Structural and dendroecological analysis of Quercus petraea subsp. austrothyrrenica Brullo, Guarino &amp; Siracusa in the Madonie Natural Park (Sicily)

Merlino, Angelo; Baliva, Michele; Filippo, Alfredo Di; Piovesan, Gianluca

doi:10.4129/2cis-am-ana

Cited by 3 publications

(3 citation statements)

References 9 publications

(9 reference statements)

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“…A similar picture holds for the diverging southern Q. robur population Pedro, where diagnosticity is substantially reduced at a few near-diagnostic SNPs in comparison to other Q. robur populations (Table 3), most likely due to introgression by Q. pubescens and Q. pyrenaica (Figure 8 in Appendix). Anecdotally the diverging status of Aspromonte, Pomieri and Pedro echoes with the taxonomic subspecies status that has been assigned to the Sicilian and Calabrian Q. petraea populations ( Q. petraea ssp austrothyrrenica , Bagnato et al , 2012; Lupini et al , 2019; Merlino et al , 2014) and to the extreme southern spanish Q. robur populations ( Q. robur ssp estremadurensis, Vazquez-Pardo et al , 2009).…”

Section: Discussionmentioning

confidence: 99%

Whole-genome screening for near-diagnostic genetic markers for white oak species identification in Europe

Kremer,

Delcamp,

Lesur

et al. 2023

Preprint

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ContextIdentifying species in the European white oak complex has been a long standing concern in taxonomy, evolution, forest research and management.Quercus petraea, Q. robur, Q. pubescensandQ. pyrenaicaare part of this species complex in western temperate Europe and hybridize in mixed stands, challenging species identification.AimsOur aim was to identify diagnostic single nucleotide polymorphisms (SNPs) for each of the four species that are suitable for routine use and rapid diagnosis in research and applied forestry.MethodsWe first scanned existing whole-genome and target-capture data sets in a reduced number of samples (training set) to identify candidate diagnostic SNPs, ie genomic positions being characterized by a reference allele in one species and by the alternative allele in all other species. Allele frequencies of the candidates SNPs were then explored in a larger, range-wide sample of populations in each species (validation step).ResultsWe found a subset of 38 SNPs (ten forQ. petraea, seven forQ. pubescens, nine forQ. pyrenaicaand twelve forQ. robur) that showed near-diagnostic features across their species distribution ranges withQ. pyrenaicaandQ. pubescensexhibiting the highest and lowest diagnosticity, respectively.ConclusionsWe provide a new, efficient and reliable molecular tool for the identification of the speciesQ. petraea, Q. robur, Q. pubescensandQ. pyrenaica, which can be used as a routine tool in forest research and management. This study highlights the resolution offered by whole-genome sequencing data to design diagnostic marker sets for taxonomic assignment, even for species complexes with relatively low differentiation.

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Section: Discussionmentioning

confidence: 99%

Whole-genome screening for near-diagnostic genetic markers for white oak species identification in Europe

Kremer,

Delcamp,

Lesur

et al. 2023

Preprint

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show abstract

“…Anecdotally, the diverging status of Aspromonte, Pomieri, and Pedro echoes with the taxonomic subspecies status that has been assigned to the Sicilian and Calabrian Q. petraea populations (Q. petraea ssp. austrothyrrenica, Bagnato et al 2012;Lupini et al 2019;Merlino et al 2014) and to the extreme southern spanish Q. robur populations (Q. robur ssp. estremadurensis, Vazquez-Pardo et al 2009).…”

Section: Variation Of Diagnosticity Among Snpsmentioning

confidence: 99%

Whole-genome screening for near-diagnostic genetic markers for four western European white oak species identification

Kremer,

Delcamp,

Lesur

et al. 2024

Annals of Forest Science

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Key message Mining genome-wide DNA sequences enabled the discovery of near-diagnostic markers for species assignment in four European white oaks (Quercus petraea (Matt.) Liebl., Quercus pubescens Willd., Quercus pyrenaica Willd., and Quercus robur L.) despite their low interspecific differentiation. Near-diagnostic markers are almost fully fixed in one species and absent in the three others. As a result, only a handful of markers are needed for species identification, making this genetic assay a very promising operational taxonomic assignment procedure in research and forestry. Context Identifying species in the European white oak complex has been a long-standing concern in taxonomy, evolution, forest research, and management. Quercus petraea (Matt.) Liebl., Q. robur L., Q. pubescens Willd., and Q. pyrenaica Willd. are part of this species complex in western temperate Europe and hybridize in mixed stands, challenging species identification. Aims Our aim was to identify near-diagnostic single-nucleotide polymorphisms (SNPs) for each of the four species that are suitable for routine use and rapid diagnosis in research and applied forestry. Methods We first scanned existing whole-genome and target-capture data sets in a reduced number of samples (training set) to identify candidate diagnostic SNPs, i.e., genomic positions being characterized by a reference allele in one species and by the alternative allele in all other species. Allele frequencies of the candidates SNPs were then explored in a larger, range-wide sample of populations in each species (validation step). Results We found a subset of 38 SNPs (10 for Q. petraea, 7 for Q. pubescens, 9 for Q. pyrenaica, and 12 for Q. robur) that showed near-diagnostic features across their species distribution ranges with Q. pyrenaica and Q. pubescens exhibiting the highest (0.876) and lowest (0.747) diagnosticity, respectively. Conclusions We provide a new, efficient, and reliable molecular tool for the identification of the species Q. petraea, Q. robur, Q. pubescens, and Q. pyrenaica, which can be used as a routine tool in forest research and management. This study highlights the resolution offered by whole-genome sequencing data to design near-diagnostic marker sets for taxonomic assignment, even for species complexes with relatively low differentiation.

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“…610 -Castelbuono, Italian Military Geographic Institute). One of the most interesting aspects of this forest plant community is its considerable size and the geographical location corresponding to the southernmost limit of the distribution area of durmast oak in Europe (Merlino & al. 2014).…”

Section: Study Areamentioning

confidence: 99%

A first contribution to the cryptogamic flora of “Bosco Pomieri” (Northern Sicily)

2020

Borziana

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Structural and dendroecological analysis of Quercus petraea subsp. austrothyrrenica Brullo, Guarino & Siracusa in the Madonie Natural Park (Sicily)

Cited by 3 publications

References 9 publications

Whole-genome screening for near-diagnostic genetic markers for white oak species identification in Europe

Whole-genome screening for near-diagnostic genetic markers for white oak species identification in Europe

Whole-genome screening for near-diagnostic genetic markers for four western European white oak species identification

A first contribution to the cryptogamic flora of “Bosco Pomieri” (Northern Sicily)

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