Structural and Cell-associated Proteins of Lassa Virus

Antigenic relatedness between arenaviruses defined at the epitope level by monoclonal antibodies

“…2). This a p p e a r a n c e of the N P cleavage fragment in R I P assays has been described in other reports which found that the Lassa virus N P in the infected cells was host cell-dependent (Clegg et al, 1983;Hufert et al, 1989). Those M A b s showing the diffuse I F A staining p a t t e r n reacted with either GP1 or G P 2 and also reacted with G P C (Fig.…”

“…Those M A b s showing the diffuse I F A staining p a t t e r n reacted with either GP1 or G P 2 and also reacted with G P C (Fig. 2), the intracellular precursor of glycoprotein present in E6-infected cells as described for arenaviruses (Clegg et al, 1983; C o m p a n s et al., 1985).…”

“…Some sequencing and genetic mapping of Old World arenaviruses has been carried out (Clegg et al, 1983;Gonzalez et al, 1984;Auperin et al, 1986). The development of monoclonal antibodies (MAbs) to lymphocytic choriomeningitis (LCM) virus has allowed exploration of antigenic relationships between certain Old World arenaviruses at the polypeptide level (Buchmeier et al, 1980(Buchmeier et al, , 1981(Buchmeier et al, , 1987Howard et al, 1985;Weber et al, 1988).…”

“…Thus the gene for the nucleocapsid protein (NP, 38K) is encoded at the 3' end of viral complementary RNA whereas those for the glycoproteins (GP1, 45K and GP2, 38K) are encoded at the 5' end of the viral RNA (Kiley et al, 1980). The glycoproteins are synthesized as a single precursor (GPC) and cleaved following translation (Clegg & Lloyd, 1983).…”

Localization of an Arenavirus Protein in the Nuclei of Infected Cells

“…4). Clegg & Lloyd (1983) have also identified similar protein species in Lassa virus-infected cells and suggested that cleavage fragments of N arose predominantly after detergent lysis and may therefore simply be artefacts of the preparative techniques employed. In our hands however, both Western blot and metabolic labelling experiments revealed the presence of the two polypeptides in Pichinde virus-infected cells harvested directly into PAGE disruption buffer.…”