Structural and functional characteristics of muscle fibres in pigs with different malignant hyperthermia susceptibility (MHS) and different meat quality

Fiedler, I.; Ender, K.; Wicke, Michael; Maak, Steffen; Lengerken, G. von; Meyer, W

doi:10.1016/s0309-1740(99)00030-3

Cited by 79 publications

(50 citation statements)

References 28 publications

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“…In accordance with Rahelic and Puac (1980) we observed giant fibres in the Ld muscle that is a white muscle in which fast-twitch glycolytic fibres prevail in number. The ''giant fibre" phenomenon is known to occur in skeletal muscle samples obtained after slaughter and has been described in pigs of different breeds (Fiedler et al 1999;Handel and Stickland 1986) and confirmed in the study of Schubert-Schoppmeyer et al (2008). Our study shows that there are differences between males and females with regard to the size of all three fibre types; namely, the males showed larger fibres than the females (Figs 5 and 6).…”

Section: Discussionsupporting

confidence: 83%

New Insights into Muscle Fibre Types in Casertana Pig

et al. 2010

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Little is known about the Casertana pig. The aim of this study was to evaluate the effect of sex on histochemical and morphometrical characteristics of muscle fibres (myocytes) in this pure breed and to verify the presence of giant fibres as well as vascularity of the muscle. Finally, maximum shortening velocity and isometric tension were measured in single muscle fibres.Sixteen Casertana pigs (8 males, 8 females) from a farm in Campania (Italy) were slaughtered at one year of age. Muscle tissues were obtained from psoas minor, rhomboideus and longissimus dorsi. Myofibres were stained for myosin adenosine triphosphatase, succinic dehydrogenase, and α-amylase-periodic acid schiff. For all fibre types, the area and perimeter were measured. Slowtwitch oxidative fibres, fast-twitch glycolytic fibres and fast-twitch oxidative-glycolytic fibres were histochemically differentiated; an image-analyzing system was used. The results showed significant differences between the sexes in the size of all three fibre types. The psoas minor muscle had a high percentage of slow-twitch oxidative fibres and contained more capillaries per fibre and per mm 2 than rhomboideus and longissimus dorsi, in which fast-twitch glycolytic fibres dominated. The cross-sectional area of all fibre types was larger in longissimus dorsi than in rhomboideus and psoas minor muscles; the giant fibres were present in the longissimus dorsi muscle only. Besides, isometric tension values were higher in fast-twitch glycolytic fibres than in the other ones. Variations in fibre type composition may contribute to meat quality. Giant fibres, myosin adenosine triphosphatase, succinic dehydrogenase, capillaries, shortening velocity, isometric tensionThe Casertana breed is an autochthonous pig breed of ancient origin, now threatened with extinction. In the past it was considered a very fine breed, raised in the Campania region and the surrounding area in South Italy. It was valued for its productive performance particularly regarding the fattening tendency. It is characterized by slow growth and massive accumulation of backfat; its coat is bright black and hairless. The purpose of this study was to complete the knowledge regarding the Casertana pig in order to valorise this breed and to study its meat quality characteristics. Porcine meat quality can be affected by variation in muscle fibre type composition (Depreux 2000). Muscle fibre types may be classified based on enzymatic activity; in this study the following nomenclature was used for myofibre types: FG (fast-contracting with glycolytic metabolism), FOG (fast contracting with glycolytic-oxidative metabolism) and SO (slow-contracting with oxidative metabolism). The aim of this study was to evaluate the effect of sex on the histochemical and morphometric characteristics of muscle fibres on the basis of myosin adenosine triphosphatase (myosin ATPase), succinic dehydrogenase (SDH), to analyse the contractile properties of pig muscle fibres and to determine the presence of giant fibres in the muscles consi...

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Section: Discussionsupporting

confidence: 83%

New Insights into Muscle Fibre Types in Casertana Pig

et al. 2010

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“…Literature indicates that higher meat content in carcass and worse meat quality in crossbreds descending from Pietrain boars may be due to a high frequency of the T allele at the RYR1 gene. Differences in the quality of carcass and particularly in meat quality between breeds or crossbreds are connected primarily with the presence of a varying frequency of RYR1 genotypes, as documented by the results of other reports (Larzul et al, 1997;Fiedler et al, 1999). For example, Biedermann et al (2000) showed that in porkers descending from Pietrain boars, a decreasing share of the T allele is followed by reduced carcass value, improved meat quality, and increased content of intramuscular fat.…”

Section: Resultsmentioning

confidence: 94%

Carcass and meat quality traits of pig reciprocal crosses with a share of Pietrain breed

Rybarczyk¹,

Pietruszka²,

Jacyno³

et al. 2011

CZECH J ANIM SCI

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AbstrACt:The study was carried out on 119 hybrid grower-finisher pigs that were divided into 5 experimental groups. The analysis covered carcass and meat quality traits of crossbred pigs produced by mating Polish Large White × Polish Landrace crossbred sows with boars of either purebred Pietrain (P) or crossbreds of Line 990 × Pietrain (LP), or Pietrain × Line 990 (PL), or Duroc × Pietrain (DP), or Pietrain × Duroc (PD). The analysis of results which include the effect of the RYR1 gene polymorphism showed that porkers descending from Pietrain boars (P) had a higher meat content in the carcass and a larger loin eye area in comparison with porkers descending from DP and PD boars, and a larger loin eye area in comparison with porkers descending from boars that were LP crosses. Meat of porkers descending from Pietrain boars had lower water-holding capacity (WHC) and higher lightness (L*) in comparison with porkers descending from crossbred boars where the Pietrain breed was used on the paternal side (LP and DP). Meat of porkers descending from Pietrain boars had a lower content of water-soluble protein compared to other groups with a 25% share of the Pietrain breed. In addition, no significant influence of the position effect on carcass and meat quality was found out in porkers obtained from the crossing of sows of the White breed with crossbred boars descending from two-way crosses of the Pietrain breed with Line 990 (LP and PL) and with the Duroc breed (DP and PD).

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“…Essen-Gustavsson et al (1992) and Fiedler et al (1999) demonstrated that the fibre diameter in m. longissimus dorsi was larger in pigs susceptible to stress, when compared to that observed in stress-resistant animals. In turn, Gallant (1980) and Ackermann and Salomon (1991) did not observe such a relation.…”

Section: Effect Of Ryr1 Genotype On Microstructural Characteristics Omentioning

confidence: 96%

A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses

Kłosowska¹,

Kurył²,

Elminowska-Wenda³

et al. 2004

CZECH J ANIM SCI

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Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied between MYOD1, myogenin and MYF5 genotypes and microstructural characteristics of the m. longissimus lumborum in pigs – crosses: Pietrain × (Polish Large White × Polish Landrace). The data included 115 unrelated animals slaughtered at about 105 kg live body weight. Within 45 min after exsanguination, samples were taken from the m. longissimus lumborum, frozen in liquid nitrogen and later analysed for the diameter of slow-twitch oxidative, fast-twitch oxidative and fast-twitch low-oxidative fibres, their proportion in a bundle, the proportion of pathological changes and number of fibres per unit area. The RYR1 and MyoD genotypes were determined using the PCR-RFLP technique. A significant or highly significant relation was observed between the diameter of all types of muscle fibres and genotype RYR1 – the highest values were recorded for homozygotes TT (genetically stress-sensitive). A relation between MyoD genotypes and microstructural characteristics of the m. longissimus lumborum was analysed on a group of 93 animals of the genotype CC or CT at locus RYR1. Sex appeared to have no significant effect on the muscle microstructural traits in this group of animals. The content of fast-twitch oxidative fibres (FTO) was significantly related to the MYF5 genotype, whereas that of fast-twitch low-oxidative fibres (FT) was affected by the MYOD1 and MYF5/DdeI genotypes. The proportion of angular fibres in a bundle was related to MYF5/HinfI genotype. The results showed that MyoD genes could be considered as candidate genes for some microstructural characteristics of m. longissimus lumborum in pigs.  

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Structural and functional characteristics of muscle fibres in pigs with different malignant hyperthermia susceptibility (MHS) and different meat quality

Cited by 79 publications

References 28 publications

New Insights into Muscle Fibre Types in Casertana Pig

New Insights into Muscle Fibre Types in Casertana Pig

Carcass and meat quality traits of pig reciprocal crosses with a share of Pietrain breed

A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses

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