Structural and ultrastructural characterization of male reproductive tracts and spermatozoa in fig wasps of the genus Pegoscapus (Hymenoptera, Chalcidoidea)

Fiorillo, Bruno Silva; Lino‐Neto, José; Báo, Sônia Nair

doi:10.1016/j.micron.2008.03.005

Cited by 20 publications

(37 citation statements)

References 36 publications

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“…They are much smaller than those of P. nasuta, which, at 665 m in length, are among the largest ever reported for Hymenoptera (Oliveira et al, 2010). However, the average nuclear length in both Bethylidae species is approximately 16 m. Similar ranges have been described for other groups of Hymenoptera, showing that closely related species may diverge widely in the length of the flagellum while maintaining similarity in the size of the nucleus (Lino-Neto et al, 2000;Lino-Neto and Dolder, 2001a;Fiorillo et al, 2008).…”

Section: Discussionsupporting

confidence: 71%

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Oliveira

Fraga

Brito

et al. 2013

Micron

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In this study, the morphology of spermatozoa of Bethylidae Dissomphalus connubialis (Pristocerinae) was analyzed using light and transmission electron microscopy. Spermatozoa of this species are thin, measure approximately 130 μm in length and comprise a head region and flagellum region. The head is formed by the acrosome and nucleus. The acrosome consists of the acrosome vesicle and the perforatorium, the posterior portion of which is inserted into a cavity at the anterior extremity of the nucleus. The nucleus is compact, electron-dense and measures 15 μm in length. The flagellum possesses two mitochondrial derivatives, two accessory bodies and one axoneme with a 9+9+2 microtubular pattern. The nucleus is connected to the flagellum by the centriole adjunct. Mitochondrial derivatives are compact, apparently without paracrystalline material and with rare mitochondrial cristae. They are asymmetric in length, such that the larger mitochondrial derivative begins parallel to the posterior region of the nucleus and the smaller mitochondrial derivative begins just below the centriole adjunct. The basic structure of spermatozoa of D. connubialis is similar to that of other Aculeata studied. However, this species shows characteristics not seen in other Hymenoptera, such as the wide electron-lucid region that separates the acrosomal vesicle from the perforatorium and the depth of the cavity in the anterior extremity of the nucleus, into which the base of the perforatorium is inserted. There are also characteristics that distinguish this species from Bethylidae Prorops nasuta, including the fact that one of the mitochondrial derivatives lies to parallel to the nucleus over a long distance, the small quantity of cristae, the absence of paracrystalline material in these organelles, and the fact that the accessory microtubules are the first to terminate in the final portion of the flagellum.

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Section: Discussionsupporting

confidence: 71%

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Oliveira

Fraga

Brito

et al. 2013

Micron

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“…Many similar morphological characteristics associated with the male reproductive system were found in comparative studies on the Hymenoptera (Araú jo et al., 2005a,b, 2009Brito et al, 2005;Bushrow et al, 2006;Dallacqua and Cruz-Landim, 2003;Damiens and Boivin, 2005;Ferreira et al, 2004;Fiorillo et al, 2008Fiorillo et al, , 2009Moreira et al, 2008Moreira et al, , 2010. On the other hand, these studies also identified several features that differed between the various groups.…”

Section: Discussionsupporting

confidence: 63%

“…In Formicidae, the number of follicles ranges from 1 to 25 (Wheeler and Krutzsch, 1992). A single follicle was found in each testis in some species of parasitic wasps (Fiorillo et al, 2008;Moreira et al, 2010), whereas in D. diversipes, there is 22 follicles. There are no reports in the literature for any of the other species of ''symphyta.…”

Section: Discussionmentioning

confidence: 99%

“…(Siricoidea), the centriolar adjunct begins below the nuclear base and ends, as in D. diversipes, surrounding the anterior tip of the two mitochondrial derivatives. In contrast to D. diversipes and A. pagana, in many Aculeata, the mitochondrial derivative originates above the nuclear base (Araú jo et al, 2005b;Fiorillo et al, 2008).…”

Section: Microscopy Research and Techniquementioning

confidence: 99%

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Morphological aspects of testes and sperm ultrastructure in the “symphyta” Digelasinus diversipes kirby 1882 (hymenoptera: Argidae: Dielocerinae)

Moreira

Araújo

Lino‐Neto

2011

Microscopy Res & Technique

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In Digelasinus diversipes, spermatozoa are maintained in bundles, with 74 spermatozoa on average, in the seminal vesicle. These spermatozoa are very short (20 μm) and consist of a head and flagellum. The head includes an acrosome (perforatorium covered by the acrosomal vesicle) and a nucleus. A regular electron-lucent region separates the acrosomal vesicle from the perforatorium, which is inserted parallel to the anterior ending of the nucleus. The small flagellum is composed of two symmetrical mitochondrial derivatives, a centriolar adjunct, an axoneme (9 + 9 + 2), and two accessory bodies. The centriolar adjunct begins above the posterior end of the nucleus and ends covering the anterior tip of two mitochondrial derivatives. In the terminal region of the axoneme, the central microtubules terminate first. The presence of a subacrosomal space, a short mitochondrial derivative diameter, and a short spermatodesm is the ultrastructure characteristics of spermatozoa shared by all "symphyta" species. Differences in the insertion of the perforatorium into the nucleus and the position of the centriolar adjunct distinguish Dielocerinae and the Arginae studied previously. The number of spermatozoa per cyst is variable. Furthermore, additional characteristics that had not been described for "symphyta" were also found, such as the number of follicles per testis.

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“…The occurrence of three follicles was reported in more basal bees such as Colletinae, Andreninae and Hactinae (Ferreira et al 2004, Araújo et al 2005, and wasps Crabronidae (Moreira et al 2008), considered a sister group to bees. On the other hand, this number varies from one to 11 in Formicidae (Wheeler & Krutzch 1992) and in wasps Chalcidoidea, where only one (Fiorillo et al 2008). The bees studied by Ferreira et al (2004) showed conspicuous variation in their reproductive system, even within the subfamily.…”

Section: Discussionmentioning

confidence: 99%

Morphology of male reproductive system of two solitary bee species (Hymenoptera: Apidae)

Araújo¹,

Freitas²,

Moreira³

et al. 2010

Neotrop. entomol.

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-Bees can form all levels of social organization, from solitary to advanced eusocial societies. Although 80% of the species exist as solitary species, most researches emphasize social species. This study focuses on the description of the male reproductive system of the solitary bees Thygater analis (Lepeletier) and Melitoma segmentaria (Fabricius) and searches for traits that support behavioral and phylogenetic studies. The reproductive system of males were dissected, fi xed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer pH 7.2 and post-fi xed with 1% osmium tetroxide. The tissue was dissected and included for light microscopy. The species presented similar anatomical traits, including paired testicles, seminal vesicles, deferent ducts, accessory glands and an ejaculatory duct. Each testicle was composed of four follicles. In both species, a testicle and a seminal vesicle were surrounded by a joint capsule, forming a seminal vesicle-testicle complex. The two species presented accessory glands with very distinct morphological traits. The anatomic pattern and the histological traits of the reproductive system of the species studied were similar to those described for other bees. In addition, these traits can be used to differentiate species and allow inferences concerning their reproductive biology.

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Structural and ultrastructural characterization of male reproductive tracts and spermatozoa in fig wasps of the genus Pegoscapus (Hymenoptera, Chalcidoidea)

Cited by 20 publications

References 36 publications

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Morphology of spermatozoa of Dissomphalus connubialis (Ashmead, 1893) (Hymenoptera: Bethylidae)

Morphological aspects of testes and sperm ultrastructure in the “symphyta” Digelasinus diversipes kirby 1882 (hymenoptera: Argidae: Dielocerinae)

Morphology of male reproductive system of two solitary bee species (Hymenoptera: Apidae)

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