Structural and functional analysis show that the <i>Escherichia coli</i> uncharacterized protein <scp>Y</scp>jc<scp>S</scp> is likely an alkylsulfatase

Liang, Yajing; Gao, Zengqiang; Dong, Yuhui; Liu, Quansheng

doi:10.1002/pro.2528

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…The third group includes enzymes with the metallo-β-lactamase-like domain in the N-terminus and SCP-2-like domain in C-terminus. Six sulfatases with different substrate specificities have been characterized to date: SdsA, YraS, and YjcS with activity toward sodium dodecyl sulfate (SDS) ( Davison et al, 1992 ; Liang et al, 2014 ; Navais et al, 2014 ), SdsAP and SdsA1 with activity toward primary sulfate esters including sodium dodecyl sulfate ( Hagelueken et al, 2006 ; Long et al, 2011 ; Schober et al, 2011 ) and Pisa1 with activity toward secondary alkyl sulfates ( Schober et al, 2011 ). Enzymes from this group differ in the mechanism of action and could cleave either S-O or C-O bond causing the retention or inversion of the product alcohol, which could be verified using chiral or labeled substrates.…”

Section: Introductionmentioning

confidence: 99%

“…However, they are mainly focused on the phylogenetic identification of the isolates and the alkylsulfatase activity testing using Native Page Zymography without the detailed enzyme characteristics. Therefore, there are only five well-characterized alkylsulfatases with known sequence and confirmed activity toward SDS ( Davison et al, 1992 ; Hagelueken et al, 2006 ; Long et al, 2011 ; Liang et al, 2014 ; Navais et al, 2014 ). This year a first attempt to identify the enzymes involved in the further SDS metabolic pathway in P. aeruginosa PAO1 was performed.…”

Section: Introductionmentioning

confidence: 99%

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Genomic and Functional Characterization of Environmental Strains of SDS-Degrading Pseudomonas spp., Providing a Source of New Sulfatases

et al. 2018

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Biochemical, physiological and genomic comparisons of two Pseudomonas strains, assigned previously to the Pseudomonas jessenii subgroup, which are efficient SDS-degraders were carried out. A GO enrichment analysis showed that the genomes of SDS-degraders encode more genes connected with bacterial cell wall biosynthesis and alkanesulfonate monooxygenase activity than their closest relatives from the P. jessenii subgroup. A transcriptomic analysis of the most promising strain exposed to detergent suggests that although SDS can be later utilized as a carbon source, in early stages it influences cell envelope integrity, causing a global stress response followed by cell wall modification and induction of repair mechanisms. Genomes of the analyzed strains from P. jessenii group encode multiple putative sulfatases and their enzymatic activity was experimentally verified, which led to the identification of three novel enzymes exhibiting activity toward SDS. Two of the novel alkylsulfatases showed their highest activity at pH 8.0 and the temperature of 60°C or 70°C. One of the enzymes retained its activity even after 1 h of incubation at 60°C. Ions like K+ and Mg2+ enhanced enzymatic activity of both proteins, whereas Cu2+ or EDTA had inhibitory effects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic and Functional Characterization of Environmental Strains of SDS-Degrading Pseudomonas spp., Providing a Source of New Sulfatases

et al. 2018

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“…The second gene, yjcS (EcSMS35_1714 in E. coli ), is an alkyl-sulfatase. This enzyme has been first described in Pseudomonas spp ., where a strain carrying this enzyme was able to grow on the surfactant sodium dodecyl sulphate (SDS), and the gene has been characterised in E. coli as well 30,31 .…”

Section: Resultsmentioning

confidence: 99%

Multi-layered networks of SalmoNet2 enable strain comparisons of the Salmonella genus on a molecular level

Ölbei

Bohár

Fazekas

et al. 2021

Preprint

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Serovars of the genus Salmonella primarily evolved as gastrointestinal pathogens in a wide range of hosts. Some serotypes later evolved further, adopting a more invasive lifestyle in a narrower host range associated with systemic infections. A system-level knowledge of these pathogens has the potential to identify the complex adaptations associated with the evolution of serovars with distinct pathogenicity, host range and risk to human health. This promises to aid the design of interventions and serve as a knowledge base in the Salmonella research community. Here we present SalmoNet2, a major update to SalmoNet, the first multi-layered interaction resource for Salmonella strains, containing protein-protein, transcriptional regulatory and enzyme-enzyme interactions. The new version extends the number of Salmonella genomes from 11 to 20, including strains such as Salmonella Typhimurium D23580, an epidemic multidrug-resistant strain leading to invasive non-typhoidal Salmonella Disease (iNTS), and a strain from Salmonella bongori, another species in the Salmonella genus. The database now uses strain specific metabolic models instead of a generalised model to highlight differences between strains. This has increased the coverage of high-quality protein-protein interactions, and enhances interoperability with other computational resources by adopting standardised formats. The resource website has been updated with tutorials to help researchers analyse their Salmonella data using molecular interaction networks from SalmoNet2. SalmoNet2 is accessible at http://salmonet.org/.</SPAN>

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“…Alkylsulfatase activity was measured by the rate of phenol red oxidation at 557 nm ( ε 557 = 44,100 M −1 cm −1 ) (Liang et al 2014 ). The measurements were made in 96-well plates, and the absorbance decrease was read on a Spark 10 M tablet spectrophotometer (Tecan, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Whole-cell electric sensor for determination of sodium dodecyl sulfate

Velichko

Guliy

Kanevsky

et al. 2022

World J Microbiol Biotechnol

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Linear alkyl sulfates are a major class of surfactants that have large-scale industrial application and thus wide environmental release. These organic pollutants threaten aquatic environments and other environmental compartments. We show the promise of the use of a whole-cell electric sensor in the analysis of low or residual concentrations of sodium dodecyl sulfate (SDS) in aqueous solutions. On the basis of bioinformatic analysis and alkylsulfatase activity determinations, we chose the gram-negative bacterium Herbaspirillum lusitanum , strain P6–12, as the sensing element. Strain P6–12 could utilize 0.01–400 mg/L of SDS as a growth substrate. The electric polarizability of cell suspensions changed at all frequencies used (50–3000 kHz). The determination limit of 0.01 mg/L is much lower than the official requirements for the content of SDS in potable and process water (0.5 and 1.0 mg/L, respectively), and the analysis takes about 1–5 min. The promise of H. lusitanum P6–12 for use in the remediation of SDS-polluted soils is discussed. Graphical abstract

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Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase

Cited by 8 publications

References 40 publications

Genomic and Functional Characterization of Environmental Strains of SDS-Degrading Pseudomonas spp., Providing a Source of New Sulfatases

Genomic and Functional Characterization of Environmental Strains of SDS-Degrading Pseudomonas spp., Providing a Source of New Sulfatases

Multi-layered networks of SalmoNet2 enable strain comparisons of the Salmonella genus on a molecular level

Whole-cell electric sensor for determination of sodium dodecyl sulfate

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