Structural and Functional Characteristics of the Proline-Rich Antimicrobial Peptide Minibactenecin from Leukocytes of Domestic Goat Capra hircus

Bolosov, Ilia A.; Panteleev, Pavel V.; Balandin, Sergey V.; Shamova, Olga; Ovchinnikova, Tatiana V.

doi:10.1007/s10517-023-05725-1

Cited by 1 publication

(3 citation statements)

References 15 publications

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“…Similar to our previous experiments with other short PrAMPs such as VicBac 1-22 and a panel of mini-ChBac7.5Nα analogs [3,15,26], ≥32-fold increases in MIC values were registered just after initial 3-4 passages subjected to selection by Bac7 1-22 . The obtained Bac7 1-22 -resistant strains were named as B1 and B2.…”

Section: Induction Of Bacterial Resistancesupporting

confidence: 87%

“…All of the three Bac7 1-22 -resistant strains (B1-B3) showed similar growth rates in the rich LB medium (Figure 2A) that pointed at insufficient fitness costs of acquired mutations. Similar to our previous experiments with other short PrAMPs such as VicBac1-22 and a panel of mini-ChBac7.5Nα analogs [3,15,26], ≥32-fold increases in MIC values were registered just after initial 3-4 passages subjected to selection by Bac71-22. The obtained Bac71-22-resistant strains were named as B1 and B2.…”

Section: Induction Of Bacterial Resistancesupporting

confidence: 87%

“…The SbmA transporter is a mutation-prone protein undergoing a strong selective pressure when short PrAMPs are used to induce bacterial resistance in vitro. Previously, different ways of the SbmA inactivation (insertions, deletions, single nucleotide mutations) have already been observed in salt-containing media for a number of mammalian PrAMPs and their analogs [3,15,26,32]. In another study, a 600 bp insertion (producing a stop codon) was identified in the sbmA gene after serial passages of the E. coli BL21AI strain in the diluted TSB medium in the presence of known insect PrAMP apidaecin 1b [33].…”

Section: Resistance To Bac7 1-22 In the Salt-containing Medium Is Med...mentioning

confidence: 95%

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Genomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7

et al. 2023

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Proline-rich antimicrobial peptides (PrAMPs) having a potent antimicrobial activity and a modest toxicity toward mammalian cells attract much attention as new templates for the development of antibiotic drugs. However, a comprehensive understanding of mechanisms of bacterial resistance development to PrAMPs is necessary before their clinical application. In this study, development of the resistance to the proline-rich bovine cathelicidin Bac71-22 derivative was characterized in the multidrug-resistant Escherichia coli clinical isolate causing the urinary tract infection. Three Bac71-22-resistant strains with ≥16-fold increase in minimal inhibitory concentrations (MICs) were selected by serially passaging after four-week experimental evolution. It was shown that in salt-containing medium, the resistance was mediated by inactivation of the SbmA transporter. The absence of salt in the selection media affected both dynamics and main molecular targets under selective pressure: a point mutation leading to the amino acid substitution N159H in the WaaP kinase responsible for heptose I phosphorylation in the LPS structure was also found. This mutation led to a phenotype with a decreased susceptibility to both the Bac71-22 and polymyxin B. Screening of antimicrobial activities with the use of a wide panel of known AMPs, including the human cathelicidin LL-37 and conventional antibiotics, against selected strains indicated no significant cross-resistance effects.

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Section: Induction Of Bacterial Resistancesupporting

confidence: 87%

Section: Induction Of Bacterial Resistancesupporting

confidence: 87%

Section: Resistance To Bac7 1-22 In the Salt-containing Medium Is Med...mentioning

confidence: 95%

See 1 more Smart Citation

Genomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7

et al. 2023

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Structural and Functional Characteristics of the Proline-Rich Antimicrobial Peptide Minibactenecin from Leukocytes of Domestic Goat Capra hircus

Cited by 1 publication

References 15 publications

Genomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7

Genomic Insights into Bacterial Resistance to Proline-Rich Antimicrobial Peptide Bac7

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