Structural and Functional Analysis of the Promoter of the Human α1(XI) Collagen Gene

Yoshioka, Hidekatsu; Greenwel, Patricia; Inoguchi, Kazuhito; Truter, Sharada L.; Inagaki, Yutaka; Ninomiya, Yoshifumi; Ramirez, Francesco

doi:10.1074/jbc.270.1.418

Cited by 29 publications

(46 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Culture-Human rhabdomyosarcoma cell line A204 (17,18) was used in this study. Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% heart-inactivated fetal bovine serum (Sanko Junyaku, Tokyo) at 37°C in humidified 5% CO 2 /95% air.…”

Section: Cells and Cellmentioning

confidence: 99%

“…It was expressed in mesenchymal cells, and its level of binding activity was positively correlated with the degree of cell differentiation in osteoblastic and skeletal muscle cell lines, in vitro. At Ϫ395 to Ϫ379, the core sequence of FP7 exhibits some homology with that of FPB 1 in COL5A2, and FP7-protein complexes can be competed with an excess of FPB oligonucleotides in an EMSA using nuclear extracts from the 1120 cell line (17,19). Further studies using transfection experiments and EMSAs in A204 cells, however, indicated that the FPB-protein complexes identified were PBX, PREP and HOX proteins and therefore different from the FP7-binding proteins (20).…”

mentioning

confidence: 99%

“…We have previously reported the structural and functional features of the human ␣1(XI) collagen gene (COL11A1) promoter (17). DNase I footprinting has mapped nine areas, FP1 to FP9, covering the region Ϫ541 to ϩ1, where nuclear proteins probably bind.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Matsuo¹,

Wang²,

Sumiyoshi³

et al. 2003

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

We have characterized the proximal promoter region of the human COL11A1 gene. Transient transfection assays indicate that the segment from ؊199 to ؉1 is necessary for the activation of basal transcription. Electrophoretic mobility shift assays (EMSAs) demonstrated that the ATTGG sequence, within the ؊147 to ؊121 fragment, is critical to bind nuclear proteins in the proximal COL11A1 promoter. We demonstrated that the CCAAT binding factor (CBF/NF-Y) bound to this region using an interference assay with consensus oligonucleotides and a supershift assay with specific antibodies in an EMSA. In a chromatin immunoprecipitation assay and EMSA using DNA-affinity-purified proteins, CBF/NF-Y proteins directly bound this region in vitro and in vivo. We also showed that four tandem copies of the CBF/NF-Ybinding fragment produced higher transcriptional activity than one or two copies, whereas the absence of a CBF/NF-Y-binding fragment suppressed the COL11A1 promoter activity. Furthermore, overexpression of a dominant-negative CBF-B/NF-YA subunit significantly inhibited promoter activity in both transient and stable cells. These results indicate that the CBF/NF-Y proteins regulate the transcription of COL11A1 by directly binding to the ATTGG sequence in the proximal promoter region.The collagen superfamily, one of the extracellular matrix proteins, plays an important role, not only in stabilizing the tissues as structural components but also in regulating a variety of biological functions, such as development, differentiation, proliferation, and morphogenesis (1-3). Among them, types I, II, III, V, and XI collagens are included in the group of fibril-forming collagens, based on their structural and functional features, and are divided into two subgroups, major (I, II and III) and minor (V and XI) fibrillar collagen (4) on the basis of their contents in tissues.Type XI collagen is a component of the collagen fibrillar network found in cartilage (5), and consists of three genetically distinct polypeptide chains: ␣1(XI), ␣2(XI), and ␣3(XI); the last of these is thought to be overglycosylated ␣1(II) chains (6).Although type XI collagen is a relatively minor collagen and is buried within the major collagen fibrils, it is important for the regulation of fibril diameter (7). Chondrodysplasia mice (cho), which do not synthesize ␣1(XI) chains, show irregular collagen fibrils in their cartilage (8). Contrary to the previous findings, the ␣1(XI) chain is not restricted to cartilage (9 -14), and it was demonstrated that the ␣1(XI) chain could form a heterotrimer with the ␣2(V) chain in a 2:1 ratio in non-cartilaginous cells and tissues (15,16). Although the precise function of this crosstype trimer remains unclear, the ␣1(XI) collagen gene is more broadly expressed than other collagen genes, showing that the ␣1(XI) collagen gene is the sole collagen gene to be expressed in both cartilaginous and non-cartilaginous tissues. This implies that the regulation of the ␣1(XI) collagen gene might be more complex than expected.We have previously re...

show abstract

Section: Cells and Cellmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Matsuo¹,

Wang²,

Sumiyoshi³

et al. 2003

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…This renders the 5Ј-flanking region of COL15A1 similar to promoters of the "housekeeping genes," which are transcribed widely but at low RNA levels in many tissues. Several other collagen genes also contain multiple GC boxes as their main promoter elements, including the COL5A1, COL7A1, COL11A1, and COL11A2 genes (26,(32)(33)(34). In addition, the downstream promoter of COL6A2 (35) and the upstream promoter of Col18a1 (14), two collagen genes with alternate promoters, are also of this kind.…”

Section: Intron Boundaries Of the Gene Coding For The Human ␣1(xv) Comentioning

confidence: 99%

Complete Exon-Intron Organization of the Human Gene for the α1 Chain of Type XV Collagen (COL15A1) and Comparison with the Homologous Col18a1 Gene

Hägg

Muona

Liétard

et al. 1998

Journal of Biological Chemistry

View full text Add to dashboard Cite

The human gene for the ␣1 chain of type XV collagen (COL15A1) is about 145 kilobases in size and contains 42 exons. The promoter is characterized by the lack of a TATAA motif and the presence of several Sp1 binding sites, some of which appeared to be functional in transfected HeLa cells. Comparison with Col18a1, which encodes the ␣1(XVIII) collagen chain homologous with ␣1(XV), indicates marked structural homology spread throughout the two genes. The mouse Col18a1 contains one exon more than COL15A1, due to the fact that COL15A1 lacks sequences corresponding to exon 3 of Col18a1, which encodes a cysteine-rich sequence motif. Twenty-five of the exons of the two genes are almost identical in size, six of them contain conserved split codons, and the locations of the respective exon-intron junctions are identical or almost identical in the two genes. The homologous exons include the closely adjacent first pair of exons and the exons encoding a thrombospondin-1 homology found in the N-terminal noncollagenous domain 1, which are followed by the most variable part of the two genes, covering the C-terminal half of their noncollagenous domain 1 and the beginning of the collagenous portion, after which most of the exons are homologous. The lengths of the introns are not similar in these genes, with two exceptions, namely the first intron, which is very short, less than 100 base pairs, and the second intron, which is very large, about 50 kilobases, in both genes. It can be concluded that COL15A1 and Col18a1 are derived from a common ancestor.

show abstract

“…COL11A is expressed mainly in articular cartilage and controls collagen type II (9,10). It functions in skeletal morphogenesis, fibrillogenesis, chondrocyte maturation and bone mineralization (11,12).…”

Section: Introductionmentioning

confidence: 99%

Association between collagen type XI α1 gene polymorphisms and papillary thyroid cancer in a Korean population

Choe

Kim

Park

et al. 2011

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Collagen type XI α1 (COL11A1) gene overexpression has been implicated as a candidate marker of various types of cancers. In this study, we investigated whether coding region single nucleotide polymorphisms (cSNPs) of the COL11A1 gene are associated with papillary thyroid cancer (PTC) in a Korean population. Four cSNPs [rs12731843 (Lys276Asn), rs3753841 (Pro1335Leu), rs1763347 (Gly1516Gly) and rs2229783 (Ile1602Ile)] were genotyped using direct sequencing in 98 PTC patients and 366 control subjects. Logistic regression analysis for each cSNP revealed an association between rs1763347 and PTC in a dominant model [CT/TT vs. CC, p= 0.0042, odds ratio (OR)= 0.50, 95% confidential interval (CI) 0.31-0.81]. Analysis of allelic frequency showed that the T alleles of rs1763347 and rs2229783 were significantly associated with reduced risk of PTC (p= 0.010, OR=0.61, 95% CI 0.42-0.89 in rs1763347; p=0.007, OR=0.62, 95% CI 0.44-0.88 in rs2229783). Additionally, in the analysis of haplotype, the CC haplotype consisting of rs1763347 and rs2229783 was associated with PTC in codominant (p=0.011, OR=1.56, 95% CI 1.11-2.21) and recessive models (p=0.020, OR=1.70, 95% CI 1.09-2.66). The TT haplotype was also associated with PTC in a codominant model (p=0.006, OR=0.58, 95% CI 0.39-0.88). The frequency of the CC haplotype was higher in the PTC patients (0.71) compared to the control subjects (0.61), whereas the frequency of the TT haplotype was lower in the PTC patients (0.20 and 0.30 in PTC patients and control subjects, respectively). The results suggest that the COL11A1 gene may be associated with PTC and, in particular, that the T allele of rs1763347 and rs2229783 may contribute to a reduced risk of PTC.

show abstract

Structural and Functional Analysis of the Promoter of the Human α1(XI) Collagen Gene

Cited by 29 publications

References 36 publications

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Complete Exon-Intron Organization of the Human Gene for the α1 Chain of Type XV Collagen (COL15A1) and Comparison with the Homologous Col18a1 Gene

Association between collagen type XI α1 gene polymorphisms and papillary thyroid cancer in a Korean population

Contact Info

Product

Resources

About