 99%

Biochemical changes in erythrocyte membrane in type 2 diabetes mellitus

Kumar

2012

Indian J Med Sci

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Section: Introductionmentioning

confidence: 99%

Biochemical changes in erythrocyte membrane in type 2 diabetes mellitus

Kumar

2012

Indian J Med Sci

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“…Ogata et al reported increased levels of platelet- and monocyte-derived microparticles (MPs) in the plasma of DR patients [4, 5]; however, whether phosphatidylserine-positive (PS + ) MPs contribute to PCA in DR and the precise role of MPs in prothrombinase and fibrin formation remain poorly investigated. Moreover, the contribution of peripheral blood cells (PBCs) to the procoagulant state in DR is also poorly characterized, although red blood cells (RBCs) and platelets appear to aggregate in blood vessels [6, 7]. Inflammatory responses are closely associated with the pathogenesis of DR, and leukostasis has been found in the retina of diabetic animals [8-10].…”

Section: Introductionmentioning

confidence: 99%

Procoagulant Activity of Blood and Endothelial Cells via Phosphatidylserine Exposure and Microparticle Delivery in Patients with Diabetic Retinopathy

Chen

Dong

et al. 2018

Cell Physiol Biochem

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Background/Aims: The mechanisms for thrombosis in diabetic retinopathy (DR) are complex and need to be further elucidated. The purpose of this study was to test phosphatidylserine (PS) exposure on microparticles (MPs) and MP-origin cells from the circulation and to analyze cell-/MP-associated procoagulant activity (PCA) in DR patients. Methods: PS-positive MPs and cells from healthy controls (n = 20) and diabetic patients (n = 60) were analyzed by flow cytometry and confocal microscopy. Clotting time and purified coagulation complex assays were used to measure PCA. Results: PS exposure on platelets and monocytes was higher in proliferative DR (PDR) patients than in non-PDR patients or controls. The highest levels of MPs (derived from platelets [30%], erythrocytes [13%], leukocytes [28%], and endothelial cells [10%]) were found in patients with PDR. In addition, PS exposure on blood cells and shed MPs in DR patients led to significantly increased FXa and FIIa generation, fibrin formation, and markedly shortened coagulation time. Moreover, lactadherin reduced 70% of PCA by blocking PS, while an anti-tissue factor antibody had a smaller effect. Conclusion: Our results confirmed that PCA in DR patients may be partly ascribed to PS exposure and MP release from blood and endothelial cells. Lactadherin may act as an efficient anticoagulant factor in this process.

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“…Although smaller vessel diameters could explain the decreases, no change in capillary diameter could be documented in the hyperglycemic rats. Increased resistance of blood flow through the capillaries could be the result of a decrease in erythrocyte deformability and a concominant increase in blood viscosity caused by the hyperglycemia (Fornal et al, 2006; Petropoulos et al, 2007; Tsukada et al, 2001). Increased resistance within the choriocapillaris could also be caused by an increase in adhesion of leukocytes to the endothelium, since there is an increase in the expression of intercellular adhesion molecule-1and P-selectin on the endothelium in human diabetic choroid (McLeod et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…In arterioles, uneven RBC distribution is mainly a result of plasma skimming, although any factor that results in asymmetry of the RBC profile in the parent vessel increases the role of red cell screening at the bifurcation and enhances the plasma skimming effect (Pries et al, 1989). Therefore, any diabetes-induced decrease in RBC deformability (Fornal et al, 2006; Petropoulos et al, 2007; Tsukada et al, 2001) or alterations in the interaction between RBCs and the endothelium (Nieuwdorp et al, 2006; Raz et al, 1988) could lead to an increase in plasma skimming at arteriolar bifurcations.…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte flow in choriocapillaris of normal and diabetic rats

Braun

Wienczewski

Abbas

2009

Microvascular Research

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The choriocapillaris is a unique capillary bed that provides nutrients to the retinal photoreceptors. It changes anatomically in diabetes, but the impact of these changes on blood flow is unknown. In this study hemodynamic parameters in individual choriocapillaris vessels were compared in normal and diabetic rats. Three groups were studied: normal buffer-injected control rats, streptozotocin (STZ)-injected mildly hyperglycemic (STZ-MH) rats, and STZ-injected diabetic (STZ-D) rats. 7-8 weeks after STZ injection, the rats were anesthetized, and epifluorescent, intravital microscopy was used to record the flow of fluorescent red blood cells (RBC) in the choriocapillaris. Diameter, RBC flux, and RBC velocity were measured in 153 capillary pathways in five control rats, 98 pathways in four STZ-MH rats, and 153 pathways in seven STZ-D rats. There was no difference in capillary diameter among the groups. RBC flux and velocity were lower in the STZ-injected rats compared to the controls (p≤0.023), which is similar to changes found in other capillary beds. RBC velocity and flux were significantly correlated in all three groups, but the correlations in the STZ-injected rats were much stronger than in the controls. This indicates a more heterogeneous distribution of RBCs at upstream arteriolar branchpoints in hyperglycemic rats, which could lead to a decrease in choriocapillaris hematocrit. These changes in the hyperglycemic choriocapillaris could contribute to impaired oxygen delivery to the photoreceptors in diabetic retina.

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Structural alterations of the erythrocyte membrane proteins in diabetic retinopathy

Cited by 18 publications

References 50 publications

Biochemical changes in erythrocyte membrane in type 2 diabetes mellitus

Biochemical changes in erythrocyte membrane in type 2 diabetes mellitus

Procoagulant Activity of Blood and Endothelial Cells via Phosphatidylserine Exposure and Microparticle Delivery in Patients with Diabetic Retinopathy

Erythrocyte flow in choriocapillaris of normal and diabetic rats

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