Structural and functional differences of Litopenaeus vannamei crustins

Vargas‐Albores, Francisco; Yépiz-Plascencia, Glória; Jiménez-Vega, Florinda; Ávila-Villa, Angélica

doi:10.1016/j.cbpc.2004.05.007

Cited by 107 publications

(47 citation statements)

References 52 publications

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“…This observation is in agreement with the earlier work of Sun et al (2008) where a down-regulation of lectins (another group of AMPs) has been reported 24 h post-challenge WSSV in F. chinensis. Vargas-Albores et al (2004) and Okumura (2007) have also reported a decrease in the number of crustin transcripts when infected with Vibrio in L. vannamei. Chiou et al (2005) have reported no up-regulation of AMPs in the haemocytes of tiger shrimps when challenged with Vibrio.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

et al. 2011

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a b s t r a c tA crustin-like antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence of this crustin-like AMP from other shrimp crustins. The differential expression of the crustin-like AMP in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts (Candida haemulonii S27 and Candida sake S165) and two ␤-glucan isolates (extracted from C. haemulonii S27 and C. sake S165) were noted during the study. Responses to the application of two gram-positive probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile was recorded pre-and post-challenge white spot syndrome virus (WSSV) by semi-quantitative RT-PCR. Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in the experimental animals. The crustin-like AMP was found to be constitutively expressed in the animal and a significant down-regulation could be noted post-challenge WSSV. Remarkable down-regulation of the gene was observed in the immunostimulant fed animals pre-challenge followed by a significant up-regulation post-challenge WSSV. Tissue-wise expression of crustin-like AMP on administration of C. haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii and the probiotic bacteria, Bacillus were found to enhance the production of crustin-like AMP and confer significant protection to P. monodon against WSSV infection.

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Section: Discussionmentioning

confidence: 99%

“…Until recently, a few sequences of crustins have been described in penaeid shrimps (Gross et al 2001;Bartlett et al 2002;Vargas-Albores et al 2004;Rattanachai et al 2004;Zhang et al 2007). As AMPs play an important role in shrimp defense, the expression levels of these molecules are possible indicators of the immune state of shrimps.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

et al. 2011

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“…They bind to molecules within the host immune system, the pattern recognition receptors (PRRs), to form a complex that then initiates a series of events, such as signal transduction or activation of enzyme cascades that result in the liberation of bioactive molecules. What actually happens when protostome invertebrates are injected with PAMPs is that a full-blown immune response occurs, involving activation of particular enzyme cascades, such as the prophenoloxidase system, the regulated exocytosis of aggressive molecules, (4,13) an increase in the expression of genes coding for antimicrobial proteins (17,20,23) and /or other related proteins (see Table 1 and Smith et al (33) ), and the initiation of encapsulation reactions leading to haemocytopenia. Experiments in which presentation of PAMPs to the protostome immune system has been claimed to 'prime' the immune system, measure not immune parameters, as might be expected, but survival, (5) temporary increases in growth (34,35) or reproductive capacity.…”

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confidence: 99%

Adaptive immunity in invertebrates: A straw house without a mechanistic foundation

2007

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SummaryRecently claims have been made for radical new insights in the field of invertebrate immunology that involve memory, specificity and/or maternal transfer of immunocompetence. For evidence these claims rely on phenomena, such as survival or reproductive capacity, observed at the level of the whole organism. The allure of these apparently revelatory hypotheses is that they are contrary to established views of innate immunity. They draw implicit analogy to adaptive responses in jawed vertebrates and the terminology used creates an incomplete and misleading picture. We argue that the case for adaptive immunity in invertebrates based only on such phenomena is weak and flawed, as it can only be upheld if supported by descriptions of the underlying mechanisms. We caution against a reliance on this approach as a means of advancing this field-highlighting, as an example, some negative commercial implications of adopting this approach.

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“…Type 1 crustins, which phylogenetically group within the Pleocyemata and have a cysteine rich region positioned upstream of the WAP domain, and Type 2 crustins from shrimp that have an additional glycine rich domain 5'-of the cysteine rich region . Isoform variants are also recognised within this class structure (Vargas-Albores et al, 2004;Brockton et al, 2007). All full-length crustins have a signal sequence at the amino terminus indicating that, once produced within the granular haemocytes, they are secreted in to the plasma where they appear constitutively at high titre.…”

Section: Stored and Secreted Antimicrobial Peptidesmentioning

confidence: 99%

The scope of the crustacean immune system for disease control

Hauton

2012

Journal of Invertebrate Pathology

144

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The culture or wild capture of marine and freshwater shellfish, including crustaceans, is without doubt a key source of protein for a burgeoning world population. Historically the expansion of aquaculture has, however, been accompanied by the increased incidence of economically significant diseases, most notably of viral and bacterial origin. Since the late 1970s great progress has been made in our understanding of the generalized protostome innate immune system. Distinct pathways, pathogen receptor proteins and effector molecules have since been identified that are not ancestral or homologous to those of the deuterostomes, including vertebrates. Within the past decade progress has accelerated with the rapid characterisation of new classes of recognition proteins, immune effectors and regulatory pathways. This paper provides a broad overview of our current understanding of invertebrate immunology, taking the crustacean decapod immune system as its focus. Recent developments in the field are described briefly and their implications and potential considered. These advances offer fundamental new insights in our efforts to understand disease in cultured populations and also to develop knowledge of environmental effects on host/pathogen interactions within a fishery context. Of course, challenges do remain, including the lack of an immortal cell line and the limited publically-available genomic resources. These are considered in this review as priorities for future research effort. With the continued application of more insightful technologies, coupled with associated investment, it is expected that the speed at which some of these issues are resolved will accelerate.

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Structural and functional differences of Litopenaeus vannamei crustins

Cited by 107 publications

References 52 publications

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV

Adaptive immunity in invertebrates: A straw house without a mechanistic foundation

The scope of the crustacean immune system for disease control

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