Structural and functional multiplicity of the kisspeptin/GPR54 system in goldfish (Carassius auratus)

Li, Shuisheng; Zhang, Yong; Liu, Yun; Huang, Xigui; Huang, Weiren; Lu, Danqi; Zhu, Ping; Shi, Yu; Chen, Cheng; Liu, Xiaochun; Lin, Haoran

doi:10.1677/joe-09-0016

Cited by 178 publications

(185 citation statements)

References 39 publications

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“…Taken together, these data point to the existence of different levels of activation for the Kiss/Kissr systems in teleosts. When two Kiss receptors coexist in a fish species, both receptors may be activated by the two Kiss ligands with different potencies, indicating differential ligand selectivity (Biran et al (2008), Lee et al (2009), Li et al (2009, Kanda et al (2013) and Ohga et al (2013) and this study) and influencing physiological actions (Felip et al 2009, Kitahashi et al 2009, Li et al 2009). Of note, our results show that the sea bass Kiss1-derived peptides exhibit high potency to activate the human KISSR1.…”

Section: Discussionmentioning

confidence: 70%

“…In the southern bluefin tuna (Thunnus maccoyii) and in yellowtail kingfish (Seriola lalandi), Kissr3 showed a higher response to Kiss2-10 than to Kiss1-10, with stronger transduction via the PKC than PKA pathway (Nocillado et al 2012). Conversely, in goldfish, according to Li et al (2009), Kiss1-10 enhances the PKC pathway through Kissr3 activation, while Kiss2-10 exhibits higher preference for Kissr2 and the ability to activate both PKC and PKA pathways. It is interesting to note that the concentration of ligands used for receptor activation in sea bass was higher than in medaka, zebrafish and yellowtail kingfish, while the concentration of ligands for receptor activation in yellowtail kingfish and southern bluefin tuna showed to be higher than those used in medaka, zebrafish and chub mackerel.…”

Section: Discussionmentioning

confidence: 99%

“…Expression levels of kiss and kissr in the brain and, to a lesser extent, in the gonad have been analyzed in several non-mammalian species, including amphibians (Chianese et al 2013) and fish (Mohamed et al 2007, Nocillado et al 2007, Biran et al 2008, Filby et al 2008, van Aerle et al 2008, Kitahashi et al 2009, Mechaly et al 2009, Migaud et al 2012, Alvarado et al 2013, Ohga et al 2013, suggesting a putative role for kisspeptins in controlling reproduction. Moreover, in vivo studies in some teleosts have found that the systemic administration of kisspeptin forms induces detectable biological responses at brain and pituitary levels, with potencies depending on the species (Filby et al 2008, Felip et al 2009, Kitahashi et al 2009, Li et al 2009, Shi et al 2010, Espigares et al 2015. Furthermore, in vitro functional analyses have delineated ligand-receptor interactions in four fish species containing a duplicated Kiss system, namely zebrafish Danio rerio (Biran et al 2008), goldfish Carassius auratus (Li et al 2009), medaka Oryzias latipes (Kanda et al 2013) and chub mackerel Scomber japonicus (Ohga et al 2013), and in the orange-spotted grouper Epinephelus coioides with a single Kiss/Kissr pair (Shi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in vivo studies in some teleosts have found that the systemic administration of kisspeptin forms induces detectable biological responses at brain and pituitary levels, with potencies depending on the species (Filby et al 2008, Felip et al 2009, Kitahashi et al 2009, Li et al 2009, Shi et al 2010, Espigares et al 2015. Furthermore, in vitro functional analyses have delineated ligand-receptor interactions in four fish species containing a duplicated Kiss system, namely zebrafish Danio rerio (Biran et al 2008), goldfish Carassius auratus (Li et al 2009), medaka Oryzias latipes (Kanda et al 2013) and chub mackerel Scomber japonicus (Ohga et al 2013), and in the orange-spotted grouper Epinephelus coioides with a single Kiss/Kissr pair (Shi et al 2010). As is the case in mammals (Pinilla et al (2012) for review), the activation of PKC-MAPKs is involved in the signaling pathways used by the fish Kiss receptors, but different levels of activation have been observed, depending on the ligand/receptor combination .…”

Section: Introductionmentioning

confidence: 99%

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Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

et al. 2015

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Two forms of kiss gene (kiss1 and kiss2) have been described in the teleost sea bass. This study assesses the cloning and characterization of two Kiss receptor genes, namely kissr2 and kissr3 (known as gpr54-1b and gpr54-2b, respectively), and their signal transduction pathways in response to Kiss1 and Kiss2 peptides. Phylogenetic and synteny analyses indicate that these paralogs originated by duplication of an ancestral gene before teleost specific duplication. The kissr2 and kissr3 mRNAs encode proteins of 368 and 378 amino acids, respectively, and share 53.1% similarity in amino acid sequences. In silico analysis of the putative promoter regions of the sea bass Kiss receptor genes revealed conserved flanking regulatory sequences among teleosts. Both kissr2 and kissr3 are predominantly expressed in brain and gonads of sea bass, medaka and zebrafish. In the testis, the expression levels of sea bass kisspeptins and Kiss receptors point to a significant variation during the reproductive cycle. In vitro functional analyses revealed that sea bass Kiss receptor signals are transduced both via the protein kinase C and protein kinase A pathway. Synthetic sea bass Kiss1-15 and Kiss2-12 peptides activated Kiss receptors with different potencies, indicating a differential ligand selectivity. Our data suggest that Kissr2 and Kissr3 have a preference for Kiss1 and Kiss2 peptides, respectively, thus providing the basis for future studies aimed at establishing their physiologic roles in sea bass. . To date, a single ligand (Kiss) and receptor (Gpr54 or Kissr) have been demonstrated to exist in placental mammals (human, opossum) and reptiles, while mammalian monotrems (platypus) possess two forms of Kiss and Kissr genes. Contrasting situations are found in other tetrapodes, such as amphibians, which have three kiss and kissr genes, whereas the kisspeptin system is absent in birds, although a recent investigation into the kisspeptin system in avian lineages provides molecular evidence of the presence of a kiss2-like gene that degenerated and lost its function (Pasquier et al. 2014b). All in all, advances in genome sequencing and comparative genomics in several groups occupying relevant phylogenetic positions have led to the identification of four kissr and three kiss genes in the coelacanth (Sarcopterygian), while in teleosts at least one kiss and kissr is known to be present in all the species investigated to date. Nevertheless, a second gene for kisspeptin and its receptor has been observed in the genomes of other fish species (Biran et al. 2008, Akazome et al. 2010, Oakley et al. 2010, Um et al. 2010, Zohar et al. 2010, Kim et al. 2012, Pasquier et al. 2012a,b, Tena-Sempere et al. 2012. So far, the occurrence of three kissr and two kiss paralogous genes has been reported in an early group of teleosts, the Elopomorphs (European eel, Anguilla anguilla) (Pasquier et al. 2012a,b). In the spotted gar, Lepisosteus oculatus (Actinopterygian, Ginglymode), four kissr and two kiss have been identified, while four kiss but n...

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

et al. 2015

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“…While the robust stimulatory effects of GnRH on LH production are well established in teleost as in other vertebrates, the role of the kisspeptins is poorly understood. There is evidence for stimulatory effects of kisspeptin on LH transcription and secretion (Felip et al, 2009;Kitahashi et al, 2009;Li et al, 2009;Zmora et al, 2012). However, kisspeptin is an inhibitor of LH expression in eel (Anguilla anguilla) (Pasquier et al, 2011).…”

Section: Changes In Hypothalamic Gene Expression Do Not Explain Reducmentioning

confidence: 99%

Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.

Cao

Chen

Wei

et al. 2014

General and Comparative Endocrinology

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Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish

Pei²,

Yang

2019

FEBS Open Bio

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Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals, fishes often possess more than one copy of the kiss and gpr54 genes. Our objectives were to identify all kiss/gpr54 genes in the genome of M. albus and to assess their involvement in sex reversal via their expression patterns (qPCR) in females, males, and intersex specimens. We identified only two genes: kiss2 and gpr54‐2. kiss2 expression was extremely high in the gonads of males, intermediate in females, and low in intersex; and reduced in all tissues of intersex. gpr54 expression was also extremely high in the gonads of males, high in intersex, but low in females. gpr54 expression in brain was high in all three sexes. In conclusion, (a) kiss1 has been functionally replaced in M. albus; (b) the functions of gpr54‐2 in brain are not sex‐specific; (c) kiss2 appears to undergo a ‘reset’ in the expression during the sex change; and (d) sex‐specific expression patterns in the gonads indicate that these two genes may play a role in sex reversal in fish.

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Structural and functional multiplicity of the kisspeptin/GPR54 system in goldfish (Carassius auratus)

Cited by 178 publications

References 39 publications

Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

Differential activation of kiss receptors by Kiss1 and Kiss2 peptides in the sea bass

Effects of growth hormone over-expression on reproduction in the common carp Cyprinus carpio L.

Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish

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