Structural and Biochemical Characterization of a Polysaccharide Isolated From Vaccinium uliginosum L

Abstract

A novel polysaccharide (VUP-1) from Vaccinium uliginosum L. fruits is isolated using polyamide resin and Sephadex G-100 column chromatography. VUP-1 is a heteropolysaccharide, which is composed of mannose, galacturonic acid, galactose, glucose, and arabinose at a molar ratio of 1.00:3.35:7.99:8.38:11.89 with a molecular weight of 4.98 × 10 4 kDa. The glycosyl linkages of (1→5)-𝜶-L-Arabinose, (1→4)-𝜷-D-Galactose, (1→6)-𝜶-D-Galactose, (1→6)-𝜶-D-Glucose, and (1→4)-𝜷-D-Mannose are confirmed by nuclear magnetic resonance. Based on scanning electron microscopy and Congo red assays, VUP-1 shows flake-like structures, but no triple helix structure. VUP-1 presents high antioxidant activities (DPPH•, OH•, and reducing power) and moderate 𝜶-amylase inhibitory activity. Scavenging rate of VUP-1 on DPPH• and OH• are 93.86 ± 0.59% and 48.58 ± 0.64%, respectively. Reducing power of VUP-1 reached 1.14 ± 0.05. Inhibition rate of 𝜶-amylase is 37.23 ± 0.45% when VUP-1 concentration is 4.0 mg mL -1 . In addition, VUP-1 shows obvious inhibitory activities on the formation of dicarbonyl compounds (42.10±0.61%) and improved glycation end products (45.93±1.27%) compared to that of Amadori products (29.91±0.78%). This study concludes that polysaccharides from Vaccinium uliginosum L. have potential to be used as oral hypoglycemic agents.

Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake

“…The fruits of rabbiteye blueberry ( V. ashei ) accumulate galacturonic acid in polysaccharides (37.3 mol%) and to a lesser degree arabinose (29.7 mol%), glucose (15.5 mol%), and galactose (10.9 mol%) [ 50 ]. Glucose (41.0 mol%) and xylose (33.0 mol%) were the main monosaccharides of European blueberries ( V. myrtillus ) [ 53 ], whereas arabinose (36.4 mol%), glucose (25.7 mol%), and galactose (24.5 mol%) dominated in bog blueberries ( V. uliginosum ) [ 54 ]. Probably, arabinose, galactose, glucose, and galacturonic acid are essential monosaccharides of Vaccinium polysaccharides.…”