Structural and kinetic studies on β-lactamase K1 from <i>Klebsiella aerogenes</i>

Emanuel, E L; Gagnon, Jean; Waley, S. G.

doi:10.1042/bj2340343

Cited by 16 publications

(17 citation statements)

References 36 publications

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“…The high homology of nucleotide sequences between the&lactamase gene of K. pneumoniae LEN-l and the TEM ,&lactamase gene of Tn3 clearly shows that these two kinds of ,&-lactamases originate from a common ancestor and belong to class A. Our results based on the nucleotide sequence analysis are consistent with the recent finding by Emanuel et al [14] that the fl-lactamase of K. aerogenes (K. pneumoniae) related to class A fllactamases on the basis of the limited amino acid sequence analysis of the peptide. The relatively low homology in the 60 amino acid sequence from Nterminal and 20 amino acid from C-terminal (36 and 43% homology, respectively) suggests that these portions do not play essential roles in their enzymatic activities.…”

Section: Hybridizationsupporting

confidence: 92%

Close evolutionary relationship between the chromosomally encoded β lactamase gene of Klebsiellapneumoniae and the TEM β‐lactamase gene mediated by R plasmids

et al. 1986

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Sixty-three percent homology of nucleotide sequence and 67% homology of deduced amino acid sequence were found between the chromosomally encoded p-lactamase gene of Klebsiella pneumoniae and the TEM p-lactamase of transposon Tn3. Moreover, 22 out of 24 amino acid residues are identical around the predicted active site. It is therefore suggested that these two kinds of /?-lactamases share a common evolutionary origin. The 0.5 kb DNA fragment of the cloned gene hybridized specifically with the chromosomal DNA of all the K. pneumoniae strains tested which had been isolated in Japan, USA and Europe. P-Lactamase Nucleotide sequence Amino acid sequence(Klebsiella pneumoniae)

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Section: Hybridizationsupporting

confidence: 92%

Close evolutionary relationship between the chromosomally encoded β lactamase gene of Klebsiellapneumoniae and the TEM β‐lactamase gene mediated by R plasmids

et al. 1986

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“…Similar situations are certain to arise in the future with enzymes that have not been examined by using the z Amino acid sequences of active-site peptides of K1 enzymes from 1082E and SC10436 differed only at the residue preceding the active site serine: asparagine in strain 1082E and cysteine in strain SC10436. Substitutions were compatible with differential susceptibilities to thiol group reagents (82,135).…”

Section: Discussionmentioning

confidence: 99%

A functional classification scheme for beta-lactamases and its correlation with molecular structure

Bush¹,

Jacoby²,

Medeiros³

1995

Antimicrob Agents Chemother

2,225

1,680

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“…K. oxytoca is naturally resistant to aminoand carboxy-penicillins (21), a phenotype due to the constitutive expression of a chromosomal class A ␤-lactamase (1), first called K1 (7,20) or KOXY (26) and now OXY (12). Due to the hyperproduction of the chromosomal ␤-lactamase, up to 10 to 20% of K. oxytoca strains (22) can show high-level resistance to certain expanded-spectrum cephalosporins (ceftriaxone and cefotaxime) and aztreonam (8).…”

mentioning

confidence: 99%

Six Groups of the OXY β-Lactamase Evolved over Millions of Years in Klebsiella oxytoca

Fèvre

Jbel

Passet

et al. 2005

Antimicrob Agents Chemother

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The diversity and evolution of the class A OXY ␤-lactamase from Klebsiella oxytoca were investigated and compared to housekeeping gene diversity. The entire bla OXY coding region was sequenced in 18 clinical isolates representative of the four K. oxytoca ␤-lactamase gene groups bla OXY-1 to bla OXY-4 and of two new groups identified here, bla OXY-5 (with four isolates with pI 7.2 and one with pI 7.7) and bla OXY-6 (with four isolates with pI 7.75 and three with pI 8.1). Genes bla OXY-5 and bla OXY-6 showed 99.8% within-group nucleotide similarity but differed from each other by 4.2% and from bla OXY-1 , their closest relative, by 2.5% and 2.9%, respectively. Antimicrobial susceptibility to ␤-lactams was similar among OXY groups. Nucleotide sequence diversity of the 16S rRNA (1,454 bp), rpoB (940 bp), gyrA (383 bp), and gapDH (573 bp) genes was in agreement with the ␤-lactamase gene phylogeny. Strains with bla OXY-1 , bla OXY-2 , bla OXY-3 , bla OXY-4 , and bla OXY-6 genes formed five phylogenetic groups, named KoI, KoII, KoIII, KoIV, and KoVI, respectively. Isolates harboring bla OXY-5 appeared to represent an emerging lineage within KoI. We estimated that the bla OXY gene has been evolving within K. oxytoca for approximately 100 million years, using as calibration the 140-million-year estimation of the Escherichia coli-Salmonella enterica split. These results show that the bla OXY gene has diversified along K. oxytoca phylogenetic lines over long periods of time without concomitant evolution of the antimicrobial resistance phenotype.Klebsiella oxytoca is an important opportunistic pathogen causing serious infections in hospitalized patients, including neonates (16,19,28). K. oxytoca is naturally resistant to aminoand carboxy-penicillins (21), a phenotype due to the constitutive expression of a chromosomal class A ␤-lactamase (1), first called K1 (7,20) or KOXY (26) and now OXY (12). Due to the hyperproduction of the chromosomal ␤-lactamase, up to 10 to 20% of K. oxytoca strains (22) can show high-level resistance to certain expanded-spectrum cephalosporins (ceftriaxone and cefotaxime) and aztreonam (8). Up mutations in the promoter sequence of the genes are responsible for this phenotype (9-11). Overproducers of OXY enzymes are commonly resistant to all combinations of ␤-lactams with ␤-lactam inhibitors (21).Sequence diversity of the K. oxytoca chromosomal ␤-lactamase gene and the existence of discrete groups of OXY enzymes have been described. Fournier et al. (12) found a variant (bla OXY-2 ) of the chromosomal ␤-lactamase gene that differed from bla OXY-1 (1) by 12.7% in nucleotide sequence. It was suggested, based on colony hybridization, that the ␤-lactamase genes of K. oxytoca could be classified into two groups (OXY-1 and OXY-2), each representing approximately half of the clinical isolates (12). Recently, Granier et al. (14) identified two additional sequence variants, defined as bla OXY-3 and bla , each found in a single strain so far. Gene bla shares 85 and 84% similarity with bla OXY-1 and bla OXY...

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Structural and kinetic studies on β-lactamase K1 from Klebsiella aerogenes

Cited by 16 publications

References 36 publications

Close evolutionary relationship between the chromosomally encoded β lactamase gene of Klebsiellapneumoniae and the TEM β‐lactamase gene mediated by R plasmids

Close evolutionary relationship between the chromosomally encoded β lactamase gene of Klebsiellapneumoniae and the TEM β‐lactamase gene mediated by R plasmids

A functional classification scheme for beta-lactamases and its correlation with molecular structure

Six Groups of the OXY β-Lactamase Evolved over Millions of Years in Klebsiella oxytoca

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