Structural and mechanical characteristics of erythrocyte membranes in patients with type 2 diabetes mellitus

Стародубцева, М. Н.; Кузнецова, Т. Г.; Yegorenkov, N.I.; Sn, Cherenkevich

doi:10.1007/s10517-008-0036-3

Cited by 16 publications

(10 citation statements)

References 10 publications

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“…Structural and biochemical changes of RBC that cause alterations in the cytoskeleton proteins may lead to degradation of the cell. Modifications like glycation and oxidation of spectrin have been documented in diabetes mellitus and associated to erythrocyte membrane changes [26]–[27]. We recently could demonstrate glycosylation and proteolytic cleavage of spectrin in RBC of VL patients [11].…”

Section: Introductionmentioning

confidence: 79%

Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis

et al. 2012

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Visceral leishmaniasis (VL) caused by the intracellular parasite Leishmania donovani accounts for an estimated 12 million cases of human infection. It is almost always associated with anemia, which severely complicates the disease course. However, the pathological processes leading to anemia in VL have thus far not been adequately characterized to date. In studying the glycosylation patterns of peripheral blood cells we found that the red blood cells (RBC) of VL patients (RBCVL) express eight 9-O-acetylated sialoglycoproteins (9-O-AcSGPs) that are not detected in the RBC of healthy individuals (RBCN). At the same time, the patients had high titers of anti-9-O-AcSGP IgG antibodies in their sera. These two conditions appear to be linked and related to the anemic state of the patients, as exposure of RBCVL but not RBCN to anti-9-O-AcSGPs antibodies purified from patient sera triggered a series of responses. These included calcium influx via the P/Q-type but not L-type channels, activation of calpain I, proteolysis of spectrin, enhanced oxidative stress, lipid peroxidation, externalization of phosphatidyl serine with enhanced erythrophagocytosis, enhanced membrane fragility and, finally, hemolysis. Taken together, this study suggests that the enhanced hemolysis is linked to an impairment of membrane integrity in RBCVL which is mediated by ligand-specific interaction of surface 9-O-AcSGPs. This affords a potential explanation for the structural and functional features of RBCVL which are involved in the hemolysis related to the anemia which develops in VL patients.

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Section: Introductionmentioning

confidence: 79%

Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis

et al. 2012

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“…Baines in 2009 suggested that the elasticity of RBCs depends on the dynamic rearrangement of spectrin dimers/tetramers under the shearing forces experienced in circulation (Baines, 2009); and that stable intact helical linker regions are needed to maintain the soft elasticity of spectrin (Mirijanian and Voth, 2008). A decreased deformability is also seen in inflammatory conditions such as diabetes (Shin et al, 2007); a spatial reorganization of the cytoskeleton was also noted in this condition (Starodubtseva et al, 2008). A reduced plasticity in cerebral ischemia (Kowal, 1996) and increase stiffness was also noted in conditions like coronary disease and hypertension (Lekka et al, 2005).…”

Section: Discussionmentioning

confidence: 92%

High ferritin levels have major effects on the morphology of erythrocytes in Alzheimer's disease

Bester

Buys

Lipiński

et al. 2013

Front. Aging Neurosci.

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Introduction: Unliganded iron both contributes to the pathology of Alzheimer's disease (AD) and also changes the morphology of erythrocytes (RBCs). We tested the hypothesis that these two facts might be linked, i.e., that the RBCs of AD individuals have a variant morphology, that might have diagnostic or prognostic value. Methods:We included a literature survey of AD and its relationships to the vascular system, followed by a laboratory study. Four different microscopy techniques were used and results statistically compared to analyze trends between high and normal serum ferritin (SF) AD individuals.Results: Light and scanning electron microscopies showed little difference between the morphologies of RBCs taken from healthy individuals and from normal SF AD individuals. By contrast, there were substantial changes in the morphology of RBCs taken from high SF AD individuals. These differences were also observed using confocal microscopy and as a significantly greater membrane stiffness (measured using force-distance curves). Conclusion:We argue that high ferritin levels may contribute to an accelerated pathology in AD. Our findings reinforce the importance of (unliganded) iron in AD, and suggest the possibility both of an early diagnosis and some means of treating or slowing down the progress of this disease.

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“…66,67 RBCs play a fundamental role in the inflammatory process including having a changed deformability, rheology or sedimentation rate. 53,65,[67][68][69][70][71][72][73][74][75][76][77][78][79][80] Central to cardiovascular health is the optimal functioning of RBCs, and central to a variety of vascular and degenerative diseases is the presence of poorly liganded iron [81][82][83] some of which comes from erythrocytes, and which leads to a variety of morphological and phenotypic manifestations. Therefore in this review, we will be taking an integrative approach that focuses on the use of biophysical properties of RBCs and fibrin(ogen) as diagnostic indicators of health.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic morphology: biophysical indicators for iron-driven inflammatory diseases

2014

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Most non-communicable diseases involve inflammatory changes in one or more vascular systems, and there is considerable evidence that unliganded iron plays major roles in this. Most studies concentrate on biochemical changes, but there are important biophysical correlates. Here we summarize recent microscopy-based observations to the effect that iron can have major effects on erythrocyte morphology, on erythrocyte deformability and on both fibrinogen polymerization and the consequent structure of the fibrin clots formed, each of which contributes significantly and negatively to such diseases. We highlight in particular type 2 diabetes mellitus, ischemic thrombotic stroke, systemic lupus erythematosus, hereditary hemochromatosis and Alzheimer's disease, while recognizing that many other diseases have co-morbidities (and similar causes). Inflammatory biomarkers such as ferritin and fibrinogen are themselves inflammatory, creating a positive feedback that exacerbates disease progression. The biophysical correlates we describe may provide novel, inexpensive and useful biomarkers of the therapeutic benefits of successful treatments.

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Structural and mechanical characteristics of erythrocyte membranes in patients with type 2 diabetes mellitus

Cited by 16 publications

References 10 publications

Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis

Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis

High ferritin levels have major effects on the morphology of erythrocytes in Alzheimer's disease

Diagnostic morphology: biophysical indicators for iron-driven inflammatory diseases

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