Structural analysis of doxorubicin-polymer conjugates

Sanyakamdhorn, S.; Bekale, L.; Agudelo, Daniel; Tajmir-Riahi, H.A.

doi:10.1016/j.colsurfb.2015.07.070

Cited by 28 publications

(8 citation statements)

References 41 publications

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“…The TEM analysis of the free FA-PAMAM-G3 and FA-PAMAM-G4 and their DNA and tRNA conjugates in aqueous solution at pH 7.2 are shown in Figures 5 and 6. The TEM images of the free DNA and tRNA show major spherical aggregates, with particle sizes ranging from 3 to 10 nm with a mean diameter of 5 to 6 nm (Figures 5A and 6A), which is in agreement with literature reports [40][41][42][43][44][45]. Marked differences were also observed in the morphology of the FA-PAMAM aggregates.…”

Section: Effect Of Folic Acid-polymer Conjugation On Dna and Trna Morphologysupporting

confidence: 90%

Functionalized Folic Acid with Chitosan and PAMAM Dendrimers for Delivery of DNA and RNA

2021

J Nanotechnol Nanomaterials

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In this review, we explore the potential applications of functionalized folic acid-polymer nanoparticles in nucleic acids delivery. Folic acid-chitosan (carbohydrates) and folic acid-PAMAM (dendrimers) of different polymer sizes were used to conjugate with DNA and tRNA in vitro. Thermodynamic analysis showed that conjugation of DNA and tRNA to nanoparticles occurred via hydrophilic, hydrophobic, H-bonding and van der Waals contacts. Major alterations of DNA and RNA morphology occurred by nanocarrier interaction. These results indicate that functionalized folic acid-nanoparticles can deliver DNA and RNA to target sites.

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Section: Effect Of Folic Acid-polymer Conjugation On Dna and Trna Morphologysupporting

confidence: 90%

Functionalized Folic Acid with Chitosan and PAMAM Dendrimers for Delivery of DNA and RNA

2021

J Nanotechnol Nanomaterials

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“…PAMAM-G4 forms more stable conjugates than PAMAM-G3. Similar structural changes were observed for several drug-PAMAM-conjugates [42,43].…”

Section: Tem Images and The Morphology Of Testosteronepolymer Aggregatessupporting

confidence: 80%

Review on Testosterone Delivery by Natural and Synthetic Nanoparticles

Riahi¹

2017

JNMR

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IntroductionNatural and synthetic polymers are widely used as drug delivery tools in pharmaceutical and nanomedicine biotechnology [1][2][3][4][5]. Among synthetic polymers, dendrimers, a family of cationic polymers, are promising nonviral vectors for gene and drug delivery because of a well-defined molecular shape, controlled chemical structure, high water solubility, large number of chemically versatile surface groups, and unique architecture [6][7][8]. Natural cationic polymers such as chitosan are attractive candidates for therapeutic applications as they generally are nontoxic, being derived from renewable resources [9]. Biodegradable, biocompatible and nontoxic chitosan nanoparticles are of a major interest in drug delivery systems. They form complexes with antibiotics, anticancer drugs and therapeutic proteins [10][11][12]. Chitosan and its derivatives have the desired properties for safe use as pharmaceutical drug delivery tools. This has prompted accelerated research activities worldwide on chitosan nanoparticles as drug delivery tools [13][14][15][16].Testosterone is the main androgenic hormone which controls many physiological processes such as, sexual functions and secondary sex characteristics, muscle protein metabolism, plasma lipid and bone metabolism [17]. Testosterone can also be used as a natural template to construct semi-synthetic analogues such as dimers, testosterone amide derivatives, as well as testosteronecytotoxic hybrid molecules [18][19][20][21][22]. These novel semi-synthetic molecules can be tested for their biological properties, using diverse spectroscopic methods [18][19][20][21][22][23][24][25]. Synthetic polymers are used as potential nanocarriers to deliver steroids in vitro and in vivo [26][27][28]. Furthermore, chitosan and its derivatives were also tested as delivery tools for transporting steroids [29,30].In this review, the binding efficacies of testosterone with PAMAM dendrimers and chitosan nanoparticles were compared, using multiple spectroscopic, TEM images and molecular modeling results. Structural analysis regarding testosterone binding process and the effect of steroid-polymer conjugation on polymer morphology as well as the possibility of testosterone delivery by PAMAM and chitosan nanoparticles are discussed here. Experimental Transmission electron microscopyThe TEM images were taken using a Philips EM 208S microscope operating at 180 kV. The morphology of the testosterone conjugates with PAMAM dendrimers and chitosan nanoparticles in aqueous solution at pH 7.4 were observed, using transmission electron microscopy. One drop (5-10 µL) of the freshly-prepared mixture [polymer solution (60 µM) + steroid solution (60 µM)] in Tris-HCl buffer (24 ± 1°C) was deposited onto a glow-discharged carbon-coated electron microscopy grid. The excess liquid was absorbed by a piece of filter paper, and a drop of 2% uranyl acetate negative stain was added before drying at room temperature [28]. UV spectroscopyThe UV-Vis spectra were recorded on a Perkin-Elmer Lambda AbstractThe lo...

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“…The internal cavities of dendrimers, a characteristic of their structure, are in most cases hydrophobic and allow the interaction with poorly soluble drugs. Sanyakamdhorn et al described a thorough investigation of the intramolecular interaction of DOX and tamoxifen, and their respective analogs, with dendrimers [ 122 , 123 ]. They showed that the formation of hydrogen bonds between the drugs and the -NH groups in the interior of PAMAM, as well as hydrogen bonding and electrostatic interactions with surface amino groups, were responsible for the physical encapsulation of the drugs.…”

Section: Dendrimers As Drug Delivery Carriers In Cancer Therapymentioning

confidence: 99%

Dendrimers as Nanocarriers for Nucleic Acid and Drug Delivery in Cancer Therapy

2017

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Dendrimers are highly branched polymers with easily modifiable surfaces. This makes them promising structures for functionalization and also for conjugation with drugs and DNA/RNA. Their architecture, which can be controlled by different synthesis processes, allows the control of characteristics such as shape, size, charge, and solubility. Dendrimers have the ability to increase the solubility and bioavailability of hydrophobic drugs. The drugs can be entrapped in the intramolecular cavity of the dendrimers or conjugated to their functional groups at their surface. Nucleic acids usually form complexes with the positively charged surface of most cationic dendrimers and this approach has been extensively employed. The presence of functional groups in the dendrimer’s exterior also permits the addition of other moieties that can actively target certain diseases and improve delivery, for instance, with folate and antibodies, now widely used as tumor targeting strategies. Dendrimers have been investigated extensively in the medical field, and cancer treatment is one of the greatest areas where they have been most used. This review will consider the main types of dendrimer currently being explored and how they can be utilized as drug and gene carriers and functionalized to improve the delivery of cancer therapy.

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Structural analysis of doxorubicin-polymer conjugates

Cited by 28 publications

References 41 publications

Functionalized Folic Acid with Chitosan and PAMAM Dendrimers for Delivery of DNA and RNA

Functionalized Folic Acid with Chitosan and PAMAM Dendrimers for Delivery of DNA and RNA

Review on Testosterone Delivery by Natural and Synthetic Nanoparticles

Dendrimers as Nanocarriers for Nucleic Acid and Drug Delivery in Cancer Therapy

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