Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins

Wu, Yung-Fu; Shien, Jui Huang; Yin, Hao; Chiow, Shiou Her; Lee, Long Huw

doi:10.1016/j.vetimm.2008.03.001

Cited by 27 publications

(15 citation statements)

References 25 publications

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“…IL-8 attracts neutrophils and naïve T cells to the site of infection [48]. Bursal infiltration of T cells during IBDV infection is coincident with the upregulation of chicken IL-8 [21], indicating that chicken IL-8 may help recruit T cells to the bursa.…”

Section: Discussionmentioning

confidence: 99%

Role of chicken melanoma differentiation-associated gene 5 in induction and activation of innate and adaptive immune responses to infectious bursal disease virus in cultured macrophages

Lee

Lin

2015

Arch Virol

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The objective of the present study was to determine if chicken melanoma-differentiation-associated gene 5 (MDA5) senses infectious bursal disease virus infection to induce innate immunity that bridges to adaptive immunity. During IBDV infection in HD11 cells, IBDV titers and RNA loads increased up to 3.4 × 10(7) plaque-forming units (PFU)/mL and 1114 ng/µL, respectively, at 24 hours postinfection (hpi). IBDV infection in HD11 cells induced significantly upregulated (p < 0.05) expression levels of chicken MDA5 (59-fold), interferon-β (IFN-β) (693-fold), dsRNA-dependent protein kinase (PKR) (4-fold), 2', 5'-oligoadenylate synthetase (OAS) (286-fold), myxovirus resistance gene (Mx) (22-fold), interleukin-1β (IL-1β) (5-fold), IL-6 (146-fold), IL-8 (4-fold), IL-10 (4-fold), inducible nitric oxide synthase (iNOS) (15-fold), and major histocompatibility complex class I (MHC class I) (4-fold). Nitric oxide production in the culture supernatants increased significantly (p < 0.05) up to 6.5 μM at 24 hpi. The expressed chMDA5 and IBDV-derived dsRNA were localized in the cytoplasm of HD11 cells during IBDV infection. ChMDA5-knockdown HD11 cells had significantly higher (p < 0.05) IBDV RNA loads at 24 hpi and significantly lower (p < 0.05) nitric oxide production and expression levels of chicken MDA5, IFN-β, PKR, OAS, Mx, IL-1β, IL-6, IL-8, IL-12(p40), IL-18, IL-10, iNOS, MHC class I and CD86 at 24 hpi. In addition, chMDA5 overexpression in HD11 cells resulted in significantly reduced (p < 0.05) IBDV titers and RNA loads and significantly increased (p < 0.05) nitric oxide production at 16 and 24 hpi. It also resulted in significantly higher (p < 0.05) expression levels of chicken MDA5, IFN-β, PKR, OAS, Mx, IL-1β, IL-6, IL-8, IL-12(p40), IL-10 and iNOS at 2 hpi. In conclusion, the results indicate that chMDA5 senses IBDV infection in chicken macrophages, and this is associated with IBDV-induced expression of IFN-β and initiation of an innate immune response that in turn activates the adaptive immune response and limits IBDV replication.

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Section: Discussionmentioning

confidence: 99%

Role of chicken melanoma differentiation-associated gene 5 in induction and activation of innate and adaptive immune responses to infectious bursal disease virus in cultured macrophages

Lee

Lin

2015

Arch Virol

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“…The upregulation of these genes indicates the ability of FPV to elicit proinflammatory responses leading to the recruitment and activation of adaptive immune system cells. IL-1␤ and IL-8 both are proinflammatory cytokines whose function has been studied in the chicken (61,62). The expression of both cytokines is enhanced following infection with certain viruses, such as reovirus and infectious bursal disease virus (27,28,61).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Host Responses against a Recombinant Fowlpox Virus-Vectored Vaccine Expressing the Hemagglutinin Antigen of an Avian Influenza Virus

Hghihghi

Read

Mohammadi

et al. 2010

Clin Vaccine Immunol

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There currently are commercial fowlpox virus (FPV)-vectored vaccines for use in chickens, including TROVAC-AIV H5, which expresses the hemagglutinin (HA) antigen of an avian influenza virus and can confer immunity against avian influenza in chickens. Despite the use of recombinant FPV (rFPV) for vaccine delivery, very little is known about the immune responses generated by these viruses in chickens. The present study was designed to investigate host responses to rFPV in vivo and in vitro. In cultured cells infected with TROVAC-AIV H5, there was an early increase in the expression of type I interferons (IFN), Toll-like receptors 3 and 7 (TLR3 and TLR7, respectively), TRIF, and MyD88, which was followed by a decrease in the expression of these genes at later time points. There also was an increase in the expression of interleukin-1␤ (IL-1␤), IL-8, and beta-defensin genes at early time points postinfection. In chickens immunized with TROVAC-AIV H5, there was higher expression of IFN-␥ and IL-10 at day 5 postvaccination in spleen of vaccinated birds than in that of control birds. We further investigated the ability of the vaccine to induce immune responses against the HA antigen and discovered that there was a cell-mediated response elicited in vaccinated chickens against this antigen. The findings of this study demonstrate that FPV-vectored vaccines can elicit a repertoire of responses marked by the early expression of TLRs, type I interferons, and proinflammatory cytokines, as well as cytokines associated with adaptive immune responses. This study provides a platform for designing future generations of rFPV-vectored vaccines.

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“…Early studies showed that chicken IL-8 protein was chemotactic for both peripheral blood mononuclear cells and heterophils (the avian equivalent of the neutrophils) [29]. Recently, chemotaxis assays showed that lymphocytes were the targets which were recruited toward recombinant IL-8 (rIL-8) proteins of five avian species (chicken, duck, goose, turkey and pigeon, ELR + ) [14]. Interestingly, to date, none of the teleost IL-8 sequences has this ELR motif except haddock (M. aeglefinus) and Atlantic cod (G. morhua) (two members of the gadoid family) [17,18].…”

Section: Discussionmentioning

confidence: 99%

“…[2]). Chicken IL-8 is the first identified bird chemokine, and recently, the duck, goose, turkey and pigeon IL-8 proteins have been reported [14]. Despite the lack of published amphibian chemokine sequence, a hypothetical protein (GenBank accession no.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

Zhou

Guo

Dai

2009

Developmental & Comparative Immunology

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Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins

Cited by 27 publications

References 25 publications

Role of chicken melanoma differentiation-associated gene 5 in induction and activation of innate and adaptive immune responses to infectious bursal disease virus in cultured macrophages

Role of chicken melanoma differentiation-associated gene 5 in induction and activation of innate and adaptive immune responses to infectious bursal disease virus in cultured macrophages

Characterization of Host Responses against a Recombinant Fowlpox Virus-Vectored Vaccine Expressing the Hemagglutinin Antigen of an Avian Influenza Virus

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

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