Structural analysis of the α-d-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Leeuwen, Sander S. van; Kralj, Slavko; Geel-Schutten, Ineke H. van; Gerwig, Gerrit J.; Dijkhuizen, Lubbert; Kamerling, Johannis P.

doi:10.1016/j.carres.2008.01.042

Cited by 90 publications

(169 citation statements)

References 21 publications

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“…GTF180-ΔN produces a branched dextran built up from different lengths of αð1 → 6Þ-linked glucose residues, interconnected by single αð1 → 3Þ-bridges. No consecutive αð1 → 3Þ glucosidic bonds are present (35). The enzyme must thus be able to transfer the glucosyl residue to either the O6 or the O3 hydroxyl group.…”

Section: Resultsmentioning

confidence: 99%

Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes

Vujicic-Zagar

Pijning

Kralj

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Glucansucrases are large enzymes belonging to glycoside hydrolase family 70, which catalyze the cleavage of sucrose into fructose and glucose, with the concomitant transfer of the glucose residue to a growing α-glucan polymer. Among others, plaque-forming oral bacteria secrete these enzymes to produce α-glucans, which facilitate the adhesion of the bacteria to the tooth enamel. We determined the crystal structure of a fully active, 1,031-residue fragment encompassing the catalytic and C-terminal domains of GTF180 from Lactobacillus reuteri 180, both in the native state, and in complexes with sucrose and maltose. These structures show that the enzyme has an α-amylase-like ðβ∕αÞ 8 -barrel catalytic domain that is circularly permuted compared to the catalytic domains of members of glycoside hydrolase families 13 and 77, which belong to the same GH-H superfamily. In contrast to previous suggestions, the enzyme has only one active site and one nucleophilic residue. Surprisingly, in GTF180 the peptide chain follows a "U"-path, such that four of the five domains are made up from discontiguous N-and C-terminal stretches of the peptide chain. Finally, the structures give insight into the factors that determine the different linkage types in the polymeric product.crystal structure complexes | exopolysaccharide | Lactobacillus reuteri | dental caries

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Section: Resultsmentioning

confidence: 99%

Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes

Vujicic-Zagar

Pijning

Kralj

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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151

262

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“…To our knowledge, our present study is the first to report that a single mutation completely abolished one type of linkage in the synthesis of ␣-glucan by a glucansucrase. The polysaccharide produced by wild-type GTF180-⌬N is built up from different lengths of isomalto-oligosaccharides, interconnected by single (␣133) glycosidic linkages in linear and branched orientations (12% terminal, 24% 3-monosubstituted, 52% 6-monosubstituted, and 12% 3,6-disubstituted ␣-D-glucopyranose residues) (27). The L940W mutant is deficient in the synthesis of (␣133) branch linkages, resulting in the synthesis of linear ␣-glucans.…”

Section: Construction and Expression Of Gtf180-⌬n Leumentioning

confidence: 99%

“…Further studies are required to explore regions that are critical for specificity and to expand the diversity of ␣-glucan products for different applications. In our previous studies, GTF180 of L. reuteri 180 was found to produce dextran with 69% (␣136) linkages and 31% (␣133) linkages (5,27). Truncation of the N-terminal variable domain had no significant effect on the product spectrum of GTF180 (5).…”

mentioning

confidence: 92%

Residue Leu940 Has a Crucial Role in the Linkage and Reaction Specificity of the Glucansucrase GTF180 of the Probiotic Bacterium Lactobacillus reuteri 180

Meng¹,

Dobruchowska²,

Pijning

et al. 2014

Journal of Biological Chemistry

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Background: Glucansucrases of lactic acid bacteria catalyze the synthesis of a variety of ␣-glucans from sucrose. Results: Mutations of Leu 940 in domain B of GTF180 significantly altered its linkage and reaction specificity. Conclusion: Residue Leu 940 of GTF180 is a critical structural determinant for linkage and reaction specificity. Significance: This study provides novel insights into the structural basis of glucansucrases to facilitate the production of tailor-made ␣-glucans.

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“…Nastali biofilm sprečava koroziju čelika pri čemu se pretpostavlja da dolazi do formiranja kompleksnog jedinjenja metalpolisaharid na površini čelika, koji ima zaštitnu ulogu. Van Leeuwen i dr., [49] u svom istraživanju uočili su da D-glukan proizveden od strane Lactobacillus reuteri takođe inhibira koroziju metala. Osim toga, korišćen je i sloj EPS sintetisan od strane Leuconostoc mesenteroides kao prevlaka u cilju zaštite nisko ugljeničnog čelika od korozije [33].…”

Section: Inhibicija Korozije Pomoću Biofilmaunclassified

Microorganisms as potential corrosion inhibitors of metallic materials

Tasić¹,

Antonijević²,

Petrović-Mihajlović³

2016

Rec odr raz

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IZVOD -Proces korozije predstavlja destrukciju kako metalnih tako i nemetalnih materijala usled hemijske ili elektrohemijske interakcije sa okolnom sredinom. Kao rezultat interakcija između površine metala i bakterijskih ćelija, odnosno njihovih metabolita nastaje mikrobiološki indukovana korozija. Međutim, neke vrste mikroorganizama utiču na smanjenje procesa korozije. Shodno tome, analizirana je literatura koja se bavi primenom mikroorganizama kao potencijalnih inhibitora korozije metala. Različite bakterijske vrste ispitivane su kao potencijalni inhibitori korozije. Istaknut je značaj samih mikroorganizama kao i uloga ekstracelularnih polimernih supstanci na koroziono ponašanje metala. Prikazano je da na stepen zaštite pored tipa primenjenog mikroorganizma, utiče i vrsta metala. Takođe, na osnovu prikazanih podataka može se videti da pojedine vrste mikroorganizma mogu uspešno zameniti organske inhibitore koji mogu biti toksični.Ključne reči: mikroorganizmi, korozija, metali, inhibitori, bakterijske vrste ABSTRACT -Corrosion presents the destruction of materials through chemical or electrochemical interactions with their environment. Interactions between the metal surface and bacterial cells or products of their metabolic activities can lead to microbially-influenced corrosion. Also, it is known that certain microorganisms can contribute to corrosion inhibition. In accordance to that, the literature dealing with the application of different microorganisms as a potentialy corrosion inhibitors of metals is investigated. Different bacterial strains as a corrosion

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Structural analysis of the α-d-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

Cited by 90 publications

References 21 publications

Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes

Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes

Residue Leu940 Has a Crucial Role in the Linkage and Reaction Specificity of the Glucansucrase GTF180 of the Probiotic Bacterium Lactobacillus reuteri 180

Microorganisms as potential corrosion inhibitors of metallic materials

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