Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry

Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta

“…Negative-ion tandem ESIMS of monosulfated Fuc ion at m/z 243.02 (not shown) matched with that already observed for other Laminariales (Anastyuk et al, 2012a(Anastyuk et al, , 2010. Signals from C4 (m/z 183.00) and C2 (m/z 138.97) sulfation of ␣-L-Fucp residues were equal.…”

“…Probably, Gal residues were terminal to the chains, built up of fucose residues, like it was observed for S. latissima (Bilan et al, 2010). Negative-ion tandem MALDI-TOF spectra of singly-sulfated fucobiose (m/z 389.1) and fucotriose (m/z 535.1) as well as doubly-sulfated fucobiose (m/z 491.0) were typical for (1→3)-linked fucooligosaccharides observed earlier for fucans from S. cichorioides, S. gurjanovae and Costaria costata (Anastyuk et al, 2012a(Anastyuk et al, , 2010Shevchenko et al, 2015), since no intensive signals of cross-ring cleavages of A-or X-type were observed (see Supplementary materials).…”

“…The fucan core of galactofucan AaF3 from A. angusta thus contained the main chain identical with already investigated fucoidans from S. cichorioides, S. gurjanovae, S. latissima and others (Anastyuk et al, 2010;Bilan et al, 2010;Cumashi et al, 2007;Zvyagintseva et al, 2003).…”

“…Shevchenko et al, 2015) proven to be reliable and reproducible method for soft depolymerization of highly sulfated fucans/fucoidans to produce mixtures of low-molecular-weight (LMW) oligosaccharides, carrying structural information. Recently, by using this technique on the galactofucan from Saccharina gurjanovae and the fucan from Saccharina cichorioides (Laminariales) with known (Anastyuk et al, 2010;Zvyagintseva et al, 2003) structure, we have obtained highmolecular-weight (HMW) fractions which were built up of only 3-linked 4-sulfated ␣-L-Fucp residues (Shevchenko et al, 2015). The structure of the native main chain then was assumed to be represented by a repeating unit →3)-␣-L-Fucp-(2,4-SO 3 − )-(1→ that was shown to be characteristic to Laminariales (Anastyuk et al, 2010;Bilan et al, 2010;Cumashi et al, 2007;Zvyagintseva et al, 2003).…”

Fucoidan, A Sulfated Polysaccharides from Brown Algae as Therapeutic Target for Cancer

“…Fucoidan extracted from brown seaweed algae Fucus vesiculosus [24], Ascophyllum nodosum [25], Sargassum kjellmanianum [26], Sargassum thunbergii [27], Cladosiphon okamuranus Tokida [28], in which the percentage of L-fucose ranged from 12.6 to 36.0 %, and the percentage of sulfate content from 8 to 25 %. The isolated and determined structural characteristics of the fucoidans from brown algae Saccharina cichorioides, Fucus evanescens, and Undaria pinnatifida [29][30][31] and S. cichorioides consisted of (1 → 3)-α-L-fucose residues, fucoidan from F. evanescens contained alternating (1 → 3)-and (1 → 4)-α-L-fucose, while fucoidan from U. pinnatifida was built up of (1 → 3)-and (1 → 4)-linked α-L-fucose and β-D-galactose residues [32,33]. The structure of fucoidan is depicted in Fig.…”