Structural and functional characterization of the zebrafish lamin B receptor

Schild-Prüfert, Kristina; Giegerich, Marieke; Schäfer, Matthias; Winkler, Christoph; Krohne, Georg

doi:10.1016/j.ejcb.2006.04.009

Cited by 5 publications

(4 citation statements)

References 40 publications

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“…Similar results were shown for lamin B receptor (LBR), inner nuclear membrane protein responsible for heterochromatin positioning at nuclear periphery, present in NE, and membrane invaginations inside the cell nucleus (Ellenberg et al 1997 ). Knockdown of LBR by morpholino antisense nucleotides in 1–2 cell zebrafish embryos caused reduced viability and severe morphological alterations in surviving embryos (Schild-Prufert et al 2006 ). RNAi-mediated knockdown of LBR in HeLa cells resulted in failure of NE reassembly and abnormal chromatin decondensation after mitosis leading to the apoptotic cell death (Lu et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

et al. 2015

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Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.Electronic supplementary materialThe online version of this article (doi:10.1007/s00709-015-0861-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

et al. 2015

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“…However, the role of the LBR in neural progenitor cells has not been explored. Loss of the LBR in zebrafish led to severe developmental defects ( 34 ), which precluded further analysis.…”

Section: Resultsmentioning

confidence: 99%

Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

Xing

Chai²,

Wang³

et al. 2022

Journal of Biological Chemistry

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“…The lamin B receptor (LBR) is the most thoroughly studied protein of the RS domain‐containing proteins. The zebrafish LBR is the only characterized LBR in the fish group that contains three RS repeats neighboring an SP motif in exon 2, but the total number of RS/SR motifs is less than that in human LBRs [41]. LBR self‐associates through its N‐terminal domain and forms oligomers that are suggested to bind heterochromatin [42].…”

Section: Discussionmentioning

confidence: 99%

“…staining, 7.5% for Western blotting), except one-50th quantity of His-gfSRPK1 protein was loaded for Western blotting. neighboring an SP motif in exon 2, but the total number of RS/SR motifs is less than that in human LBRs [41]. LBR self-associates through its N-terminal domain and forms oligomers that are suggested to bind heterochromatin [42].…”

Section: Identification Of Rs/sr Dipeptide Repeats In Fish Lamin B3mentioning

confidence: 99%

In vitro characterization of the RS motif in N‐terminal head domain of goldfish germinal vesicle lamin B3 necessary for phosphorylation of the p34cdc2 target serine by SRPK1

et al. 2013

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The nuclear envelopes surrounding the oocyte germinal vesicles of lower vertebrates (fish and frog) are supported by the lamina, which consists of the protein lamin B3 encoded by a gene found also in birds but lost in the lineage leading to mammals. Like other members of the lamin family, goldfish lamin B3 (gfLB3) contains two putative consensus phosphoacceptor p34cdc2 sites (Ser-28 and Ser-398) for the M-phase kinase to regulate lamin polymerization on the N- and C-terminal regions flanking a central rod domain. Partial phosphorylation of gfLB3 occurs on Ser-28 in the N-terminal head domain in immature oocytes prior to germinal vesicle breakdown, which suggests continual rearrangement of lamins by a novel lamin kinase in fish oocytes. We applied the expression-screening method to isolate lamin kinases by using phosphorylation site Ser-28-specific monoclonal antibody and a vector encoding substrate peptides from a goldfish ovarian cDNA library. As a result, SRPK1 was screened as a prominent lamin kinase candidate. The gfLB3 has a short stretch of the RS repeats (9-SRASTVRSSRRS-20) upstream of the Ser-28, within the N-terminal head. This stretch of repeats is conserved among fish lamin B3 but is not found in other lamins. In vitro phosphorylation studies and GST-pull down assay revealed that SRPK1 bound to the region of sequential RS repeats (9–20) with affinity and recruited serine into the active site by a grab-and-pull manner. These results indicate SRPK1 may phosphorylate the p34cdc2 site in the N-terminal head of GV-lamin B3 at the RS motifs, which have the general property of aggregation.

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Structural and functional characterization of the zebrafish lamin B receptor

Cited by 5 publications

References 40 publications

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Expression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain

In vitro characterization of the RS motif in N‐terminal head domain of goldfish germinal vesicle lamin B3 necessary for phosphorylation of the p34cdc2 target serine by SRPK1

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