Structural analysis of linear hydroxyproline-bound O-glycans of Chlamydomonas reinhardtii—conservation of the inner core in Chlamydomonas and land plants

Bollig, Klaus; Lamshöft, Marc; Schweimer, Kristian; Marner, Franz‐Josef; Budzikiewicz, H.; Waffenschmidt, Sabine

doi:10.1016/j.carres.2007.08.008

Cited by 79 publications

(76 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Hyp-b-L-arabinofuranoside linkage has been conserved since Chlamydomonas (Miller et al, 1972;Bollig et al, 2007). Thus, the conservation of the SerHyp 4 glycomodule implies a crucial function, yet the module is uncharged and chemically unreactive under physiological conditions.…”

Section: Why Are Prps Not Glycosylated Like Agps?mentioning

confidence: 99%

Role of the Extensin Superfamily in Primary Cell Wall Architecture

et al. 2011

View full text Add to dashboard Cite

Section: Why Are Prps Not Glycosylated Like Agps?mentioning

confidence: 99%

Role of the Extensin Superfamily in Primary Cell Wall Architecture

et al. 2011

View full text Add to dashboard Cite

“…Studies on glycosylation pathways in C. reinhardtii have been mostly focused on O-glycosylation processing, as the cell wall of this organism consists of a vast framework of O-glycosylated hydroxyproline-rich glycoproteins (2,3). More recently, Bollig et al even demonstrated that Oglycans from C. reinhardtii cell wall glycoproteins contain arabinose and galactose, the latter being in the furanose form (4). In contrast, the N-glycosylation pathway, although a major post-translational modification step in the maturation of secreted proteins in eukaryotes, has received very little attention so far.…”

mentioning

confidence: 99%

Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtii Unravels Novel Complex Structures

Mathieu‐Rivet

Scholz

Arias

et al. 2013

Molecular & Cellular Proteomics

142

View full text Add to dashboard Cite

Chlamydomonas reinhardtii is a green unicellular eukaryotic model organism for studying relevant biological and biotechnological questions. The availability of genomic resources and the growing interest in C. reinhardtii as an emerging cell factory for the industrial production of biopharmaceuticals require an in-depth analysis of protein N-glycosylation in this organism. Accordingly, we used a comprehensive approach including genomic, glycomic, and glycoproteomic techniques to unravel the N-glycosylation pathway of C. reinhardtii. Using mass-spectrometry-based approaches, we found that both endogenous soluble and membrane-bound proteins carry predominantly oligomannosides ranging from Man-2 to Man-5. In addition, minor complex N-linked glycans were identified as being composed of partially 6-Omethylated Man-3 to Man-5 carrying one or two xylose residues. These findings were supported by results from a glycoproteomic approach that led to the identification of 86 glycoproteins. Here, a combination of in-source collision-induced dissodiation (CID) for glycan fragmentation followed by mass tag-triggered CID for peptide sequencing and PNGase F treatment of glycopeptides in the presence of 18 O-labeled water in conjunction with CID mass spectrometric analyses were employed. In conclusion, our data support the notion that the biosynthesis and maturation of N-linked glycans in the endoplasmic reticulum and Golgi apparatus occur via a GnT I-independent pathway yielding novel complex N-linked glycans that maturate differently from their counterparts in land plants. Molecular & Cellular

show abstract

“…It is likely that AGPs in the chlorophyte algae have distinct functions from streptophytes, as no large arabino-3,6-galactan polysaccharides have been reported (Ferris et al, 2001). Short, branched oligosaccharides (Ferris et al, 2001) and linear, Ara-and Gal-containing oligosaccharides (Bollig et al, 2007) have been isolated from C. reinhardtii walls.…”

mentioning

confidence: 99%

Insights into the Evolution of Hydroxyproline-Rich Glycoproteins from 1000 Plant Transcriptomes

et al. 2017

View full text Add to dashboard Cite

The carbohydrate-rich cell walls of land plants and algae have been the focus of much interest given the value of cell wall-based products to our current and future economies. Hydroxyproline-rich glycoproteins (HRGPs), a major group of wall glycoproteins, play important roles in plant growth and development, yet little is known about how they have evolved in parallel with the polysaccharide components of walls. We investigate the origins and evolution of the HRGP superfamily, which is commonly divided into three major multigene families: the arabinogalactan proteins (AGPs), extensins (EXTs), and proline-rich proteins. Using motif and amino acid bias, a newly developed bioinformatics pipeline, we identified HRGPs in sequences from the 1000 Plants transcriptome project (www.onekp.com). Our analyses provide new insights into the evolution of HRGPs across major evolutionary milestones, including the transition to land and the early radiation of angiosperms. Significantly, data mining reveals the origin of glycosylphosphatidylinositol (GPI)-anchored AGPs in green algae and a 3-to 4-fold increase in GPI-AGPs in liverworts and mosses. The first detection of cross-linking (CL)-EXTs is observed in bryophytes, which suggests that CL-EXTs arose though the juxtaposition of preexisting SP n EXT glycomotifs with refined Y-based motifs. We also detected the loss of CL-EXT in a few lineages, including the grass family (Poaceae), that have a cell wall composition distinct from other monocots and eudicots. A key challenge in HRGP research is tracking individual HRGPs throughout evolution. Using the 1000 Plants output, we were able to find putative orthologs of Arabidopsis pollen-specific GPI-AGPs in basal eudicots.Cell walls of plants and algae are widely used for food, textiles, paper, and timber, yet our understanding of their assembly and dynamic remodeling in response to growth, development, and environmental stresses (abiotic and biotic) remains rudimentary (Doblin et al., 2010(Doblin et al., , 2014. There are two contrasting types of walls/extracellular matrices in the green plant lineage, protein-rich walls of some green algae and the cellulose-rich walls of embryophytes (land plants). Recent studies of cell wall evolution suggest that the origins of many wall components occurred in the streptophyte green algae prior to the evolution of embryophytes (Sørensen et al., 2010;Popper et al., 2011;Domozych et al., 2012) with elaboration of a preexisting set of polysaccharides rather than an entirely new polymer framework. Although both the ultrastructure of plant walls and the fine structure of their component polymers vary widely, they all typically constitute a fibrillar network of cellulose microfibrils that is embedded within a gel-like matrix of noncellulosic polysaccharides, pectins, 904

show abstract

Structural analysis of linear hydroxyproline-bound O-glycans of Chlamydomonas reinhardtii—conservation of the inner core in Chlamydomonas and land plants

Cited by 79 publications

References 35 publications

Role of the Extensin Superfamily in Primary Cell Wall Architecture

Role of the Extensin Superfamily in Primary Cell Wall Architecture

Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtii Unravels Novel Complex Structures

Insights into the Evolution of Hydroxyproline-Rich Glycoproteins from 1000 Plant Transcriptomes

Contact Info

Product

Resources

About