Structural and Serological Studies of the O6-Related Antigen of Aeromonas veronii bv. sobria Strain K557 Isolated from Cyprinus carpio on a Polish Fish Farm, which Contains l-perosamine (4-amino-4,6-dideoxy-l-mannose), a Unique Sugar Characteristic for Aeromonas Serogroup O6

Abstract

Amongst Aeromonas spp. strains that are pathogenic to fish in Polish aquacultures, serogroup O6 was one of the five most commonly identified immunotypes especially among carp isolates. Here, we report immunochemical studies of the lipopolysaccharide (LPS) including the O-specific polysaccharide (O-antigen) of A. veronii bv. sobria strain K557, serogroup O6, isolated from a common carp during an outbreak of motile aeromonad septicemia (MAS) on a Polish fish farm. The O-polysaccharide was obtained by mild acid degradation of the LPS and studied by chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-antigen was composed of two O-polysaccharides, both containing a unique sugar 4-amino-4,6-dideoxy-L-mannose (N-acetyl-L-perosamine, L-Rhap4NAc). The following structures of the O-polysaccharides (O-PS 1 and O-PS 2) were established:

A First Report of Aeromonas veronii Infection of the Sea Bass, Lateolabrax maculatus in China

“…A. veronii is widely distributed and can be isolated from diseased aquatic animals and aquatic environments (17)(18)(19) in various countries such as Poland (20), Mexico (21), and Japan (22). The pathogen can cause human biliary sepsis and diarrhea in clinical practice (23,24).…”

Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv. sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms

“…sobria K133 lipopolysaccharide ( Figure 2 a) showed the most intensive signals in the m / z range 1600–2000, which were attributable to the lipid A and core oligosaccharide species (Y- and B-type fragment ions) arising from in-source fragmentation at the ketosidic bond between the core oligosaccharide and the lipid A [ 33 ]. The ions at m / z 1768.19, 1796.23, and 1824.26 corresponded to hexaacylated lipid A species (Y-fragment ions) [ 25 ] composed of a bisphosphorylated glucosaminyl disaccharide and substituted by different chain-length fatty acids. The ion at m / z 1796.23 represented the lipid A species substituted by three 3-hydroxytetradecanoic acids C14:0(3-OH), one 3-hydroxy iso pentadecanoic acid C i 15:0(3-OH), and two tetradecanoic acids C14:0.…”

“…Serotyping of pathogenic bacteria leads to recognition of the etiological agent associated with specific disease syndrome [ 23 ]. Since there are differences in the distribution of the dominant O-serogroups responsible for the onset of various diseases depending on geographical regions [ 24 ], as evidenced by epidemiological studies carried out in various areas, including Polish aquacultures indicating to the emergence of new O-antigen variants, there are reasons to extend further or modify the classical serogrouping scheme [ 25 , 26 , 27 ].…”

Marine Glycoconjugates: Trends and Perspectives

“…Sobria K557 and the A. hydrophila JCM3968 O6 antiserum, and vice versa, is caused by the occurrence of common disaccharides, whereas an additional →4)-α-D-GalpNAc-associated epitope defines the specificity of the O6 reference antiserum. This investigation provides additional knowledge of the immunospecificity of Aeromonas bacteria and seems to be significant for epidemiological studies and for finding the routes of transmission of pathogenicity [19].…”

“…One article concerns the isolation of 10 new triterpene glycosides from a fungus associated with a sea cucumber [18]. The article by Dworaczek et al characterizes the O-specific polysaccharide (O-antigen) of a bacterial pathogen of common carp by chemical and immunochemical methods [19]. In total, the Special Issue comprises 13 articles, including two reviews.…”