Structural and functional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii

Yoon, Sangyoung; Lee, Won Kyu; Kim, Min‐Soo; Kim, T. Doohun; Ryu, Yeon‐Woo

doi:10.1007/s00449-011-0640-x

Cited by 10 publications

(4 citation statements)

References 33 publications

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“…Cysteine desulfurylase (spot 33), which catalyzes the formation of L-alanine from the substrate L-cysteine, is involved in the syntheses of many kinds of sulfur-containing biomolecules (Chen et al, 2012). S-adenosylmethionine synthetase [spot 30 (known as methionine adenosyltransferase)] is considered the rate-limiting step of the methionine cycle (Markham and Pajares, 2009) and catalyzes the formation of Sadenosylmethionine, which has been suggested to be essential for gene transcription, cell proliferation, and production of secondary metabolites (Yoon et al, 2012). Although it is well known that phenylalanine is required as a precursor for anthocyanins, there is little information on the effect of the amino acid metabolism on anthocyanin synthesis.…”

Section: Resultsmentioning

confidence: 99%

Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

Niu

et al. 2013

J. Amer. Soc. Hort. Sci.

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The most obvious effects of a low leaf:fruit (LF) ratio [two leaves for one cluster per shoot (LF2)] on grape (Vitis vinifera) berries are suppressed anthocyanin biosynthesis in the berry skin, decreased berry weight and soluble solids concentration, and increased titratable acidity. In this study, proteins isolated from berry skins grown under low and high LF ratio conditions, LF2 and LF12, respectively, were characterized by two-dimensional gel electrophoresis coupled to mass spectrometry. A survey of ≈600 to 700 spots from berry skin yielded 77 proteins with differential expression between LF12 and LF2 treatments. Of these, the 59 proteins that were identified consisted of 47 proteins that were down-regulated and 12 that were up-regulated under LF2 conditions compared with LF12 conditions. Most proteins involved in metabolism, energy, transcription, protein synthesis, binding function, signal transduction, and cell defense were down-regulated in LF2 berries, whereas two important enzymes of anthocyanin biosynthesis, chalcone synthase and dihydroflavonol reductase, were not detected. Only a few proteins (e.g., two heat shock proteins related to protein fate and nutrient reservoir storage protein) were found to be up-regulated in LF2 berries. This suggested that, with the exception of secondary metabolism, many proteomic events may have an effect on anthocyanin synthesis in the skins responding to LF.

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Section: Resultsmentioning

confidence: 99%

Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

Niu

et al. 2013

J. Amer. Soc. Hort. Sci.

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“…AdoMet metabolism is complicated by its interaction with plant growth hormones such as cytokinins and auxins [ 27 ]. Thus, AdoMet is involved in regulating plant developmental by fine-tuning gene transcription, cell proliferation, and the production of secondary metabolites [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids

Yongtian²,

Zhao

et al. 2015

PLoS ONE

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A long period of silk viability is critical for a good seed setting rate in maize (Zea mays L.), especially for inbred lines and hybrids with a long interval between anthesis and silking. To explore the molecular mechanism of silk viability and its heterosis, three inbred lines with different silk viability characteristics (Xun928, Lx9801, and Zong3) and their two hybrids (Xun928×Zong3 and Lx9801×Zong3) were analyzed at different developmental stages by a proteomic method. The differentially accumulated proteins were identified by mass spectrometry and classified into metabolism, protein biosynthesis and folding, signal transduction and hormone homeostasis, stress and defense responses, and cellular processes. Proteins involved in nutrient (methionine) and energy (ATP) supply, which support the pollen tube growth in the silk, were important for silk viability and its heterosis. The additive and dominant effects at a single locus, as well as complex epistatic interactions at two or more loci in metabolic pathways, were the primary contributors for mid-parent heterosis of silk viability. Additionally, the proteins involved in the metabolism of anthocyanins, which indirectly negatively regulate local hormone accumulation, were also important for the mid-parent heterosis of silk viability. These results also might imply the developmental dependence of heterosis, because many of the differentially accumulated proteins made distinct contributions to the heterosis of silk viability at specific developmental stages.

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“…SAM acts as a transmethylator (transfers methyl groups from one molecule to another within a cell) or is involved in major biochemical reactions, such as gene transcription and expression, signaling, cell division and growth, and response to stress. Previous studies have also shown that overexpression of the SAM synthase gene or SAM-related genes in plants or eukaryotes increases tolerance to abiotic stress [ 14 - 20 ]. Therefore, in this study, we constructed and cultivated recombinant C. reinhardtii to overexpress the SAMS gene and investigate the cell growth and intracellular lipid accumulation under nitrogen-depleted or saline-stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production

Kim

Ahn

Park

et al. 2023

J. Microbiol. Biotechnol.

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Microalgae are attracting much attention as promising, eco-friendly producers of bioenergy due to their fast growth, absorption of carbon dioxide from the atmosphere, and production capacity in wastewater and salt water. However, microalgae can only accumulate large quantities of lipid in abiotic stress, which reduces productivity by decreasing cell growth. In this study, the strategy was investigated to increase cell viability and lipid production by overexpressing S-adenosylmethionine (SAM) synthetase (SAMS) in the microalga Chlamydomonas reinhardtii . SAM is a substance that plays an important role in various intracellular biochemical reactions, such as cell proliferation and stress response, and the overexpression of SAMS could allow cells to withstand the abiotic stress and increase productivity. Compared to wild-type C. reinhardtii , recombinant cells overexpressing SAMS grew 1.56-fold faster and produced 1.51-fold more lipids in a nitrogen-depleted medium. Furthermore, under saline-stress conditions, the survival rate and lipid accumulation were 1.56 and 2.04 times higher in the SAMS-overexpressing strain, respectively. These results suggest that the overexpression of SAMS in recombinant C. reinhardtii has high potential in the industrial-scale production of biofuels and various other high-value-added materials.

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Structural and functional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii

Cited by 10 publications

References 33 publications

Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins

Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids

Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production

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