Structural and functional diversity of biofilm bacterial communities along the Pearl River Estuary, South China

Mai, Yongzhan; Peng, Songyao; Lai, Zhigang

doi:10.1016/j.rsma.2019.100926

Cited by 11 publications

(8 citation statements)

References 70 publications

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“…In this study, the main bacterial phylum in the abundant and rare taxa was Proteobacteria in the sampling region (Figure 1). The distribution pattern of Proteobacteria described here is consistent with previous studies in the Amazon River Estuary Santos-Júnior et al, 2020), the Pearl River Estuary (Mai et al, 2020) and the Yellow River estuary (Wang et al, 2021). Proteobacteria not only exhibit a broad spectrum of metabolic diversity (e.g., photosynthetic metabolism, sulfate reduction, nitrogen metabolism) but also fulfil critical ecological roles, such as the decomposition of organic matter within the carbon cycle and the biodegradation of contaminants.…”

Section: Discussionsupporting

confidence: 89%

Rare subcommunity maintains the stability of ecosystem multifunctionality by deterministic assembly processes in subtropical estuaries

Yang,

Hou,

et al. 2024

Front. Microbiol.

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Microorganisms, especially rare microbial species, are crucial in estuarine ecosystems for driving biogeochemical processes and preserving biodiversity. However, the understanding of the links between ecosystem multifunctionality (EMF) and the diversity of rare bacterial taxa in estuary ecosystems remains limited. Employing high-throughput sequencing and a variety of statistical methods, we assessed the diversities and assembly process of abundant and rare bacterioplankton and their contributions to EMF in a subtropical estuary. Taxonomic analysis revealed Proteobacteria as the predominant phylum among both abundant and rare bacterial taxa. Notably, rare taxa demonstrated significantly higher taxonomic diversity and a larger species pool than abundant taxa. Additionally, our findings highlighted that deterministic assembly processes predominantly shape microbial communities, with heterogeneous selection exerting a stronger influence on rare taxa. Further analysis reveals that rare bacterial beta-diversity significantly impacts to EMF, whereas alpha diversity did not. The partial least squares path modeling (PLS-PM) analysis demonstrated that the beta diversity of abundant and rare taxa, as the main biotic factor, directly affected EMF, while temperature and total organic carbon (TOC) were additional key factors to determine the relationship between beta diversity and EMF. These findings advance our understanding of the distribution features and ecological knowledge of the abundant and rare taxa in EMF in subtropical estuaries, and provide a reference for exploring the multifunctionality of different biospheres in aquatic environments.

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Section: Discussionsupporting

confidence: 89%

Rare subcommunity maintains the stability of ecosystem multifunctionality by deterministic assembly processes in subtropical estuaries

Yang,

Hou,

et al. 2024

Front. Microbiol.

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“…Periphyton filtration has been developed to eliminate or decrease pollutants (e.g., organic contaminants, heavy metals, and antibiotics) from natural water environments and wastewater ( Matos et al, 2014 ; Liu et al, 2018a ). Periphytic biofilms are complex microorganism communities entwined together by extracellular polymeric substrates (EPS; Douterelo et al, 2018 ), mainly comprised of bacteria, fungi, microalgae, epiphytes, and detritus ( Liu et al, 2018a ; Mai et al, 2020 ). In periphytons, the autotrophic microbial assemblages play a major role in primary productivity and autochthonous carbon production ( Calapez et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…In periphytons, the autotrophic microbial assemblages play a major role in primary productivity and autochthonous carbon production ( Calapez et al, 2020 ). Moreover, heterotrophic communities can effectively biodegrade and remove organic pollutants, promoting the metabolism, mineralisation, and circulation of essential nutrients in aquatic ecosystems ( Bondar-Kunze et al, 2016 ; Mai et al, 2020 ). Recently, to better utilise the periphytic biofilms’ capacity of biological purification and degradation, various kinds of artificial substrates have been applied to immobilise periphytons, substituting natural substrates ( Witt et al, 2011 ; An et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…For example, carbon fibres (CFs; An et al, 2018 ; Liu et al, 2018a ), activated CF (ACF; Chen et al, 2013 ), and polyvinyl chloride (PVC) plastic ( Xia et al, 2010 ; Liu et al, 2014 ; von Ammon et al, 2018 ) are widely used as carriers for microorganisms due to the advantages of their physicochemical properties (i.e., strong stability, superior biocompatibility, and large specific surface area). Furthermore, the effects of artificial substrates on periphytons have been analysed in several studies and compared to those on natural substrates, focusing on biomass, microbial diversity, and community structure ( Li et al, 2020a ; Mai et al, 2020 ), purification efficiency and the removal mechanisms of periphytons for different contaminants ( Matsumoto et al, 2012 ; Stern et al, 2017 ; An et al, 2018 ), and metabolic functions ( Boisson and Perrodin, 2006 ). Most previous studies only evaluated the bacterial communities developed on artificial substrates or natural substrates ( Besemer, 2015 ; Miao et al, 2019 ; Oberbeckmann and Labrenz, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Periphytic Biofilm Formation on Natural and Artificial Substrates: Comparison of Microbial Compositions, Interactions, and Functions

et al. 2021

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Periphytic biofilms have been widely used in wastewater purification and water ecological restoration, and artificial substrates have been progressively used for periphyton immobilisation to substitute natural substrates. However, there is insufficient knowledge regarding the interaction network structure and microbial functions in biofilm communities on artificial substrates, which are essential attribute affecting their applications in biofilm immobilisation. This study compared the community structure, co-occurrence network, and metabolic functions of bacterial and microeukaryotic periphytic biofilms during a 35-day indoor cultivation on artificial substrates, such as artificial carbon fibre (ACF) and polyvinyl chloride (PVC), and natural substrates, such as pebble and wood. Results demonstrated that different types of artificial substrates could affect the community composition and functional diversity of bacterial and microeukaryotic biofilms. The bacterial and microeukaryotic community on ACF and PVC showed significantly higher Simpson index compared to those on wood. Bacterial networks on artificial substrates were more complex than those on natural substrates, while the keystone species on natural substrates were more abundant, indicating that the bacterial communities on artificial substrates had stronger stability and resistance to external interference. Furthermore, the functional metabolic profiles predicted showed the abilities of bacterial communities to metabolise nitrogen and carbon sources colonised on artificial substrates were stronger than those on natural substrates. These findings demonstrated that artificial substrates could be special niches for microbial colonisation, possibly altering microbial compositions, interactions, and functions. Therefore, this study provides a powerful theoretical basis for choosing suitable artificial substrates for microbial aggregation and immobilisation technology.

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“…Many factors can affect the colonization and community composition of biofilm, including substrate type, hydrodynamic force, nutrient concentration, and temperature [9,10,12]. The biofilm formation process is also influenced by various environmental factors, including conductivity, TOD (total oxygen demand) and salinity [13]. Studies have confirmed that the biofilm in different temperatures, lighting conditions or seasons showed various growth characteristics [14,15].…”

Section: Introductionmentioning

confidence: 99%

Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

Shen

Huang

Xie

et al. 2021

Water

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Increasing discharge of plastic debris into aquatic ecosystems and the worsening ecological risks have received growing attention. Once released, plastic debris could serve as a new substrate for microbes in waters. The complex relationship between plastics and biofilms has aroused great interest. To confirm the hypothesis that the presence of plastic in water affects the composition of biofilm in natural state, in situ biofilm culture experiments were conducted in a lake for 40 days. The diversity of biofilm attached on natural (cobble stones (CS) and wood) and plastic substrates (Polyethylene terephthalate (PET) and Polymethyl methacrylate (PMMA)) were compared, and the community structure and composition were also analyzed. Results from high-throughput sequencing of 16S rRNA showed that the diversity and species richness of biofilm bacterial communities on natural substrate (observed species of 1353~1945, Simpson index of 0.977~0.989 and Shannon–Wiener diversity index of 7.42~8.60) were much higher than those on plastic substrates (observed species of 900~1146, Simpson index of 0.914~0.975 and Shannon–Wiener diversity index of 5.47~6.99). The NMDS analyses were used to confirm the taxonomic significance between different samples, and Anosim (p = 0.001, R = 0.892) and Adonis (p = 0.001, R = 808, F = 11.19) demonstrated that this classification was statistically rigorous. Different dominant bacterial communities were found on plastic and natural substrates. Alphaproteobacterial, Betaproteobacteria and Synechococcophycideae dominated on the plastic substrate, while Gammaproteobacteria, Phycisphaerae and Planctomycetia played the main role on the natural substrates. The bacterial community structure of the two substrates also showed significant difference which is consistent with previous studies using other polymer types. Our results shed light on the fact that plastic debris can serve as a new habitat for biofilm colonization, unlike natural substrates, pathogens and plastic-degrading microorganisms selectively attached to plastic substrates, which affected the bacterial community structure and composition in aquatic environment. This study provided a new insight into understanding the potential impacts of plastics serving as a new habitat for microbial communities in freshwater environments. Future research should focus on the potential impacts of plastic-attached biofilms in various aquatic environments and the whole life cycle of plastics (i.e., from plastic fragments to microplastics) and also microbial flock characteristics using microbial plastics in the natural environment should also be addressed.

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Structural and functional diversity of biofilm bacterial communities along the Pearl River Estuary, South China

Cited by 11 publications

References 70 publications

Rare subcommunity maintains the stability of ecosystem multifunctionality by deterministic assembly processes in subtropical estuaries

Rare subcommunity maintains the stability of ecosystem multifunctionality by deterministic assembly processes in subtropical estuaries

Periphytic Biofilm Formation on Natural and Artificial Substrates: Comparison of Microbial Compositions, Interactions, and Functions

Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

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